We narrowed to 4,433 results for: chm

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pMGF169

Plasmid

#96996

Purpose

ish1 mCherry insertion cassette with G418 resistance gene

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

ish1 (ish1 Fission Yeast)

Tags

GST and mCherry

Expression

Bacterial

Mutation

ish1 C-terminal domain fused with mCherry + G418 …

Promoter

N/A

Available Since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pSG5-chCAR

Plasmid

#87231

Purpose

Mammalian expression of chicken CAR

Depositor

Fritz Rathjen

Article

Patzke et al J Neurosci. 2010 Feb 24;30(8):2897-910.

Insert

CXADR (CXADR Chicken)

Expression

Mammalian

Promoter

SV40

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156) GSGS

Plasmid

#61940

Purpose

Co-expression of hUCH37 (ISF1) and hNFRKB (1-156) GSGS in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Inserts

hUCH37 (ISF1) (UCHL5 Human)
hNFRKB (1-156) GSGS (NFRKB Human)

Tags

6x HIS

Expression

Bacterial

Mutation

Construct ends at residue 156, residues 97-100 (N…

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pETDUET1-hUCH37 (ISF1) - hNFRKB (1-117)

Plasmid

#61941

Purpose

Co-expression of hUCH37 (ISF1) and hNFRKB (1-117) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Inserts

hUCH37 (ISF1) (UCHL5 Human)
hNFRKB (1-117) (NFRKB Human)

Tags

6x HIS

Expression

Bacterial

Mutation

Construct ends at residue 117, stop codon introdu…

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
CROPseq-Guide-Puro-mCherry2

Plasmid

#219679

Purpose

CROPseq vector based on #86708 with an additional mCherry2 fluorescent gene

Depositor

Christoph Bock

Article

Boccuni et al Sci Signal. 2022 Dec 13;15(764):eabq5389

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7

Plasmid

#214812

Purpose

Mammalian expression of SpCas9 PE7 prime editor

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLX313-Renilla luciferase

Plasmid

#118016

Purpose

Constitutive expression of Renilla luciferase

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

Renilla luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP SNAP23

Plasmid

#101914

Purpose

Expresses SNAP23 in mammalian cells

Depositor

Thierry Galli

Article

Kuster et al J Biol Chem. 2015 Nov 20;290(47):28056-6

Insert

SNAP 23 (SNAP23 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-PE7 for IVT

Plasmid

#214813

Purpose

Template for in vitro transcription of PE7

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Promoter

T7 (inactivated)

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLX313-Firefly luciferase

Plasmid

#118017

Purpose

Constitutive expression of Firefly luciferase

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

Firefly luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG

Plasmid

#155305

Purpose

Lentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.

Depositor

Stanley Qi

Article

Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

Insert

mCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d

Use

Lentiviral

Tags

2xNLS-3xFLAG

Expression

Mammalian

Promoter

EF1a

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pIG-602_NY-ESO-1_TCR_RFP

Plasmid

#207483

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

RFP, NY-ESO-1_TCR

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pJ4M/TDP-43

Plasmid

#104480

Purpose

bacterial expression of TDP-43

Depositor

Nicolas Fawzi

Article

Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

TDP-43 (TARDBP Human)

Tags

MBP-6xHis

Expression

Bacterial

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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T7_CC_PE7_IVT_Template

Plasmid

#223022

Purpose

Template for in vitro transcription of PE7. For HSPC-related experiments.

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Promoter

T7

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO5d.SFFV.dCas9-KRAB.P2A.BSD

Plasmid

#90332

Purpose

CRISPR interference

Depositor

Dirk Heckl

Article

Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10

Inserts

dCas9
na
SFFV
P2A-BSD

Use

Lentiviral

Tags

HA

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP

Plasmid

#176027

Purpose

construct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometry

Depositor

Irmela Jeremias

Article

Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

Inserts

rtTA3
Luc2
mtagBFP

Use

Lentiviral

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 B.1.351v2

Plasmid

#169463

Purpose

Encodes SARS-CoV-2 B.1.351v2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.351v2 Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27A

Plasmid

#89237

Purpose

Expression of GFP-tagged human Rab27A in mammalian cells

Depositor

William Gahl

Article

Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

Insert

human Rab27A (RAB7A Human)

Tags

eGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-BAF

Plasmid

#101772

Purpose

Expresses EGFP tagged BAF in human cells

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Bcl2

Plasmid

#195551

Purpose

Expresses Bcl2 in mammalian cells

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Bcl2 (Bcl2 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Promoter

CMV enhancer and promoter

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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