19,634 results

1. pLenti PGK V5-LUC Puro (w543-1)

   Plasmid

   #19360

   Purpose

   3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Puro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   Firefly Luciferase
   

   Use

   Lentiviral and Luciferase

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOpen-ETSSB

   Plasmid

   #165549

   Purpose

   ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure

   Depositor

   Jennifer Molloy, Drew Endy, FreeGenes Project

   Insert

   Extreme Thermostable Single-Stranded DNA Binding Protein
   

   Use

   Synthetic Biology

   Available Since

   Aug. 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pMCSG17-NleL

   Plasmid

   #66716

   Purpose

   Expresses NleL (170-782) in E. coli

   Depositor

   David Komander

   Article

   Hospenthal et al Nat Struct Mol Biol. 2013 May;20(5):555-

   Insert

   NleL (NEWENTRY )
   

   Tags

   GST-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 24, 2015

   Availability

   Academic Institutions and Nonprofits only

4. mEmerald-Rab7a-7

   Plasmid

   #54244

   Purpose

   Localization: Ras GTPase, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Rab7a (RAB7A Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pcDNA3β-FLAG-CBP-HA

   Plasmid

   #32908

   Depositor

   Tso-Pang Yao

   Article

   Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

   Insert

   CBP (Crebbp Mouse)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. mito-V5-APEX2

   Plasmid

   #72480

   Purpose

   Expresses APEX2 in mitochondria

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   APEX2
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   April 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-puro/Flag PD-L1 WT

   Plasmid

   #121446

   Purpose

   expression of huamn PD-L1 wildtype in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   CD274 (CD274 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   wildtype

   Promoter

   CMV

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX302

   Plasmid

   #25896

   Purpose

   3rd generation lentiviral Gateway destination vector. Puromycin selection, V5 tag.

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Tags

   V5

   Expression

   Mammalian

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDESTmycDDX17

   Plasmid

   #19876

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (DDX17 Human)
   

   Tags

   MYC

   Expression

   Mammalian

   Available Since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAXY0002

    Plasmid

    #179835

    Purpose

    binary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoter

    Depositor

    Xiaohan Yang

    Article

    Yuan et al Hortic Res. 2021 Nov 1;8(1):234. doi: 10

    Type

    Empty backbone

    Use

    Binary vector for plant transformation

    Expression

    Plant

    Available Since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-Cas9-P2A-Puro

    Plasmid

    #110837

    Purpose

    Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9
    

    Use

    Lentiviral

    Mutation

    WT

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-BRAF-V600E

    Plasmid

    #116204

    Purpose

    Lentiviral expression of BRAF V600E

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    BRAF (BRAF Human)
    

    Use

    Lentiviral

    Mutation

    V600E

    Available Since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-IGF1R-V5/HIS

    Plasmid

    #201979

    Purpose

    expression of human IGF1R receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    IGF1R receptor tyrosine kinase (IGF1R Human)
    

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBABE puro human IRS-1

    Plasmid

    #11025

    Depositor

    Ronald Kahn

    Article

    Bruning et al Mol Cell Biol. 1997 Mar . 17(3):1513-21.

    Insert

    IRS-1 (IRS1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOPINS-AREL1

    Plasmid

    #66710

    Purpose

    Expresses AREL1 HECT domain in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Insert

    KIAA0317 (AREL1 )
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

16. plenti-CAG -IRES-GFP

    Plasmid

    #69047

    Purpose

    Lentiviral backbone for GFP coexpression

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mammalian

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. PGK-DTAG-NTERM-GFP

    Plasmid

    #185775

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, dTAG

    Mutation

    none

    Promoter

    PGK

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSIRV-NF-kB-eGFP

    Plasmid

    #118093

    Purpose

    Fluorescent reporter for NF-kB activity

    Depositor

    Peter Steinberger

    Article

    Jutz et al Oncotarget. 2017 May 4;8(39):64892-64906

    Insert

    eGFP
    

    Use

    Retroviral

    Promoter

    minimal promoter with NF-kB responsive element

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-gp32-H6

    Plasmid

    #163912

    Purpose

    Expresses T4 gp32 for bacterial expression and affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    gp32
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-ERBB2

    Plasmid

    #116734

    Purpose

    Lentiviral expression of ERBB2

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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