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p18sheLL

Plasmid

#37321

Depositor

John Schiller

Publication

Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

Insert

HPV18L1+L2

Expression

Mammalian

Promoter

CMV

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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FR_HNF4A2

Plasmid

#31100

Depositor

Gerhart Ryffel

Publication

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

HNF4A (HNF4A Human)

Tags

Myc

Expression

Mammalian

Mutation

splice variant 2

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pNH-TrxT

Plasmid

#26106

Purpose

SGC Empty backbone for bacterial expression

Depositor

Opher Gileadi

Publication

Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

Type

Empty backbone

Tags

6xHis, TEV cleavage site, and TrxA

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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CaV2.1 (pcDNA6)

Plasmid

#26578

Depositor

Diane Lipscombe

Publication

Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

Insert

Cacna1a (Cacna1a Mouse)

Tags

His6 and V5

Expression

Mammalian

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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Flag-HA-USP13

Plasmid

#22568

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP13 (USP13 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Flag-HA-USP20

Plasmid

#22573

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP20 (USP20 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAc5.1B-EGFP

Plasmid

#21181

Depositor

Elisa Izaurralde

Publication

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

enhanced green flourescent protein

Use

Drosophila expression

Available since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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GST-Pin1

Plasmid

#19027

Depositor

Michael Yaffe

Publication

Yaffe et al Science. 1997 Dec 12. 278(5345):1957-60.

Insert

Pin1 (PIN1 Human)

Tags

GST

Expression

Bacterial

Available since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pWZL hygro H-Ras V12

Plasmid

#18749

Depositor

Scott Lowe

Publication

Serrano et al Cell. 1997 Mar 7. 88(5):593-602.

Insert

H-Ras V12 (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

V12

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pSOUP

Plasmid

#165419

Purpose

The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Publication

Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

Insert

Rep A

Use

Helper plasmid required for replication for all p…

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-hSyn-FLEX-TeLC-P2A-dTomato

Plasmid

#159102

Purpose

Cre-dependent tetanus toxin light chain construct with nuclear-localized dTomato fluorophore for conditional silencing of neurons.

Depositor

Sandeep Datta

Publication

Pashkovski et al Nature. 2020 Jul;583(7815):253-258. doi:

Insert

TeLC-P2A-NLS-dTomato

Use

AAV

Promoter

hSynapsin

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pBA904

Plasmid

#122238

Purpose

Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.

Depositor

Jonathan Weissman

Publication

Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

Insert

sgRNA with cs1 in stem loop 2 of the constant region

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pcDNA3.1-SARS-Spike

Plasmid

#145031

Purpose

Express SARS-CoV spike protein with C9 tag at C-terminal in mammalian cells

Depositor

Fang Li

Publication

Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

Insert

SARS-CoV Spike (S Synthetic)

Tags

C9 and CD5 signal peptide

Expression

Mammalian

Promoter

CMV

Available since

April 16, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

13 Articles

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pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

Plasmid

#140002

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Publication

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

Promoter

CMV and T7

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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ER-mNeonGreen

Plasmid

#137804

Purpose

Visualization of the Endoplasmic Reticulum

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

KDEL

Expression

Mammalian

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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PLKO.1-Scrambled

Plasmid

#136035

Purpose

Scrambled shRNA (negative control) inserted into the PLKO.1 plasmid (CCTAAGGTTAAGTCGCCCTCG)

Depositor

Anthony Leung

Publication

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

None (Scrambled)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pLJM1-Tmem192-mRFP-3xHA

Plasmid

#134631

Purpose

bait for affinity-isolation of lysosomes

Depositor

Roberto Zoncu

Publication

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

TMEM192 (TMEM192 Human)

Use

Lentiviral

Tags

3xHA, TEV, and mRFP

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pAG153 FKBP-CFAST11

Plasmid

#130813

Purpose

Expresses FKBP-CFAST11 in mammalian cells

Depositor

Arnaud Gautier

Publication

Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

Insert

FKBP-CFAST11

Tags

Myc-tag

Expression

Mammalian

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

Plasmid

#128604

Purpose

Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Publication

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Inserts

24xMoonTag
kinesin family member 18b (KIF18B Human)

Expression

Mammalian

Promoter

Pcmv

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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4xmts-mNeonGreen

Plasmid

#98876

Purpose

In vivo visualization of mitochondria (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

4xmts

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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