4,178 results

1. pZac2.1 gfaABC1D-lck-GCaMP6f

   Plasmid

   #52924

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Baljit Khakh

   Has Service

   AAV5

   Insert

   lck-GCaMP6f
   

   Use

   AAV

   Tags

   6xHis

   Available Since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-sfCherry(1-10)

   Plasmid

   #70222

   Purpose

   Expresses sfCherry(1-10) in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   sfCherry(1-10)
   

   Expression

   Mammalian

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. phage ubc nls ha pcp gfp

   Plasmid

   #64539

   Purpose

   Used to label reporter mRNAs. Lentiviral expression of the PP7 coat protein fused to EGFP.

   Depositor

   Jeffrey Chao

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   PCP
   

   Use

   Lentiviral

   Tags

   EGFP, FactorXa site, HA, and NLS

   Expression

   Mammalian

   Promoter

   human ubiquitin C promoter

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-Flpe:GFP

   Plasmid

   #13788

   Purpose

   Mammalian expression of enhanced form of Flp recombinase fused to GFP

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Flpe-GFP fusion protein
   

   Use

   Flp/frt

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFJ151 - ttTi5605_MCS

   Plasmid

   #19330

   Depositor

   Erik Jorgensen

   Article

   Christian et al

   Insert

   ttTi5605 targeting region
   

   Expression

   Worm

   Available Since

   Oct. 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. PKC alpha CAT

   Plasmid

   #21234

   Depositor

   Bernard Weinstein

   Article

   Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

   Insert

   PKC alpha (PRKCA Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta 1-325

   Available Since

   Aug. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-BCL6-IRES-GFP

   Plasmid

   #31391

   Depositor

   Hilda Ye

   Article

   Yu et al Blood. 2005 Feb 15. 105(4):1777-84.

   Insert

   B-cell lymphoma 6 (BCL6 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSaCas9_GFP

   Plasmid

   #64709

   Purpose

   Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells

   Depositor

   Kiran Musunuru

   Insert

   SaCas9-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP and 3xFLAG-SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRK669

   Plasmid

   #71313

   Purpose

   Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.

   Depositor

   Rodolphe Barrangou, Todd Klaenhammer

   Article

   Russell et al Appl Environ Microbiol. 2001 Sep;67(9):4

   Type

   Empty backbone

   Use

   E. coli-gram positive bacteria shuttle vector

   Expression

   Bacterial

   Available Since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-ccdB-3xFLAG-V5

    Plasmid

    #87063

    Purpose

    Mammalian Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

    Depositor

    Mikko Taipale, Susan Lindquist

    Article

    Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

    Type

    Empty backbone

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Available Since

    March 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR1A-GFP-N2 (FR1)

    Plasmid

    #19364

    Purpose

    Gateway entry vector for C-term eGFP fusion, frame N2

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Gateway entry vector

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDCC6

    Plasmid

    #59985

    Purpose

    Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

    Depositor

    Peter Duchek

    Article

    Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

    Inserts

    Cas9
    U6-2>sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG

    Promoter

    U6-96Ab and hsp70Bb

    Available Since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG_smFP Myc

    Plasmid

    #59757

    Purpose

    encodes multiple copies of an epitope tag Myc

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    smFP _Myc
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCV-OPA1

    Plasmid

    #26047

    Depositor

    David Chan

    Article

    Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

    Insert

    OPA1 (OPA1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC0040-LwaCas13a crRNA backbone

    Plasmid

    #103851

    Purpose

    Backbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAA

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    U6-LwaCas13aDR-BbsI-BbsI-polyT
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRXIP GFP-Stx17 WT

    Plasmid

    #45909

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    Syntaxin 17 (STX17 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Promoter

    CMV

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. multi-shRNA vector

    Plasmid

    #12391

    Depositor

    Zuoshang Xu

    Article

    Xia et al Biotechniques. 2006 Jul . 41(1):64-8.

    Insert

    mSOD2 shRNA (Sod2 Mouse)
    

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-Ub KO.G76V

    Plasmid

    #11932

    Depositor

    Nico Dantuma

    Article

    Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

    Insert

    Ubiquitin KO.G76V (RBX1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Conjugation deficient mutant. All 7 lysines of ub…

    Available Since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBabe puro HA PIK3CA

    Plasmid

    #12522

    Purpose

    Retroviral expression of HA tagged phosphoinositide 3-kinase

    Depositor

    Jean Zhao

    Article

    Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Aug. 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. MIP 247 CoMiP 4in1 with shRNA p53

    Plasmid

    #63726

    Purpose

    To express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).

    Depositor

    Mark Kay, Joseph Wu

    Article

    Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103

    Insert

    Mini-intronic-plasmid intron (POU5F1, KLF4, SOX2, MYC, TP53 Human)
    

    Tags

    IRES-dtomato

    Expression

    Mammalian

    Mutation

    codon-optimised

    Promoter

    SFFV for Pct4, Klf4, Sox2 and c-myc; U6 for p53

    Available Since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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