19,634 results

1. pEGFP-Q74

   Plasmid

   #40262

   Depositor

   David Rubinsztein

   Article

   Narain et al J Med Genet. 1999 Oct;36(10):739-46.

   Insert

   HTT partial exon 1 Q74 (HTT Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   expanded poly-Q

   Promoter

   CMV

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-HIF1alpha P402A/P564A-pcDNA3

   Plasmid

   #18955

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 1 alpha (HIF1A Human)
   

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids: Proline 402 to Alanin…

   Available Since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3xHA-TurboID_pAS31

   Plasmid

   #118220

   Purpose

   expresses 3xHA-tagged TurboID in C. elegans intestine

   Depositor

   Jessica Feldman

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Tags

   3x HA tag

   Expression

   Worm

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   ges-1p

   Available Since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-mCherry-CDK4KTR

   Plasmid

   #126680

   Purpose

   Fluorescent reporter for CDK4/6 activity

   Depositor

   Hee Won Yang

   Article

   Yang et al Elife. 2020 Apr 7;9. pii: 44571. doi: 10

   Insert

   RB cterm
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCHA-VRC01-scFv

   Plasmid

   #44874

   Depositor

   Dane Wittrup

   Article

   Mata-Fink et al J Mol Biol. 2013 Jan 23;425(2):444-56. d

   Insert

   VRC01 scFv
   

   Tags

   HA and Myc

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. scFLARE2

   Plasmid

   #158700

   Purpose

   Neuronal expression of scFLARE2 component

   Depositor

   Alice Ting

   Article

   Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

   Insert

   scFLARE2
   

   Use

   AAV

   Tags

   HA, Flag, VP16

   Expression

   Mammalian

   Mutation

   Truncated form of TEV protease (220-242 amino aci…

   Promoter

   Syn

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHN924

   Plasmid

   #178885

   Purpose

   Expression of GST-His8-HRV3C

   Depositor

   Kazuhiro Abe

   Insert

   human rhinovirus 3C protease
   

   Tags

   GST, His8

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-Tubulin-6

   Plasmid

   #56450

   Purpose

   Localization: Microtubules, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Tubulin (TUBA1B Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. His6-pAG-RTase

   Plasmid

   #214932

   Purpose

   Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems

   Depositor

   Chuan He

   Article

   Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s

   Insert

   fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…

   Promoter

   T7 promoter

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. ND6-DdCBE-left side TALE

    Plasmid

    #157842

    Purpose

    expresses left-side TALE–DddAtox half of ND6-DdCBE in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    SOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–SOD2 3'UTR
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTRIP-GFP-IRF3

    Plasmid

    #127663

    Purpose

    Over-express EGFP-IRF3

    Depositor

    Nicolas Manel

    Article

    Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

    Insert

    IRF3 (IRF3 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO5.sgRNA.EFS.tRFP

    Plasmid

    #57823

    Purpose

    3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    tagRFP
    

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBHRSF184

    Plasmid

    #89482

    Purpose

    Express asparaginyl endopeptidase 1 from O. affinis (OaAEP1)

    Depositor

    Hideo Iwai

    Article

    Mikula et al FEBS Lett. 2017 Mar 30. doi: 10.1002/187

    Insert

    Asparaginyl endopeptidase from Oldenlandia affinis
    

    Tags

    Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag-GFP-TurboID-NES_pLX304

    Plasmid

    #202019

    Purpose

    expresses GFP-TurboID in the mammalian cytosol, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    Flag-GFP-TurboID-NES
    

    Use

    Lentiviral

    Tags

    Flag and GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmScarlet3-Giantin_C1

    Plasmid

    #189773

    Purpose

    Construct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusion

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3-Giantin
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT3-EF1aH NICD1

    Plasmid

    #86500

    Purpose

    Mammalian expression of Myc tagged NICD1

    Depositor

    Xin Chen

    Article

    Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

    Insert

    Notch1 (C-term) (Notch1 Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Cytoplasmic domain only (AA 1753-2531)

    Promoter

    EF1a

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. ND6-DdCBE-right side TALE

    Plasmid

    #157841

    Purpose

    expresses right-side TALE–DddAtox half of ND6-DdCBE in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    COX8A MTS–3xFLAG–ND6 right TALE–G1397 DddA-C–UGI–ATP5B 3'UTR
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. MIGR1

    Plasmid

    #27490

    Depositor

    Warren Pear

    Article

    Pear et al Blood. 1998 Nov 15. 92(10):3780-92.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP and IRES

    Expression

    Mammalian

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET26-MBP-GLIC

    Plasmid

    #20887

    Depositor

    Raimund Dutzler

    Article

    Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

    Insert

    pentameric ligand gated ion channel from Gloeobacter violaceus PCC 7421
    

    Tags

    His, MBP, and pelB

    Expression

    Bacterial

    Available Since

    Oct. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid

    #118154

    Purpose

    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-P2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    EF1a core and U6

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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