178,652 results

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pcDNA3.1/opto-b2AR-EYFP

Plasmid

#20948

Purpose

Mammalian expression of opto-b2AR fused to EYFP for optical control of GPCR signaling

Depositor

Karl Deisseroth

Publication

Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

opto-b2AR

Expression

Mammalian

Available since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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shCD44-2 pRRL

Plasmid

#19123

Depositor

Bob Weinberg

Publication

Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Insert

small hairpin RNA against CD44 (CD44 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Oct. 2, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET11phiC31poly(A)

Plasmid

#18942

Depositor

Michele Calos

Publication

Groth et al Genetics. 2004 Apr . 166(4):1775-82.

Insert

phiC31 integrase (int Streptomyces phage phiC31)

Expression

Bacterial

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PIK3CA - Exon20 H1047R

Plasmid

#16639

Depositor

Bert Vogelstein

Publication

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PIK3CA H1047R (PIK3CA Human)

Tags

Flag

Expression

Mammalian

Mutation

H1047R

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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CD3 delta YFP

Plasmid

#11951

Depositor

Nico Dantuma

Publication

Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CD3delta-YFP

Expression

Mammalian

Available since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDY0181 Cargo EGFP with AttP Bxb1 site (Parent minicircle)

Plasmid

#179115

Purpose

Cargo EGFP with AttP Bxb1 site (Parent minicircle)

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

Cargo EGFP with AttP Bxb1 site

Use

CRISPR

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSOUP

Plasmid

#165419

Purpose

The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Publication

Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

Insert

Rep A

Use

Helper plasmid required for replication for all p…

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-hSyn-OsTIR1(F74G)

Plasmid

#140730

Purpose

OsTIR1(F74G)-P2A-mAID-EGFP-NES

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74G)-P2A-mAID-EGFP-NES

Use

AAV

Expression

Mammalian

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET-28b-RfxCas13d-His

Plasmid

#141322

Purpose

Plasmid for bacterial expression and purification of RfxCas13d protein

Depositor

Ariel Bazzini, Miguel Angel Moreno-Mateos

Publication

Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

Insert

Rfx-Cas13d

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

Plasmid

#140002

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Publication

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

Promoter

CMV and T7

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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ER-mNeonGreen

Plasmid

#137804

Purpose

Visualization of the Endoplasmic Reticulum

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

KDEL

Expression

Mammalian

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pAG153 FKBP-CFAST11

Plasmid

#130813

Purpose

Expresses FKBP-CFAST11 in mammalian cells

Depositor

Arnaud Gautier

Publication

Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FKBP-CFAST11

Tags

Myc-tag

Expression

Mammalian

Promoter

CMV

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)

Plasmid

#128604

Purpose

Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

Depositor

Marvin Tanenbaum

Publication

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Inserts

24xMoonTag
kinesin family member 18b (KIF18B Human)

Expression

Mammalian

Promoter

Pcmv

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Mm-PylRS-AF/Pyl-tRNACUA

Plasmid

#122650

Purpose

tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.

Depositor

Howard Hang

Publication

Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

Insert

PylRS-AF, Pyl-tRNACUA

Tags

FLAG

Expression

Mammalian

Mutation

Tyrosine 306 changed to Alanine, Tyrosine 384 cha…

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCDNA3.1-pCMV-PfV-GS-Sapphire

Plasmid

#116933

Purpose

Mammalian vector for expression of the 40nm GEM

Depositor

Liam Holt

Publication

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

Pfv

Tags

Sapphire

Expression

Mammalian

Promoter

CMV

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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AAV CMV-dSaCas9-KRAB-bGHpA

Plasmid

#106219

Purpose

AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

Depositor

Charles Gersbach

Publication

Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

Insert

dead S. aureus Cas9 KRAB

Use

AAV and CRISPR

Tags

HA-NLS

Expression

Mammalian

Mutation

D10A and N580A

Promoter

CMV

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEGFP-VSP4-E228Q

Plasmid

#80351

Purpose

Expression of GFP-tagged, dominant negative VPS4 mutant

Depositor

Wesley Sundquist

Publication

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VPS4-E228Q (VPS4A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pKIR1.1

Plasmid

#85758

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter

Depositor

Tetsuya Higashiyama

Publication

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG

Expression

Plant

Promoter

AtRPS5A

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pY109 (lenti-LbCpf1)

Plasmid

#84740

Purpose

Lenti virus delivery of LbCpf1 and crRNA guide

Depositor

Feng Zhang

Publication

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huLbCpf1
puromycin resistance gene

Use

Lentiviral

Tags

3xHA, NLS, and P2A

Promoter

EFS

Available since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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mouse E-cadherin GFP (423)

Plasmid

#67937

Purpose

Mammalian expression of E-cadherin-GFP

Depositor

Alpha Yap

Publication

Truffi et al J Cell Sci. 2014 Jun 1;127(Pt 11):2420-3

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

E-cadherin (Cdh1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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