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pEFIRES-P-mTagBFP-KDEL

Plasmid

#87163

Purpose

Fluorescent marker of endoplasmic reticulum (ER)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

mTagBFP-KDEL

Tags

Bip signal peptide (Homo sapiens) and mTagBFP-KDE…

Expression

Mammalian

Promoter

EF-1 alpha

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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CROPseq-3P5P

Plasmid

#219680

Purpose

CROPseq vector enabling gRNA detection via mRNA-based capture (CROPseq), 3'-based direct capture using 10x capture sequence 1 or 5'-based enrichment using a custom reverse transcription primer

Depositor

Christoph Bock

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4-GFP-Vinculin

Plasmid

#107153

Purpose

mammalian expression

Depositor

Tina Izard

Article

Chinthalapudi et al J Cell Biol. 2014 Dec 8;207(5):643-56. d

Insert

Vinculin (VCL Human)

Tags

(His)10-GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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TRX1 His C32S C35S

Plasmid

#21286

Depositor

Wang Min

Article

Liu et al Circ Res. 2002 Jun 28. 90(12):1259-66.

Insert

TRX1 (TXN Human)

Tags

His

Expression

Mammalian

Mutation

C32S C35S

Available Since

July 30, 2009

Availability

Academic Institutions and Nonprofits only
UAS:iGluSnfr.zf1

Plasmid

#108356

Purpose

UAS driven variant of iGluSnFR for zebrafish expression

Depositor

Harold Burgess

Article

Tabor et al Curr Biol. 2018 Aug 20;28(16):2527-2535.

Insert

UAS:[GlyRa1 secretion sequence]-[iGluSnFR]

Use

Zebrafish expression

Promoter

lac

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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NeonGreen-C1-ORP2

Plasmid

#220069

Purpose

overexpresses ORP2 with a neonGreen fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

OSBPL2 (OSBPL2 Human)

Expression

Mammalian

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-PIK3R1

Plasmid

#174191

Purpose

Expresses PIK3R1 in mammalian cells

Depositor

Cheng-han Yu

Article

Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

Insert

PIK3R1 (PIK3R1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-C1-AKT-PH

Plasmid

#21218

Depositor

Tobias Meyer

Article

Raucher et al Cell. 2000 Jan 21. 100(2):221-8.

Insert

AKT-PH

Tags

GFP

Expression

Mammalian

Mutation

PH domain of AKT. (Lab plasmid WC 0002).

Available Since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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pMT_025

Plasmid

#158579

Purpose

Lentiviral expression of a customizable open reading frame

Depositor

John Doench, David Root

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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FU - dio YFP - W

Plasmid

#73858

Purpose

For expressing YFP in mammalian cells upon Cre co-expression. Can be made into Lentivirus.

Depositor

Elly Nedivi

Article

Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

Insert

eYFP

Use

Lentiviral

Expression

Mammalian

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

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p23-DDX21 WT

Plasmid

#128803

Purpose

overexpression of DDX21 in human cells

Depositor

Ling-Ling Chen

Article

Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

Insert

DDX21 WT

Tags

flag

Expression

Mammalian

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGEP179_pX330K

Plasmid

#137882

Purpose

Entry vector for multi-gRNA cloning

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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NeonGreen-C1-ORP6

Plasmid

#220072

Purpose

overexpresses ORP6 with a neonGreen fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

OSBPL6 (OSBPL6 Human)

Expression

Mammalian

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pXY027

Plasmid

#98915

Purpose

Express FtsZ tagged with GFP in E coli

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

FtsZ

Tags

GFP

Expression

Bacterial

Promoter

T5-lac

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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His SUMO HNRNPA1 dHexa

Plasmid

#197013

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Molliex et al Cell. 2015 Sep 24;163(1):123-33. doi: 10

Insert

HNRNPA1

Tags

6His-SUMO

Expression

Bacterial

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hNox5

Plasmid

#69354

Purpose

expresses human Nox5 in mammalian cells

Depositor

Karl-Heinz Krause

Article

Banfi et al J Biol Chem. 2001 Oct 5;276(40):37594-60

Insert

hNox5 (NOX5 Human)

Tags

no

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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Myc tag WT PTB

Plasmid

#23024

Depositor

Douglas Black

Article

Xie et al Proc Natl Acad Sci U S A. 2003 Jul 22. 1

Insert

PTB1 (PTBP1 Human)

Tags

Myc

Expression

Mammalian

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_SM-FLuc0-pA

Plasmid

#210583

Purpose

Expresses SM(FLAG)-nonoptimal Firefly luciferase- pA

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-nonoptimal FLuc

Use

Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Flag Rab12 Q101L

Plasmid

#86788

Purpose

mammalian expression of Rab12 Q101L, constitutively GTP-bound mutant

Depositor

Peter McPherson

Article

Xu et al EMBO Rep. 2015 Jun;16(6):709-18. doi: 10

Insert

Rab12 (RAB12 Human)

Tags

Flag

Expression

Mammalian

Mutation

Q101L, constitutively GTP-bound mutant

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR2b-mTurquoise2

Plasmid

#191014

Purpose

Gateway entry vector with the mTurquoise2 fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

Insert

mTurquoise2

Use

Gateway entry vector

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGFP-Rab8A[T22N]

Plasmid

#24899

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A[T22N]

Tags

GFP

Expression

Mammalian

Mutation

Changed Threonine 22 to Asparagine

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pGFP-Rab8A[Q67L]

Plasmid

#24900

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A[Q67L]

Tags

GFP

Expression

Mammalian

Mutation

Changed Glutamine 67 to Leucine

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-HA-gate-pGK-HYG

Plasmid

#107396

Purpose

Lentiviral gateway destination vector

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

HA

Expression

Mammalian

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_SUMO_AcCas12n

Plasmid

#203808

Purpose

pET28a-based plasmid containing a T7-driven His6-SUMO-HRV3c-AcCas12n cassette. Kanamycin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

SUMO_AcCas12n

Use

CRISPR

Promoter

T7

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pLG033 (ietAS)

Plasmid

#157911

Purpose

Expresses the ietAS defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

ietAS (ATPase + serine protease)

Expression

Bacterial

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

Plasmid

#74010

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

Depositor

Filippo Del Bene

Article

Di Donato et al Genome Res. 2016 Mar 8.

Inserts

5xUAS
Cas9
Cre
U6 promoter

Use

CRISPR

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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Frb-NLS-mCherry

Plasmid

#217613

Purpose

part B of SPARK-OFF

Depositor

Xiaokun Shu

Article

Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

Insert

Frb-NLS-mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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ALKBH5H204A-dCas9

Plasmid

#134784

Purpose

Expression fusion protein ALKBH5H204A-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Article

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

ALKBH5 and inactive dCas9

Expression

Mammalian

Mutation

H204A

Promoter

CMV

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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SBE-NLS-mCherry-P2A-CreERT2 PGK-rtTA3

Plasmid

#196936

Purpose

TGFbeta reporter driven nuclear mCherry and CreERT2 with PGK driven rtTA3

Depositor

Elaine Fuchs

Article

Oshimori et al Cell. 2015 Feb 26;160(5):963-976. doi: 1

Insert

mCherry

Use

Lentiviral and RNAi

Tags

P2A-CreERT2

Expression

Mammalian

Promoter

SBE

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLK65

Plasmid

#37257

Depositor

Ann Hubbard

Article

Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

Insert

vang-like 2 (VANGL2 Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only