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We narrowed to 7,654 results for: Pol

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Showing: 3141 - 3160 of 7654 results

  1. pET28a(+)::MBP-NL3F10H

    Plasmid
    #141291
    Purpose
    MBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Publication
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    MBP-NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3), His (x10), and MBP
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    141291-plasmid-map-sequence-id-324616

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  3. myc-hRAD18 dC2

    Plasmid
    #68831
    Purpose
    Expresses c-myc-tagged human RAD18 deleting Polymerase eta binding domain
    Depositor
    Satoshi Tateishi
    Publication
    Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    c-myc tagged human RAD18 deleting Polymerase eta binding domain (RAD18 Human)
    Tags
    c-myc
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pB80-hsKIF5B(1-560)-L-GFP

    Plasmid
    #193716
    Purpose
    Expression of GFP tagged human kinesin-1 (KIF5B aa1-560)
    Depositor
    Lukas Kapitein
    Publication
    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hsKIF5B(1-560)-L-GFP (KIF5B Synthetic, Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    hsKIF5B(aa1-560);
    Promoter
    Chicken beta-actin
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    193716-plasmid-map-sequence-id-383721

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  6. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q52

    Plasmid
    #111748
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Publication
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q52 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111748-plasmid-map-sequence-id-220486

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  8. pEGFP-C3-PLK4-S285A/T289A-3xFLAG

    Plasmid
    #69841
    Purpose
    This plasmid encodes PLK4 isoform 1 carrying S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag
    Depositor
    Michel Bornens
    Publication
    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PLK4 (PLK4 Human)
    Tags
    3xFLAG tag and EGFP
    Expression
    Mammalian
    Mutation
    S285A/T289A
    Promoter
    CMV
    Available since
    Dec. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    69841-plasmid-map-sequence-id-123880

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  9. pRJPaph-gusA

    Plasmid
    #67036
    Purpose
    Plasmid for Paph-gusA integration downstream of B. diazoefficiens (B. japonicum) scoI
    Depositor
    Julia Vorholt
    Publication
    Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
    gusA
    Use
    Conjugative bacterial vector
    Available since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67036-plasmid-map-sequence-id-115551

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  10. pGS-CD64-2

    Plasmid
    #109191
    Purpose
    Encodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression system
    Depositor
    Monika Golas
    Publication
    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do
    Insert
    Full-length engineered CD64, derived from human CD64
    Tags
    Twin-Strep
    Expression
    Insect
    Mutation
    engineered for efficient expression, see publicat…
    Promoter
    Polyhedrin
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    109191-plasmid-map-sequence-id-233794

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  11. pFastBac1-pGC-A

    Plasmid
    #186626
    Purpose
    pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)
    Depositor
    Debra Hansen
    Publication
    Zhang et al Sci Rep. 2022 Jul 12;12(1):11824. doi: 1
    Insert
    NPR1 (NPR1 Human)
    Tags
    hemagglutinin (HA) signal peptide + His10-tag + T…
    Expression
    Insect
    Mutation
    deleted amino acids 1-32
    Promoter
    polyhedring
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    186626-plasmid-map-sequence-id-366906

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  12. pOpen-dbh

    Plasmid
    #165513
    Purpose
    Thermostable gamma-family lesion-bypass DNA Polymerase that efficiently synthesizes DNA across a variety of DNA template lesions.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Sulfolobus DNA Polymerase IV
    Use
    Synthetic Biology
    Available since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165513-plasmid-map-sequence-id-338598

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  13. pCfB6684

    Plasmid
    #106154
    Purpose
    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6631, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Publication
    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/
    Type
    Empty backbone
    Expression
    Yeast
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    106154-plasmid-map-sequence-id-206237

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  18. pcDNA3.1-N-Myc_1-205 USP7

    Plasmid
    #131245
    Purpose
    Mammalian expression of the USP7 TRAF-like domain with an N-terminal Myc tag.
    Depositor
    Roger Woodgate
    Publication
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Expresses a mutant form of our pcDNA3.1-N-Myc_1-5…
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131245-plasmid-map-sequence-id-269478

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  20. pST50Trc2-DHFRHIS

    Plasmid
    #63967
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Publication
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Dec. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    63967-plasmid-map-sequence-id-177373

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Showing: 3141 - 3160 of 7654 results