We narrowed to 6,701 results for: proc

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mEmerald(luminal)-canxTM

Plasmid

#186961

Purpose

Expresses mEmerald connected with the N-terminus of Calnexin transmembrane domain.

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

CANX (CANX Human)

Tags

mEmerald

Expression

Mammalian

Mutation

Only express calnexin (482-end) with ER signal pe…

Promoter

CMV Promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMax_mOrange_A215C

Plasmid

#177828

Purpose

Site-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mOrange_A215C

Expression

Mammalian

Mutation

A215C

Promoter

CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-hu-N-Myc

Plasmid

#50772

Purpose

retroviral expression of human N-Myc

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

N-myc (MYCN Human)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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p026 pCS2-EnR

Plasmid

#11028

Depositor

Ramesh Shivdasani

Article

Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

Insert

engrailed (en Fly)

Use

Xenopus, mammalian, avian, and zebrafish

Mutation

Engrailed repressor domain (bases encoding amino …

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pCDNA-mSin1(1.1)-HA

Plasmid

#73388

Purpose

Expresses mSin-HA

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

mSin1 transcript variant protein isoform 1 (MAPKAP1 Human)

Tags

HA tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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[pSK24] pACYCDuet His-c7orf59|HBXIP

Plasmid

#136143

Purpose

bacterial expression of c7orf59 and HBXIP

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

Insert

c7orf59 and HBXIP (LAMTOR4, LAMTOR5 Human)

Expression

Bacterial

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pTMiniTrex-mCherry-Shark1

Plasmid

#233143

Purpose

Serves as a template plasmid for amplifying Shark1 (Tet-OFF) hammerhead aptazyme-3xTy-tagging cassette

Depositor

Ronald Etheridge

Article

Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

Inserts

mCherry
Shark1

Use

Prokaryotic expression

Tags

3x Ty tag

Mutation

Codon optimized for Trypanosoma cruzi and changed…

Available Since

March 25, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pBP-split-intein-Gal4[1-20]-N-int

Plasmid

#199201

Purpose

Gateway destination vector for enhancer-driven split-intein Gal4[1-20]N-int expression

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein-Gal4[1-20]-N-int

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCAMBIA-FLS2-myc-mCherry

Plasmid

#86159

Purpose

FLS2-mCherry expression

Depositor

Silke Robatzek

Article

Mbengue et al Proc Natl Acad Sci U S A. 2016 Sep 27;11

Insert

FLS2 (FLS2 Mustard Weed)

Tags

mCherry and myc

Expression

Plant

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pNubWtXgate

Plasmid

#105079

Purpose

Yeast mating-based Split Ubiquitin Assay, positive control

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

NubI

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pGMCtKq28-TSC10M1-sspB

Plasmid

#49526

Purpose

Kanamycin resistant; contains qtag28, expresses sspB under a promoter containing PtetO, and constutively expresses single-chain TetR10 (tsc10); integrates into the mycobacteriophage att-Tweety site

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

TSC10M1-sspB

Expression

Bacterial

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST-HemmarR

Plasmid

#31222

Depositor

Yuh-Nung Jan

Article

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Insert

CD4-tdTom (CD4 Fly, Aequorea victoria, Human)

Tags

CD4 and tdTomato

Expression

Insect

Mutation

Fusion of signal peptide of Drosophila Akh gene, …

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pUF1-dCas9-GCN5

Plasmid

#122509

Purpose

Combining with the specific sgRNA sequence to activate of the Plasmodium falciparum endogenous gene transcription, through the increasing of H3K9 acetylation.

Depositor

Lubin Jiang

Article

Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

Insert

dCas9-GCN5

Use

CRISPR

Tags

3*FLAG

Mutation

dCas9(D10A,H840A)

Promoter

PfHsp86

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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Stat1 beta Flag pRc/CMV

Plasmid

#8704

Depositor

Jim Darnell

Article

Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

Insert

STAT1 beta (STAT1 Human)

Tags

Flag Tag

Expression

Mammalian

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only
pTRE-Tight-FLAG-CYLD-WT

Plasmid

#60030

Purpose

WT CYLD under control of Tet promoter with N-terminal FLAG tag

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Tags

FLAG

Expression

Mammalian

Promoter

TRE

Available Since

Nov. 25, 2014

Availability

Academic Institutions and Nonprofits only
pMax_GFP_C289T

Plasmid

#177826

Purpose

Site-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

GFP_C289T

Expression

Mammalian

Mutation

C289T

Promoter

CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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E42_gRunx1_gRNA3_dTet_NGFR

Plasmid

#189803

Purpose

Retroviral delivery of guide RNA against mouse Runx1

Depositor

Ellen Rothenberg

Article

Shin et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

gRunx1_gRNA3 (Runx1 Mouse)

Use

Retroviral

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV14_NOP58

Plasmid

#73063

Purpose

expression clone for human NOP58 with C-terminal 3xFLAG-tag

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

NOP58 ribonucleoprotein (NOP58 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-M2-TLR2

Plasmid

#12291

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I and H202Y; signal peptide (aa1-23) replaced …

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
Stat1 alpha pRC/CMV

Plasmid

#8690

Depositor

Jim Darnell

Article

Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

Insert

STAT1 alpha (STAT1 Human)

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only