We narrowed to 1,041 results for: Psp

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pSP64T-ythdf1-3xflag

Plasmid

#164488

Purpose

Expresses zebrafish ythdf1 with 3x flag tag, for mRNA injection

Depositor

Antonio Giraldez

Article

Kontur et al Cell Rep. 2020 Dec 29;33(13):108598. doi

Insert

ythdf1 (ythdf1 Zebrafish)

Use

Production of mrna for zebrafish injection.

Tags

3x-flagtag

Promoter

SP6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSP64T-ythdf3-3xflag

Plasmid

#164490

Purpose

Expresses zebrafish ythdf3 with 3x flag tag, for mRNA injection

Depositor

Antonio Giraldez

Article

Kontur et al Cell Rep. 2020 Dec 29;33(13):108598. doi

Insert

ythdf3 (ythdf3 Zebrafish)

Use

Production of mrna for zebrafish injection.

Tags

3x-flagtag

Promoter

SP6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSP64-hERG1a D540K

Plasmid

#53057

Purpose

Expresses alpha subunit of D540K hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Sanguinetti et al J Physiol. 1999 Feb 1;514 ( Pt 3):667-75

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Mutation

changed Aspartate 540 to Lysine

Promoter

SP6

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pSP64-hERG1a S620T

Plasmid

#53059

Purpose

Expresses alpha subunit of S620T hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Casis et al Mol Pharmacol. 2006 Feb;69(2):658-65. Ep

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Mutation

changed Serine 620 to Threonine

Promoter

SP6

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pSP64-hERG1a S631A

Plasmid

#53058

Purpose

Expresses alpha subunit of S631A hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Zou et al J Physiol. 1998 May 15;509 ( Pt 1):129-3

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Mutation

changed Serine 631 to Alanine

Promoter

SP6

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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HOXB4-FLAG-pSP65

Plasmid

#20990

Depositor

Corey Largman

Article

Chang et al Genes Dev. 1995 Mar 15. 9(6):663-74.

Insert

HOXB4 (HOXB4 Human)

Use

In vitro trans/trans

Mutation

Missing first 14 amino acids

Available Since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
HOXB6-FLAGA-pSP65

Plasmid

#20994

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res. 1996 Mar 1;24(5):898-

Insert

HOXB6 (HOXB6 Human)

Use

In vitro trans/trans

Tags

FLAG

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
ZE49 pSPORT1-Sp5-l

Plasmid

#17090

Depositor

Randall Moon

Insert

Sp5l (sp5l Zebrafish)

Use

Zebrafish expression

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pSP64T XAR1 (DM#105)

Plasmid

#15044

Depositor

Douglas Melton

Article

Hemmati-Brivanlou et al Nature. 1992 Oct 15. 359(6396):609-14.

Insert

Xenopus activin receptor 1 (xstk2 Frog)

Expression

Bacterial

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pSP70 Vg1 (DM#139)

Plasmid

#15070

Depositor

Douglas Melton

Article

Tannahill et al Development. 1989 Aug . 106(4):775-85.

Insert

Vg1 (gdf1 Frog)

Expression

Bacterial

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pSP64TNE Xnr4 (DM#174)

Plasmid

#15078

Depositor

Douglas Melton

Article

Joseph et al Dev Biol. 1997 Apr 15. 184(2):367-72.

Insert

Xnr4 (xnr4 Frog)

Expression

Bacterial

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

Plasmid

#132560

Purpose

Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.

Depositor

Baisong Lu

Article

Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

Inserts

ABEmax
sgRNA, with Tetraloop replaced by com aptamer

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pspCas9-ABE-3'UTR-sgRNA-ST2-com vector

Plasmid

#136270

Purpose

Expresses ABE and ST2-com modified sgRNA scaffold

Depositor

Baisong Lu

Article

Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

Insert

ABE and sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-PspCas13b-non-targeting (pJK5)

Plasmid

#229052

Purpose

Cloning backbone for PspCas13b crRNA (U6 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

Plasmid

#221552

Purpose

Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

gRNA targeting human Rab7A (RAB7A Human)

Expression

Mammalian

Promoter

U6

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMJ127: pRS426-His-MBP-NlovFz2_PSP1

Plasmid

#205257

Purpose

S. cerevisiae expression vector for NlovFz2 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2

Tags

10xHis, MBP

Expression

Yeast

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA

Plasmid

#164471

Purpose

Expresses sgRNA targeting human GJA1 locus

Depositor

Markus Kuehn

Article

Cheng et al Stem Cells. 2022 Mar 9. pii: 6545973. do

Insert

Gap junction alpha-1 (GJA1) (GJA1 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B2

Plasmid

#165083

Purpose

gRNA 2 of pair B for Stx5L-specific knockout

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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Helios-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75244

Purpose

CRISPR/Cas9 plasmid against human Helios

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Helios (IKZF2 Human)

Use

CRISPR

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSP303 pRS306 pRPS2-Far1DS-NLS-mCherry

Plasmid

#68279

Purpose

SKARS: Fus3 MAPK activity sensor: Far1DS 2xNLS mCherry

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Far1DS NLS mCherry

Tags

mCherry

Expression

Yeast

Promoter

RPS2

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)

Plasmid

#218513

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferase

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Expression

Bacterial

Mutation

deletion of the Plac_mrfp cassette

Promoter

PgyrA_ermBL-ermB'

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9-GFP UGT2 (pWZ587)

Plasmid

#254038

Purpose

Express SpCas9 and the UGT2 gRNA

Depositor

Jef Boeke

Article

Barriball et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

gRNA_UGT2

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-puro-mAnkrd11 (intron 4)

Plasmid

#236609

Purpose

Knockouts Ankrd11 in mouse cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

Ankrd11 (Ankrd11 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-puro-hANKRD11 (exon 5-2)

Plasmid

#236613

Purpose

Knockouts ANKRD11 in human cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pSplit-SWITCHER1-TF1-dsRED

Plasmid

#213044

Purpose

A CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassette

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

Split-SWITCHER1-TF1-dsRED

Use

CRISPR

Expression

Mammalian

Promoter

cBH

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pSplit-SWITCHER2-TF1-dsRED

Plasmid

#213045

Purpose

A CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassette

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

Split-SWITCHER1-TF1-dsRED

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-puro-hANKRD11 (exon 5-1)

Plasmid

#236612

Purpose

Knockouts ANKRD11 in human cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Use

CRISPR

Expression

Mammalian

Available Since

June 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-puro-mAnkrd11 (intron 3)

Plasmid

#236608

Purpose

Knockouts Ankrd11 in mouse cells

Depositor

Tadashi Nakagawa

Article

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

Ankrd11 (Ankrd11 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP267: pHelper(CyCAST)_PSP1_ΔTniQ

Plasmid

#200842

Purpose

Inducible expression of CyCAST proteins (except TniQ) with crRNA targeting CyPSP1.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP269: pHelper(CyCAST)_PSP1_ΔTnsD

Plasmid

#200844

Purpose

Inducible expression of CyCAST proteins (except TnsD) with crRNA targeting CyPSP1.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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