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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 2,847 results for: PHI-1

Showing: 751 - 800 of 2847 results

  27. pMT-FLAG-CBPΔN

    Plasmid
    #182840
    Purpose
    A 5.2 kb Drosophila CBP cDNA fragment (cut by Nhe I and Nsi I), encoding C-terminal residues 1476-3222, was inserted into the pMT-FLAG vector. Expression in S2 cells by CuSO4 induction.
    Depositor
    Peter Harte
    Article
    Tie et al Development. 2009 Sep;136(18):3131-41. d
    Insert
    CBP cDNA fragment (cut by Nhe I and Nsi I) (nej Fly)
    Use
    Tags
    FLAG tag
    Expression
    Insect
    Mutation
    Promoter
    MT
    Available since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    182840-plasmid-map-sequence-id-360048

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  35. pCAGGS-Twin-Strep-TEV-DGKζ

    Plasmid
    #202531
    Purpose
    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human diacylglycerol kinase ζ (DGKζ) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Insert
    DGKZ (DGKZ Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and chicken β-actin promoter
    Available since
    July 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    202531-plasmid-map-sequence-id-399624

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  36. pnEA-NpG-DmCaf40_25-291_R

    Plasmid
    #147354
    Purpose
    Bacterial Expression of DmCaf40_25-291
    Depositor
    Elisa Izaurralde
    Insert
    DmCaf40_25-291 (Rcd-1 Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147354-plasmid-map-sequence-id-289801

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  37. pBS NL4-3 envFS eGFP 5'CMV Δtat

    Plasmid
    #101347
    Purpose
    tat ATG->ACG (silent in vpr reading frame), nt 78 mutated T->G to change Tyr to stop codon, nt 116 mutated T->C to disrupt Met, 5'LTR replaced with CMV-R-U5 from pALPS for tat-independent transcription in HEK293E cells.
    Depositor
    Jeremy Luban
    Article
    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:
    Insert
    NL4-3
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    delta Tat
    Promoter
    Available since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    101347-plasmid-map-sequence-id-194257

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  42. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA

    Plasmid
    #136652
    Purpose
    Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD-9
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136652-plasmid-map-sequence-id-273328

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  43. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

    Plasmid
    #136659
    Purpose
    Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD9:TH1477 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136659-plasmid-map-sequence-id-273359

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  44. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMG18311_2xUGI_3xHA

    Plasmid
    #136657
    Purpose
    Expresses St1BE4max-LMG18311 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD9:LMG18311 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136657-plasmid-map-sequence-id-273344

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  45. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_CNRZ1066_2xUGI_3xHA

    Plasmid
    #136654
    Purpose
    Expresses St1BE4max-CNRZ1066 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD9:CNRZ1066 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136654-plasmid-map-sequence-id-273340

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  48. pG1AK-PheB

    Plasmid
    #71738
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Insert
    PheB
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pRplsWT
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71738-plasmid-map-sequence-id-135061

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Showing: 751 - 800 of 2847 results