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Plasmid#203570PurposeModule for the expression of geneticin resistance and Nanoluciferase, and the expression of firefly luciferase under the Ppaf promoterDepositorInsertPgpdA:Nluc:Ttub::PtrpC:nptII:Ttub::Ppaf:Luc:Ttub
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Camk2b
Plasmid#186819PurposeFluorescent reporter for Camk2b expressionDepositorInsertCamk2b promoter (Camk2b Mouse)
UseLuciferaseTagsExpressionMutationPromoterCamk2b promoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Camk2d
Plasmid#186820PurposeFluorescent reporter for Camk2d expressionDepositorInsertCamk2d promoter (Camk2d Mouse)
UseLuciferaseTagsExpressionMutationPromoterCamk2d promoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGl3-Camk2g
Plasmid#186821PurposeFluorescent reporter for Camk2g expressionDepositorInsertCamk2g promoter (Camk2g Mouse)
UseLuciferaseTagsExpressionMutationPromoterCamk2g promoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRBad-Z-C-DiB-RM
Plasmid#168477PurposeC-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168475: pET11a-Z-N-DiB-RM to obtain DiB-RM‐split-Zip proteinDepositorInsertLeucine Zipper + C-fragment of DiB-RM
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11a-Z-N-DiB-RM
Plasmid#168475PurposeN-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168477: pMRBad-Z-C-DiB-RM to obtain DiB-RM‐split-Zip proteinDepositorInsertN-fragment of DiB-RM + Leucine Zipper
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBW106
Plasmid#111138PurposeFor the expression of rat AANAT with Met-Ser-Ile N-terminus in S. cerevisiaeDepositorInsertMSI-ratAANAT-3FLAG (Leu2 deletion) (Aanat Rat)
UseTags3x FlagExpressionYeastMutationLeu2 deletionPromoterGal1Available sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pCDH_Str-KDEL_ST-SBP-EGFP
Plasmid#65267Purposesynchronize trafficking of ST from the ER (RUSH system)DepositorInsertStr-KDEL and truncated sialyltransferase (ST6GAL1 Human)
UseLentiviralTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
DHB-mVenus
Plasmid#136461PurposeFluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2DepositorInsertDNA Helicase B (HELB Human)
UseLentiviralTagsmVenusExpressionMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX307-VHL(C162F)-3F-BirA
Plasmid#220149PurposeExpresses VHL(C162F)-BirA as a control for VHL substrate identification by E-STUBDepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationchanged cysteine 162 to phenylalanine; codon opti…PromoterEF-1aAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304-VHL-IRES-GFP
Plasmid#187641PurposeVHL Expression vector with GFPDepositorInsertVHL (VHL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV6-mPER2-luc
Plasmid#212035PurposeExpression of murine Per2-luciferase in mammalian cellsDepositorInsertmPer2-luciferase (Per2 Mouse)
UseLentiviral and LuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-myc-his-BACH1 WT
Plasmid#17642DepositorInsertBACH1 (BRIP1 Human)
UseTagsHis and MycExpressionMammalianMutationPromoterAvailable sinceApril 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1-Full-Length-MePCE
Plasmid#113539PurposeProduces an N-terminal GST-tagged full length MePCE protein.DepositorInsertMePCE (MEPCE Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pSMP-EZH2_1
Plasmid#36387DepositorInsertEZH2 (EZH2 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
Brm WT-Flag pShuttle
Plasmid#122105PurposeExpression of full length Brm in mammalian cellsDepositorInsertBRM (SMARCA2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX307-VHL(Y98H)-3F-BirA
Plasmid#220148PurposeExpresses VHL(Y98H)-BirA as a control for VHL substrate identification by E-STUBDepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationchanged tyrosine 98 to histidine; codon optimizedPromoterEF-1aAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mito-CanlonicSF/pCMV-myc-mito
Plasmid#224465PurposeTo explore mitochondrial lactate dynamicsDepositorInsertCanlonicSF
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3 Flag Erg
Plasmid#66977PurposeExpression plasmid for mouse Erg; adds Flag tag to the N-terminus of proteinDepositorInsertErg (Erg Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3 basic Mut E2
Plasmid#48748PurposeExpression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1)DepositorInsertmPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
UseLuciferaseTagsExpressionMutationMutated E-box2 sequence from CACGTT to GCTAGTPromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSMP-EZH2_2
Plasmid#36388DepositorInsertEZH2 (EZH2 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSMP-EZH2_3
Plasmid#36389DepositorInsertEZH2 (EZH2 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLX304-VHL-IRES-Tdtomato
Plasmid#187643PurposeVHL Expression vector with TdtomatoDepositorInsertVHL (VHL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV10-Flag-Fbxl21
Plasmid#48752PurposeExpression of FLAG-tagged Fbxl21DepositorInsertFbxl21 (Fbxl21 Mouse)
UseTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Str-STIM1-NN_ST-SBP-EGFP
Plasmid#65262Purposesynchronize trafficking of ST from the ER (RUSH system)DepositorInsertStr-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Per2:iLuc
Plasmid#160293Purposetarget Per2 gene with a color-switching luciferase cassette in mouse genome and the transfection of the recombinant DNA will lead genomic recombination and make a knock-in allele in mouse ES cells.DepositorInsertPer2LUC
UseTagsExpressionMammalianMutationPromoterno promotorAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-P(Per2)-DIO-mCherry-Venus-NLS-D2
Plasmid#110058PurposePer2 transcription fluorescence reporterDepositorInsertPer2 promoter and mCherry and Venus (Per2 Mouse)
UseAAV and Cre/LoxTagsNLS-D2ExpressionMammalianMutationPromoterPer2Available sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQCXIH BRGI-Flag
Plasmid#19148DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pL6-H2B-iRFP720-puro
Plasmid#189992PurposeLentivirial plasmid for nuclear iRFP-720 expression with puromycin resistanceDepositorInsertHuman histone H2B
UseLentiviralTagsiRFP720ExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM5082
Plasmid#85121PurposePkaiB-kaiC-ecfp expressed from NSII-Gm vector constructed by seamless assembly. Fusion expressed as full length fusion protein at WT levels but is 5hr long period. Used only for co-localization expDepositorInsertPkaiB-KaiC-ECFP
UseTagsECFPExpressionBacterialMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FLAG-BMAL1-S513A/S515A/S516A
Plasmid#186835PurposeExpresses FLAG-BMAL1-S513A/S515A/S516A in mammalian cellsDepositorInsertbmal1 (Bmal1 Mouse)
UseTagsFLAGExpressionMammalianMutationchanged Serine 513/515/516 to AlaninePromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM5085
Plasmid#85122PurposePkaiA-kaiA-ecfp expressed from NSI-SpSm vector constructed by seamless assembly. Full length fusion protein expressed at WT levels. ~2hr long period.DepositorInsertPkaiA-kaiA-ecfp expressed from NSI vector with SpSm resistance constructed by seamless assembly.
UseTagsECFPExpressionBacterialMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pGL3 Pro Mut E2
Plasmid#48750PurposeExpression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)DepositorInsertmPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
UseLuciferaseTagsExpressionMutationMutated E-box2 sequence from CACGTT to GCTAGTPromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSMP-EZH1_1
Plasmid#36359DepositorInsertEZH1 (EZH1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
Str-STIM1-NN_ST-SBP-mCherry
Plasmid#65263Purposesynchronize trafficking of ST from the ER (RUSH system)DepositorInsertStr-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationcontains only amino acids 1 to 43 of STPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSMP-Bmi1_1
Plasmid#36352DepositorInsertBmi1 (BMI1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSCT-GAL93-SFPQ
Plasmid#46320DepositorInsertSFPQ (SFPQ Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL Y98H-pRc/CMV
Plasmid#22044DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseTagsHA-tagExpressionMammalianMutationpoint mutant, converting aa 98 from Tyrosine to H…PromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSMP-Bmi1_3
Plasmid#36354DepositorInsertBmi1 (BMI1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSMP-SUZ12_1
Plasmid#36390DepositorInsertSUZ12 (SUZ12 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSMP-EZH1_2
Plasmid#36360DepositorInsertEZH1 (EZH1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSMP-EZH1_3
Plasmid#36361DepositorInsertEZH1 (EZH1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSMP-SUZ12_2
Plasmid#36391DepositorInsertSUZ12 (SUZ12 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUDE735
Plasmid#103024Purposeexpression of a Cpf1 programming crRNA targetting CAN1, HIS4, PDR12 and ADE2 (crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S)DepositorInsertcrCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMP-Bmi1_2
Plasmid#36353DepositorInsertBmi1 (BMI1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSMP-SETDB1_1
Plasmid#36347DepositorInsertSETDB1 (SETDB1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only