We narrowed to 407 results for: SAM activators

1. MTK3_009

   Plasmid

   #123724

   Purpose

   Encodes nuclear localized PHYB fused to activating domain of VP16 as Type 3 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   PHYB-VP16-NLS
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. MTK3_020

   Plasmid

   #123735

   Purpose

   Encodes rapamycin and tamoxifen activated split-dCAS9 (C-terminal) as a Type 3 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   ERT2-fkbp-spdCa9(205-1368)
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. MTK3_001

   Plasmid

   #123952

   Purpose

   Encodes nuclear localized PHR domain of CRY2 fused to activating domain of p65 as Type 3 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   3XNLS::CRY2PHR::p65
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. MTK3_018

   Plasmid

   #123733

   Purpose

   Encodes rapamycin and tamoxifen activated split-dCAS9 (N-terminal) as a Type 3 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   spdCas9(1-204)-frb-D10A-ERT2
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMax_mOrange_G299C

   Plasmid

   #177829

   Purpose

   Site-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).

   Depositor

   Zachary Nagel, Leona Samson

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   mOrange_G299C
   

   Expression

   Mammalian

   Mutation

   G299C

   Promoter

   CMV

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMax_mOrange_A215C

   Plasmid

   #177828

   Purpose

   Site-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).

   Depositor

   Zachary Nagel, Leona Samson

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   mOrange_A215C
   

   Expression

   Mammalian

   Mutation

   A215C

   Promoter

   CMV

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMax_GFP_C289T

   Plasmid

   #177826

   Purpose

   Site-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).

   Depositor

   Zachary Nagel, Leona Samson

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   GFP_C289T
   

   Expression

   Mammalian

   Mutation

   C289T

   Promoter

   CMV

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMax_BFP_A191G

   Plasmid

   #177823

   Purpose

   Site-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).

   Depositor

   Zachary Nagel, Leona Samson

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   tagBFP_A191G
   

   Expression

   Mammalian

   Mutation

   A191G

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMax_ScaI_BFP

   Plasmid

   #177824

   Purpose

   Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).

   Depositor

   Zachary Nagel, Leona Samson

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   tagBFP
   

   Expression

   Mammalian

   Mutation

   5' inserted ScaI RE cut site

   Promoter

   CMV

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMax_mPlum_C207G/T208C

    Plasmid

    #177831

    Purpose

    Site-specific mutagenesis of mPlum for the purposes of creating a site-specific O6-MeG lesion for the purposes of measuring MGMT activity via Host Cell Reactivation (HCR).

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    mPlum_C207G/T208C
    

    Expression

    Mammalian

    Mutation

    C207G + T208C

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHES839

    Plasmid

    #87941

    Purpose

    Constitutively expresses estradiol-inducible synthetic transcriptional regulator in yeast. Transcriptional regulator activates transcription of promoters containing Gal4 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    GAL4 DBD - hER LBD - MSN2 AD (ESR1 Synthetic, Human, Budding Yeast)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    ADH1 (crippled)

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHES795

    Plasmid

    #87943

    Purpose

    Constitutively expresses estradiol-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    Zif268 DBD - hER LBD - MSN2 AD (ESR1 Synthetic, Human, Budding Yeast)
    

    Expression

    Yeast

    Promoter

    ADH1 (crippled)

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMars1CyeGFP

    Plasmid

    #75222

    Purpose

    Mammalian intracellular expression of fluorogen activating protein Mars1Cy-eGFP fusion

    Depositor

    Alan Waggoner

    Article

    Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

    Insert

    Mars1Cy-EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pP2AeGFP-Mars1Cy

    Plasmid

    #75223

    Purpose

    Mammalian intracellular expression of fluorogen activating protein Mars1Cy and eGFP

    Depositor

    Alan Waggoner

    Article

    Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

    Insert

    EGFP-p2a-Mars1Cy
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. ARB367

    Plasmid

    #124050

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to VPR domain P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::VPR-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti-sgRNA(MS2)_zeo_hCYP3A4_enhancer_guide1

    Plasmid

    #176839

    Purpose

    To recruit SAM complex to human CYP3A4 proximal enhancer.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

    Insert

    Human CYP3A4 proximal enhancer sgRNA for activation
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-OptoFGFR1-D387A

    Plasmid

    #59778

    Purpose

    Expresses optoFGFR1 with mutation in PHR to suppress homointeraction, thus cannot be activated by light

    Depositor

    Won Do Heo

    Article

    Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

    Insert

    OptoFGFR1-D387A (FGFR1 Mustard Weed, Human)
    

    Tags

    mCitrine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAN2981

    Plasmid

    #127246

    Purpose

    Asymmetric degronSwitchA_t8 fused to mCherry with KeyA-BFP

    Depositor

    Hana El-Samad

    Article

    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

    Insert

    pPGK-Key_A_e18-TagBFP-P2A-mCherry-asym_degronSwitch_A_t8-WPRE
    

    Use

    Lentiviral and Synthetic Biology

    Available Since

    June 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAN1579

    Plasmid

    #127201

    Purpose

    Progesterone inducible expression of KeyC-BFP

    Depositor

    Hana El-Samad

    Article

    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

    Insert

    pPAB1-Z3PM-tTDH1-pZ3-key_C-mTagBFP2-tENO1
    

    Use

    Synthetic Biology

    Available Since

    May 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAN1935

    Plasmid

    #127203

    Purpose

    Constitutive expression of Venus-degronSwitchA and mScarlet-degronSwitchC

    Depositor

    Hana El-Samad

    Article

    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

    Insert

    pTEF1-Venus-degronSwitch_A_t12-tENO2-pTEF2-mScarlet-Linker-degronSwitch_C-tPGK1
    

    Use

    Synthetic Biology

    Available Since

    May 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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