We narrowed to 428 results for: ipsc

1. human ETV6 gRNA-4

   Plasmid

   #133404

   Purpose

   human ETV6 gRNA-3 and 4 is a pair of gRNAs. Four ETV6 gRNA plasmids used the same backbone(Addgene plasmid#41824). ETV6 gRNA-4 target the second ETV6 exon.

   Depositor

   Linzhao Cheng

   Article

   Wu et al Stem Cell Res. 2018 May;29:6-14. doi: 10

   Insert

   ETV6 (ETV6 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti CGIP + M-AAT target

   Plasmid

   #86008

   Purpose

   lenti reporter plasmid with M-AAT-specific gRNA target-sequence, to be used with tGFP #26864

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

   Inserts

   CAG promoter
   M-AAT target
   

   Use

   Lentiviral

   Mutation

   V30M

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-GG-hUbC-EBPF2-gRNA-FAM13A

   Plasmid

   #185550

   Purpose

   Lentiviral backbone with EBFP2 reporter and gRNA targeting FAM13A

   Depositor

   Andrew Wilson

   Article

   Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

   Insert

   FAM13A gRNAs
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPV-C1-crRNA(DMD#20_DMD#23)-EF1a-BA

   Plasmid

   #204620

   Purpose

   Expression vector of crRNA (DMD#20) targeting the dystrophin intron 44 just upstream of exon 45, and crRNA(DMD#23)targeting the dystrophin intron 55 just downstream of exon 55. Blasticidin selectable.

   Depositor

   Akitsu Hotta

   Article

   Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

   Insert

   crRNA#20 (targeting DMD intron44) and crRNA#23 (targeting DMD intron 55)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-cT3G-cERP2-LHX9

   Plasmid

   #192921

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of LHX9

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   LHX9 (LHX9 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-cT3G-cERP2-HOXC9

   Plasmid

   #192910

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of HOXC9

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   HOXC9 (HOXC9 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-cT3G-cERP2-LHX1

   Plasmid

   #192905

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of LHX1

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   LHX1 (LHX1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-GG-hUbC-EBPF2-gRNA-RBMS3

   Plasmid

   #185557

   Purpose

   Lentiviral backbone with EBFP2 reporter and gRNA targeting RBMS3

   Depositor

   Andrew Wilson

   Article

   Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

   Insert

   RBMS3 gRNAs
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-GG-hUbC-EBPF2-gRNA-DSP

   Plasmid

   #185549

   Purpose

   Lentiviral backbone with EBFP2 reporter and gRNA targeting DSP

   Depositor

   Andrew Wilson

   Article

   Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

   Insert

   DSP gRNAs
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-GG-hUbC-EBPF2-gRNA-HHIP

    Plasmid

    #185551

    Purpose

    Lentiviral backbone with EBFP2 reporter and gRNA targeting HHIP

    Depositor

    Andrew Wilson

    Article

    Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

    Insert

    HHIP gRNAs
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-GG-hUbC-EBPF2-gRNA-ADGRG6

    Plasmid

    #185554

    Purpose

    Lentiviral backbone with EBFP2 reporter and gRNA targeting ADGRG6

    Depositor

    Andrew Wilson

    Article

    Werder et al Sci Adv. 2022 Jul 15;8(28):eabo6566. doi

    Insert

    ADGRG6 gRNAs
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti CGIP + Z-AAT target

    Plasmid

    #86007

    Purpose

    lenti reporter plasmid with Z-AAT-specific gRNA target-sequence, to be used with tGFP #26864

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

    Inserts

    CAG promoter
    Z-AAT target
    

    Use

    Lentiviral

    Mutation

    V30M

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti CGIP + TTR target

    Plasmid

    #86009

    Purpose

    lenti reporter plasmid with TTR_V30M-specific gRNA target-sequence, to be used with tGFP #26864

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

    Inserts

    CAG promoter
    TTR target
    

    Use

    Lentiviral

    Mutation

    V30M

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. T7-VEE-OKS-iM

    Plasmid

    #58972

    Purpose

    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Inserts

    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    cMyc (MYC Human)
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Expression

    Mammalian

    Promoter

    26S subgenomic promoter and IRES

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-cT3G-cERP2-RUNX1

    Plasmid

    #192928

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of RUNX1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    RUNX1 (RUNX1 Human)
    

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPB-cT3G-cERP2-GATA4

    Plasmid

    #192896

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of GATA4

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    GATA4 (GATA4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Codon-optimized

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHDR-FOXL2-T2A-tdTomato-PuroTK

    Plasmid

    #192892

    Purpose

    HDR donor for inserting FOXL2-T2A-tdTomato reporter

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    FOXL2-tdTomato (FOXL2 Human)
    

    Use

    CRISPR

    Mutation

    T2A-tdTomato fluorescent reporter

    Promoter

    PGK

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pgRNA-CKB

    Plasmid

    #73501

    Purpose

    gRNA expression vector (with mKate2)

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    mKate2-T2A-Bsd
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-28a(+)-FGF2-G3

    Plasmid

    #135521

    Purpose

    Engineered form of fibroblast growth factor 2 with improved thermostability

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    fibroblast growth factor 2 (FGF2 Human)
    

    Tags

    6x His tag and Agg Thrombin Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 Promoter

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAVS1-PDi-CRISPRn

    Plasmid

    #73500

    Purpose

    Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    Cas9
    rtTA
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Promoter

    CAG and TRE

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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