We narrowed to 12,329 results for: pcdna

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pcDNA3.1-Mito-AcGFP-MTn

Plasmid

#154350

Purpose

Mammalian expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Tags

AcGFP

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-human NPM1-C1

Plasmid

#160693

Purpose

Mammalian expression of FLAG-tagged NPM1 C1 deletion mutant lacking nucleic acid-binding domain (NBD).

Depositor

Charles Sherr

Article

Bertwistle et al Mol Cell Biol. 2004 Feb . 24(3):985-96.

Insert

NPM1 (NPM1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Lacks NBD domain

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HER2-DMVF-181-N

Plasmid

#87622

Purpose

N-terminal Dronpa fragment fused to the C-terminus of full-length HER2; for BiFC.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

HER2-DMVF181-N (ERBB2 Synthetic, Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-PTEN(A4)-mCherry

Plasmid

#184054

Purpose

Lysosome-targeted constitutively active mutant of phosphatase and tensin homolog (PTEN); tagged with mCherry.

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

Lyso-PTEN(A4)-mCherry (PTEN Human)

Tags

Lysosome-associated membrane protein 1 (LAMP1). a…

Expression

Mammalian

Mutation

Ser 380/385 and Thr 382/383 in PTEN mutated to Al…

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP DNAJB4

Plasmid

#19499

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB4 (DNAJB4 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA4-TO-myc-His wt-Trask

Plasmid

#31768

Depositor

Mark Moasser

Article

Spassov et al PLoS One. 2011 Apr 29;6(4):e19154.

Insert

Trask (CDCP1 Human)

Tags

His and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-mTET2 (N1041)

Plasmid

#89737

Purpose

Expresses the N1041 truncation to mouse TET2 in mammalian cells

Depositor

Yue Xiong

Article

Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

Insert

TET2 (Tet2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

N-term 1041 truncation of mouse TET2 (contains fi…

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag MFF iso2 WT

Plasmid

#74389

Purpose

Flag tagged human MFF isoform 2

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Tags

Flag

Expression

Mammalian

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3puro-mGFP-FMR1iso1-G266E

Plasmid

#225468

Purpose

Express mEGFP-fusion protein of FMR1 isoform 1 (G266E)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FMR1 isoform 1 (FMR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

G266E

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FMR1iso1-I304N

Plasmid

#225463

Purpose

Express mEGFP-fusion protein of FMR1 isoform 1 (I304N)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FMR1 isoform 1 (FMR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

I304N

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5-TEX264-deltaLRR-cSSH

Plasmid

#220210

Purpose

Mammalian expression of TEX264, 2-25 residues (LRR) truncated, C-terminal twin-Strep_HA tag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

Strep-strep-HA

Expression

Mammalian

Mutation

c.4-75del / p.2-24del

Promoter

CMV/TetO

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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p5077 pcDNA4c hBrd4 (1-594)

Plasmid

#14453

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1-594) (BRD4 Human)

Tags

His

Expression

Mammalian

Mutation

amino acids 1-594

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-miR-29b-1~29a

Plasmid

#51376

Depositor

Narry Kim

Article

Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

Insert

pri-miR-29b-1~29a (MIR29B1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP DNAJB2a

Plasmid

#19497

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB2a

Tags

GFP

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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mTiam1-mcherry-sspB in pcDNA3.1

Plasmid

#85221

Purpose

Role of Rac selective GEF domain from Tiam1 in immune cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

Inserts

mTiam1 (1030-1406) (Tiam1 Mouse)
mcherry
sspB

Tags

none

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-Rac1(G12C)

Plasmid

#224279

Purpose

Cell transfection and expression of Rac1 (G12C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rac1 (RAC1 Human)

Expression

Mammalian

Mutation

G12C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-Flag-NRBP1

Plasmid

#48197

Purpose

Expressed flag-tagged NRBP1 (nuclear receptor binding protein 1)

Depositor

Joan Conaway, Ronald Conaway

Article

Mahrour et al J Biol Chem. 2008 Mar 21;283(12):8005-13

Insert

NRBP1 (NRBP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-Nsp13-HF_GC3opt

Plasmid

#157716

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1 His-V5 Myc dMBIV

Plasmid

#176055

Purpose

Expresses Myc Mutant (delta Myc BoxIV) tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Tags

6xHis and V5

Expression

Mammalian

Mutation

deleted amino acids 304-324

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-V5 Myc dMBIIb

Plasmid

#176054

Purpose

Expresses Myc Mutant (delta Myc BoxIIb) tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Tags

6xHis and V5

Expression

Mammalian

Mutation

deleted amino acids 259-270

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-V5 Myc dMBIIa

Plasmid

#176053

Purpose

Expresses Myc Mutant (delta Myc BoxIIa) tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Tags

6xHis and V5

Expression

Mammalian

Mutation

deleted amino acids 188-199

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-V5 Myc dMBII

Plasmid

#176052

Purpose

Expresses Myc Mutant (delta Myc BoxII) tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Tags

6xHis and V5

Expression

Mammalian

Mutation

deleted amino acids 128-143

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-V5 Myc dMBI

Plasmid

#176051

Purpose

Expresses Myc Mutant (delta Myc BoxI) tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Tags

6xHis and V5

Expression

Mammalian

Mutation

deleted amino acids 44-63

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-V5 Myc dMB0

Plasmid

#176050

Purpose

Expresses Myc Mutant (delta Myc Box0) tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Tags

6xHis and V5

Expression

Mammalian

Mutation

deleted amino acids 16-33

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS DNAJA2

Plasmid

#19546

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DnaJ (Hsp40) homolog, subfamily A, member 2 (DNAJA2 Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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1571 pcDNA3 Flag HA GGA2

Plasmid

#11177

Depositor

William Sellers

Insert

GGA2 (GGA2 Human)

Tags

HA and flag

Expression

Mammalian

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only
pCDNA3-myc3-FBW5 delta F

Plasmid

#19912

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

F-box and WD repeat domain containing 5 delta F (FBXW5 Human)

Tags

myc3

Expression

Mammalian

Mutation

deleted aa1-42 (F-box)

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 hsa-mir-192

Plasmid

#26311

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-192 (MIR192 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3 βarr1 S412D Flag

Plasmid

#42196

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

β-arrestin 1 S412D (Arrb1 Rat)

Tags

Flag

Expression

Mammalian

Mutation

S412D

Promoter

CMV

Available Since

Jan. 23, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA4/HisMaxB-YAP1-S109A

Plasmid

#18981

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein S109A mutant (YAP1 Human)

Tags

His

Expression

Mammalian

Mutation

Serine 109 changed to Alanine

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only