We narrowed to 9,202 results for: sacs

1. pYZ300

   Plasmid

   #102689

   Purpose

   S. pombe gRNA entry vector with Cas9 - Sp his3 marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBEVY-T

   Plasmid

   #51232

   Purpose

   bi-directional expression vector for yeast, constitutively active, TRP1 selection

   Depositor

   Charles Miller

   Article

   Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPD/ADH1

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSMF2

   Plasmid

   #112819

   Purpose

   Expresses wild-type Rpb1 in yeast cells

   Depositor

   Stephen Fuchs

   Article

   Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

   Insert

   RPB1 (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Native promoter

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLIK 3XFLAG-EGFP-NLS hygro

   Plasmid

   #87781

   Purpose

   Inducible expression of EGFP with a C-terminal NLS sequence

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. RP156 pUAST EGFP:Gr21a

   Plasmid

   #59524

   Purpose

   N-terminal fusion of EGFP to Drosophila melanogaster Gr21a

   Depositor

   Leslie Vosshall

   Article

   Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

   Insert

   EGFP:Gr21 (Gr21a Fly)
   

   Use

   Tags

   EGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pET21a.Tat(48-57)-I-SceI

   Plasmid

   #165041

   Purpose

   Expression of Tat(48-57) conjugated I-SceI for protein purification (6XHis tag)

   Depositor

   Dokyun Na

   Article

   Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

   Insert

   I-SceI
   

   Use

   Tags

   6XHis and Tat(48-57)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. YIplac211-Sec31-yoHalo

   Plasmid

   #115423

   Purpose

   Express Sec31-yoHalo at the endogenous locus via pop-in pop-out mutagenesis

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

   Insert

   Sec31-yoHalo
   

   Use

   Tags

   yoHaloTag

   Expression

   Yeast

   Mutation

   Promoter

   Endogenous

   Available since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYZ293

   Plasmid

   #102687

   Purpose

   S. pombe gRNA entry vector with Cas9 - Sp leu1 marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Atg24 1B

   Plasmid

   #197046

   Purpose

   His6 tagged Atg24 from S. cerevisiae for expression in E. coli.

   Depositor

   Michael Ragusa

   Article

   Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

   Insert

   Atg24
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p221z-dCAS9p-t35s

    Plasmid

    #118387

    Purpose

    Entry clone containing dCAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

    Insert

    dCAS9p
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPOM033_P7_3"His5

    Plasmid

    #216458

    Purpose

    3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    3''his5
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPOM026_P2_pNMT1

    Plasmid

    #216451

    Purpose

    Regulatable promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Promoter nmt1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPOM019_P2_pRPL2102

    Plasmid

    #216444

    Purpose

    Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Promoter rpl2102
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Promoter

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPOM015_P2_pADH1

    Plasmid

    #216440

    Purpose

    Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Promoter adh1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Promoter

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPOM013_P2_pENO101

    Plasmid

    #216438

    Purpose

    Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

    Depositor

    Tomáš Pluskal

    Article

    Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

    Insert

    Promoter eno101
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Removal of any BsaI // BsmBI restriction site

    Promoter

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS416-PHO5-GFP-Osh1

    Plasmid

    #58835

    Purpose

    Expresses GFP-Tagged Osh1 in yeast

    Depositor

    Tim Levine

    Article

    Loewen et al EMBO J. 2003 May 1;22(9):2025-35.

    Insert

    Osh1 (SWH1, OSH1 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    PHO5

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pTYF-mCMV/SYN-EGFP

    Plasmid

    #19975

    Depositor

    Sergey Kasparov

    Article

    Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

    Insert

    mCMV/SYN-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21c-eIF4A

    Plasmid

    #37230

    Depositor

    Jon Lorsch

    Article

    Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

    Insert

    TIF1 (TIF1 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pFH102

    Plasmid

    #128207

    Purpose

    sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PX458 pSp Cas9 Rhino1 tagA

    Plasmid

    #211627

    Purpose

    Cas9 Rhino1 tagA

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    RHNO tagA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pIM573

    Plasmid

    #74637

    Purpose

    alias pFA6a-ymTag-BFP-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and G418 selection marker

    Depositor

    Jiří Hašek

    Article

    Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

    Insert

    ymTagBFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMAL728

    Plasmid

    #169152

    Purpose

    Encodes OptoQ-AMP6

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    A79Q

    Promoter

    Available since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTCW012

    Plasmid

    #83527

    Purpose

    Cloning destination vector for phermone mediated expression from the FUS1J2 promoter and CYC1 terminator

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pFUS1J2

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ESCRT-II

    Plasmid

    #17633

    Depositor

    James Hurley

    Article

    Hierro et al Nature. 2004 Sep 9. 431(7005):221-5.

    Insert

    pST39-Vps25(1-203) - His/Vps22(1-234) - Vps36 (1-567) (VPS36, SNF8, VPS25 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCUPI NPD-GFP

    Plasmid

    #1191

    Depositor

    Susan Lindquist

    Article

    Patino et al Science 1996 Aug 2;273(5275):622-6.

    Insert

    N-terminus of Sup35 (SUP35 Budding Yeast)
    

    Use

    Cup1 promoter

    Tags

    GFP

    Expression

    Yeast

    Mutation

    fused N-terminal Sup35 (~1-253aa) to GFP

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

26. pHES836

    Plasmid

    #89195

    Purpose

    Expresses mKate2 under control of synthetic promoter containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    pZ-mKate2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pZ

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. NES-iRFP-P4Mx1

    Plasmid

    #108144

    Purpose

    PI4P biosensor

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    X.leavis map2k1.L(32-44):iRFP:L. penomophila SidM(546-647)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LHP306 - Ub - no Lys

    Plasmid

    #32150

    Depositor

    Linda Hicke

    Article

    Terrell et al Mol Cell. 1998 Jan;1(2):193-202.

    Insert

    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    No Lys = K6R, K11R, K27R, K29R, K33A, K48R, K63R

    Promoter

    CUP1

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

29. pJAT27

    Plasmid

    #204308

    Purpose

    For generating targeting inversions. C-terminal split GFP, expressed in flight muscles.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    MHC-GFPc
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    MHC

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJAT18

    Plasmid

    #204307

    Purpose

    For generating targeting inversions. N-terminal split GFP, expressed in flight muscles.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    MHC-GFPn
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    MHC

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge