We narrowed to 8,780 results for: construct
-
Plasmid#209967PurposeContains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmidsDepositorInsertPromoter (P_sppQ)
ExpressionBacterialAvailable SinceSept. 13, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
RVD-AA5
Plasmid#173240PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorInsertNI-NI dimer RVD at Position5
UseUnspecifiedMutationnoAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
RVD-AA9
Plasmid#173244PurposeOne-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.DepositorInsertNI-NI dimer RVD at Position9
UseUnspecifiedMutationnoAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_AC-CEN_ARS_HIS
Plasmid#202129PurposeLevel 0 plasmid containing a centromere sequence that is recognized in diatoms with AC overhangs used to build level 1 constructs.DepositorInsertCEN_ARS_HIS
UseSynthetic BiologyMutationARS sequence mutated to remove sapI site for dome…Available SinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-C2-TetR-KRf-TetR
Plasmid#216270PurposeConstruct 3: TetR - Lipid binding domain KRφ - TetRDepositorInsertTetR - Lipid binding motif (KRf) - TetR
TagsEGFPExpressionMammalianAvailable SinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMET7-GAG-GW
Plasmid#194634PurposeGateway destination vector for mammalian expression of Virotrap bait constructsDepositorInsertp55 GAG
TagsPQS1ExpressionMammalianPromoterSRalphaAvailable SinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
UMG-SP-1_pET29b
Plasmid#215433PurposeExpression construct for UMG-SP-1 gene, codon optimized for expression in E. coliDepositorInsertUMG-SP-1
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
UMG-SP-2_pET29b
Plasmid#215434PurposeExpression construct for UMG-SP-2 gene, codon optimized for expression in E. coliDepositorInsertUMG-SP-2
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pconst #5-RFP
Plasmid#81079Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrPromoterConstitutive Promoter - BBa_J23110Available SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L3-Csy4-Cas9n-EGFP-L2
Plasmid#141046PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-Cas9n-2A-EGFP
ExpressionMammalianAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH-SFB-RNF169
Plasmid#99392PurposeMammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged RNF169DepositorInsertRNF169 (RNF169 Human)
TagsSFB (S protein-FLAG-Streptavidin binding peptide)ExpressionMammalianAvailable SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
NylB_pET29b
Plasmid#215414PurposeExpression construct for NylB gene, codon optimized for expression in E. coliDepositorInsertNylB
ExpressionBacterialAvailable SinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP-PKCg-C1
Plasmid#112266PurposeITRACKgamma Donor ConstructDepositorAvailable SinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pL0_EF-PtBle
Plasmid#202180PurposeLevel 0 plasmid containing a phleomycin antibiotic cassette (fcpF promoter) with EF overhangs used to build a level 1 construct.DepositorInsertShBle expression cassette
UseSynthetic BiologyMutationNoneAvailable SinceAug. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYB-Dual(mNG(yeast-opt))
Plasmid#216727PurposeDual-species protein expression vector for high-level and inducible protein expression of mNeonGreen (codon optimized for yeast) in both yeast and bacteria from the same construct.DepositorInsertmNeonGreen
ExpressionBacterial and YeastPromoterpGAL1(yeast) and pT7-RNAP(bacteria)Available SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-mCherry-NS-MCS
Plasmid#75179PurposemCherry (red) fluorophore with multiple cloning site for use with inducible N-terminal fusions.DepositorInsertmCherry-NS-MCS
UseThree prime entry vector for multisite gateway th…Promotern/aAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS:Chronos-tdTomato
Plasmid#124232PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin Chronos fused to the red fluorescent protein tdTomatoDepositorInsertChronos-tdTomato
UseZebrafish expressionTagstdTomatoPromoter14xUAS-E1bAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mGrn-myc
Plasmid#178944PurposeConstruct for expressing murine progranulin (Grn) in AAVDepositorAvailable SinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tt-NylC_pET28a
Plasmid#215431PurposeExpression construct for Tt-NylC gene, codon optimized for expression in E. coliDepositorInsertTt-NylC
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pconst #1-RFP
Plasmid#81075Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrPromoterConstitutive Promoter - BBa_J23119Available SinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-mKOFP2-CAAX
Plasmid#75155PurposeMembrane targeted KOFP2 (orange) fluorophore for use with 5’ promoters.DepositorInsertmKOFP2-CAAX
UseMiddle entry vector for multisite gateway three f…Promotern/aAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKS913.CB18.31
Plasmid#69569PurposeTCR beta chain genomic transgenic construct for creating OT-1 miceDepositorAvailable SinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pconst #4-RFP
Plasmid#81078Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrPromoterConstitutive Promoter - BBa_J23107Available SinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-deltaA1-VWF
Plasmid#124823PurposeExpresses multimeric human VWF protein without A1 domain. This construct has no tag.DepositorInsertVWF without A1 domain (VWF Human)
ExpressionMammalianMutationDeleted A1 domainPromoterCMVAvailable SinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPA-RL-FL
Plasmid#78863PurposeDULIP Tandem Construct used for data normalizationDepositorInsertFirefly
UseExpression vectorTagsProtein A, Renilla luciferaseExpressionMammalianPromoterCMVAvailable SinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSSA-PMG (pXK-1649)
Plasmid#208857PurposeSSA-base selectable traffic light reporter (TLR) for helping to screen gene-editing positive cells. Key Construct: CMV-PuroR.L-mCherry-PuroR.R-GFP.DepositorTypeEmpty backboneExpressionMammalianPromoterCMVAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_EF-H4
Plasmid#202178PurposeLevel 0 plasmid containing a terminator sequence from gene 34971 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.DepositorInsertH4 terminator
UseSynthetic BiologyMutationNoneAvailable SinceAug. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_EF-fcpA
Plasmid#202164PurposeLevel 0 plasmid containing a terminator sequence from gene 18049 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.DepositorInsertfcpA terminator
UseSynthetic BiologyMutationNoneAvailable SinceAug. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-H4
Plasmid#202122PurposeLevel 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertH4 promoter
UseSynthetic BiologyMutationNoneAvailable SinceAug. 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_AC-P49
Plasmid#202120PurposeLevel 0 plasmid containing the promoter from gene 49202 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.DepositorInsertP49 promoter
UseSynthetic BiologyMutationNoneAvailable SinceAug. 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
p3E-EGFP-NS-MCS
Plasmid#75180PurposeEGFP (green) fluorophore with multiple cloning site for use with inducible N-terminal fusions.DepositorInsertEGFP-NS-MCS
UseThree prime entry vector for multisite gateway th…Promotern/aAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
1352wUV2 F3bbs kan NNNTGGGCGk
Plasmid#18756DepositorInsert3 tandem zinc fingers
ExpressionBacterialMutationA Kan cassette (1090bp) is inserted between two b…Available SinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
1352wUV2 F1bbs kan GCGTGGNNN
Plasmid#18753DepositorInsert3 tandem zinc fingers
ExpressionBacterialMutationA Kan cassette (1090bp) is inserted between two b…Available SinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
ninaE-dsocs
Plasmid#26857DepositorInsertSlow Termination of Phototransduction (stops Fly)
TagsmycExpressionInsectMutationThis STOPS construct contains a mutated/truncated…Available SinceApril 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pU6_T7_INT[6xminimal_Puf1]
Plasmid#107608PurposeTransient expression of an "INT" construct [bearing six minimal Puf1 stem-loops; 'INT' design: Shechner et al., Nature Methods 2015], in mammalian cells, U6 promoterDepositorInsert6x minimal Puf1 motif
UseSynthetic BiologyExpressionMammalianPromoterU6Available SinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIhh742bp-Luc
Plasmid#53605PurposeReporter construct containing a 742 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterAvailable SinceJune 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS:CheRiff-tdTomato
Plasmid#124234PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CheRiff fused to the red fluorescent protein tdTomatoDepositorInsertCheRiff-tdTomato
UseZebrafish expressionTagstdTomatoPromoter14xUAS-E1bAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIBA2-SpyTag-MBP
Plasmid#107422PurposeControl construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.DepositorInsertMaltose-binding protein (MalE)
TagsOmpA signal peptide, SpyTag, and StrepIIExpressionBacterialPromoterTetAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK-EdnrbE2-Sox10-FLAG-tev-AVI
Plasmid#127775PurposeModified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Sox10 in frame with a Flag-tag and Avi-tagDepositorAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-EAF pBacPAK8
Plasmid#15323DepositorInsertELL Associated Factor (EAF1 Human)
UseBaculovirus constructionTags6x His and FLAGPromoterpolyhedrinAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only