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We narrowed to 12,214 results for: LEA

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Showing: 3181 - 3200 of 12214 results

  1. pCAGGS-SARS2-S-FKO (C-flag)

    Plasmid
    #159364
    Purpose
    Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at C-terminus.
    Depositor
    Hyeryun Choe, Michael Farzan
    Publication
    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S protein (S SARS-CoV-2)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Furin-cleavage site mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    159364-plasmid-map-sequence-id-310911

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  2. pBJG1 T7-8xHis-OGT K842M

    Plasmid
    #154272
    Purpose
    Bacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Publication
    Lazarus et al Nature. 2011 Jan 27;469(7331):564-7. doi
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    Lysine 842 changed to Methionine- bacterial codon…
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154272-plasmid-map-sequence-id-304833

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  6. Membrane mCherry

    Plasmid
    #210470
    Purpose
    Plasma membrane localizing mCherry using Human LCK proto-oncogene sequence
    Depositor
    Amro Hamdoun
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LCK membrane mCherry (LCK Human, A.victoria, Synthetic)
    Use
    Synthetic Biology; Sea urchin, mammal, zebrafish
    Tags
    mCherry
    Available since
    Dec. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    210470-plasmid-map-sequence-id-416828

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  8. pcDNA3-Venus-StagRFP-AktAR

    Plasmid
    #173020
    Purpose
    Yellow-red FRET-based Akt/PKB activity reporter for multiplexed imaging applications.
    Depositor
    Jin Zhang
    Publication
    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Venus-StagRFP-AktAR
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    173020-plasmid-map-sequence-id-345236

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  9. pAAV-CMV-CASANOVA

    Plasmid
    #113036
    Purpose
    AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
    Depositor
    Dominik Niopek
    Publication
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    Use
    AAV, CRISPR, and Synthetic Biology
    Expression
    Mammalian
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    113036-plasmid-map-sequence-id-226953

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  11. pRVdG-EGFP-N-P-M-nucBFP-SynPhRFP-L

    Plasmid
    #52478
    Purpose
    Expresses EGFP, nuclear mTagBFP, and Synaptophysin-TagRFP-T
    Depositor
    Sebastian Seung
    Publication
    Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    EGFP
    nuclear mTagBFP
    Synaptophysin-TagRFP-T
    Use
    Rabies virus
    Tags
    TagRFP-T
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    52478-plasmid-map-sequence-id-81361

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  12. p663-UBC-miniTurbo-V5-CDKL5_IDG-K

    Plasmid
    #170578
    Purpose
    Gateway destination clone of CDKL5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CDKL5 (CDKL5 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    E854G- please see depositor comments
    Promoter
    Ubiquitin
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    170578-plasmid-map-sequence-id-340993

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  14. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104911
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    104911-plasmid-map-sequence-id-269032

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  16. pCS2+ cMyc Dam_f_GFPoNLS

    Plasmid
    #85820
    Purpose
    iDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Publication
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    cMyc Dam_f_GFPoNLS
    Tags
    cMyc and oNLS (optimized nuclear localization sig…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A.
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85820-plasmid-map-sequence-id-170474

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  17. pEGFP-ninein C-ter

    Plasmid
    #73523
    Purpose
    Encodes the C-terminus of mouse ninein (residues 1874-2113) with an EGFP tag at its N-terminus
    Depositor
    Michel Bornens
    Publication
    Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ninein C-ter (residues 1874-2113) (Nin Mouse)
    Tags
    EGFP tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  19. pLV-REPORT(EF1a)

    Plasmid
    #172326
    Purpose
    LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    David Bersten
    Publication
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    monomeric nuclear Tomato
    d2eGFP
    HygR
    Use
    Lentiviral and Synthetic Biology
    Tags
    3x SV40 NLS
    Expression
    Mammalian
    Promoter
    EF1-alpha
    Available since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    172326-plasmid-map-sequence-id-354701

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Showing: 3181 - 3200 of 12214 results