4,082 results

1. pcDNA3-EGFP-Cdc42-T17N

   Plasmid

   #12976

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   Cdc42 DN (CDC42 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   T17N Dominant negative

   Promoter

   CMV

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIMAY

   Plasmid

   #68939

   Purpose

   E. coli/staphylococcal temperature-sensitive plasmid for allelic exchange

   Depositor

   Tim Foster

   Article

   Monk et al MBio. 2012 Mar 20;3(2). pii: e00277-11.

   Type

   Empty backbone

   Use

   Staphylococcal allelic exchange vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-DIO ChETA-EYFP

   Plasmid

   #26968

   Purpose

   AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   hChR2(E123T/H134R)-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T, H134R

   Promoter

   EF1alpha

   Available since

   Jan. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW57-GFP-2A-MCS

   Plasmid

   #71783

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXAT2

   Plasmid

   #80494

   Purpose

   AAVS1 sgRNA expression vector

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   AAVS1 sgRNA-T2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBMN YFP-DHFR(DD)

   Plasmid

   #29326

   Depositor

   Thomas Wandless

   Article

   Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

   Insert

   YFP-DHFR(DD)
   

   Use

   Retroviral

   Tags

   HA and YFP

   Expression

   Mutation

   Change Arg 12 to His (R12H),Asn 18 Thr (N18T), Va…

   Promoter

   Available since

   April 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-flag YAP2 5SA

   Plasmid

   #27371

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

   Insert

   YAP2 (YAP1 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Serines at positions 61, 109, 127, 128, 131, 163,…

   Promoter

   Available since

   Feb. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. ERmoxGFP

   Plasmid

   #68072

   Purpose

   mammalian expression of ER localized moxGFP

   Depositor

   Erik Snapp

   Article

   Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

   Insert

   moxGFP
   

   Use

   Tags

   KDEL ER retention sequence and bovine prolactin s…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. multi-shRNA vector

   Plasmid

   #12391

   Depositor

   Zuoshang Xu

   Article

   Xia et al Biotechniques. 2006 Jul . 41(1):64-8.

   Insert

   mSOD2 shRNA (Sod2 Mouse)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. Flag-HA-OTUD5

    Plasmid

    #22610

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    OTUD5 (OTUD5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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