We narrowed to 78,728 results for: TRI

1. pENTR221 UBR5 ∆HECT

   Plasmid

   #81065

   Purpose

   Gateway entry vector encoding full length UBR5 C2768A (∆HECT)

   Depositor

   Darren Saunders

   Article

   Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

   Insert

   Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆HECT (UBR5 Human)
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   C2768A (bp t8302g and g8303c)

   Promoter

   N/A

   Available since

   Jan. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE32-pr-mEosFP (mEosFP-V69T)

   Plasmid

   #99213

   Purpose

   Bacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-mEosFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   monomeric variant with mutation V69T for primed c…

   Promoter

   T5

   Available since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. hEGFR-mEGFP

   Plasmid

   #182878

   Purpose

   Mammalian expression of epidermal growth factor receptor (EGFR) fused to mEGFP

   Depositor

   Salvatore Chiantia

   Article

   Tzoneva et al Biomolecules. 2020 May 22;10(5). pii: bi

   Insert

   hEGFR-mEGFP (EGFR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS2303-Tier1-CoTC-PPGK-NLuc_TB

   Plasmid

   #169548

   Purpose

   Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   CoTC-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Twitch-2B pcDNA3

   Plasmid

   #49531

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

   Insert

   Twitch-2B
   

   Use

   Tags

   ECFP and cpCit174

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. TPP1-bio-His

   Plasmid

   #52090

   Purpose

   Expresses full-length Tripeptidyl-peptidase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   TPP1 (TPP1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. CB6-GFP-TAOK2

   Plasmid

   #197110

   Purpose

   Expression of GFP-tagged TAOK2 / PSK1 in mammalian cells

   Depositor

   Jonathan Morris

   Article

   Garg et al J Cell Sci. 2020 Mar 16;133(6):jcs235895

   Insert

   TAOK2 (TAOK2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Wild Type

   Promoter

   CMV

   Available since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody

   Plasmid

   #138130

   Purpose

   Expresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   mCherry-anti-mouse-IgG(kappa)-nanobody
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ef1a-DIO_EGFP-GFE3

   Plasmid

   #79871

   Purpose

   For generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-GFE3
   

   Use

   AAV, Cre/Lox, and Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV hSyn FLEx-FRT hM3Dq-mCherry

    Plasmid

    #161575

    Purpose

    Expression of hM3Dq-mCherry in a Flp-dependent fashion

    Depositor

    Robert Malenka, Kevin Beier

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    hM3Dq-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    hSyn

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS416 Gal Q331K YFP

    Plasmid

    #27450

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Glutamine 331 mutated to Lysine (Q331K)

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pCMV3Tag8li-hDDX41-HA

    Plasmid

    #165469

    Purpose

    Human DDX41 mammalian expression vector with C-terminal HA tag.

    Depositor

    Martin Dorf

    Article

    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

    Insert

    DEAD-box helicase 41 (DDX41 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOPINK-OTUD2 (full-length, aa 1-348)

    Plasmid

    #61409

    Purpose

    Expresses human OTUD2 (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD2 (YOD1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOPINK-OTUD1 (full-length, aa 1-481)

    Plasmid

    #61405

    Purpose

    Expresses human OTUD1 (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD1 (OTUD1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-DIO-H2B.APEX-P2A-EGFP

    Plasmid

    #182825

    Purpose

    Cre-dependent expression of H2B-APEX fusion

    Depositor

    Yevgenia Kozorovitskiy

    Article

    Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

    Insert

    H2B.APEX-P2A-EGFP
    

    Use

    AAV

    Tags

    EGFP reporter (P2A)

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. U6-sgRNA-GFP

    Plasmid

    #200068

    Purpose

    For the knock down of GFP with an astrocyte specific mCherry expression

    Depositor

    Baljit Khakh

    Article

    Ollivier et al Nature. 2024 Mar;627(8003):358-366. doi:

    Insert

    U6-sgRNA-GFP
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    GfaABC1D-mCherry

    Expression

    Mutation

    Promoter

    U6, GfaABC1D

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Dominant negative Hsp90 beta

    Plasmid

    #22480

    Depositor

    William Sessa

    Article

    Miao et al Arterioscler Thromb Vasc Biol. 2008 Jan

    Insert

    Dominant negative Hsp90 (HSP90AB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. tetO-FUW-eGFP-RHOA-Q63L

    Plasmid

    #73081

    Purpose

    Lentiviral transfer vector for tet-inducible expression of EGFP tagged human RHOA-Q63L

    Depositor

    Andrew Putnam

    Article

    Kong et al Sci Rep. 2013 Dec 11;3:3474. doi: 10.103

    Insert

    RHOA-Q63L (RHOA Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Q63L Constitutively active

    Promoter

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAV5B+longBRD4

    Plasmid

    #157792

    Purpose

    Expression of long isoform of BRD4

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    long BRD4 (BRD4 Human)
    

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    Promoter

    polyhedron

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. LVDP-CArG-RE-GPR

    Plasmid

    #89762

    Purpose

    Quantitative measurement of CArG response element activity.

    Depositor

    Stelios Andreadis

    Article

    Mistriotis et al Stem Cells. 2017 Jan;35(1):207-221. doi:

    Insert

    6X CArG-RE
    

    Use

    Lentiviral

    Tags

    CMV-ZsGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCEP4-myc-HLA-A3

    Plasmid

    #135505

    Purpose

    Mammalian expression of myc-tagged HLA-A*03:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*03:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-hGfap-R-CaMP1.07-WPRE-SV40

    Plasmid

    #164140

    Purpose

    AAV vector to express the red Ca2+ indicator R-CaMP1.07 in astrocytes.

    Depositor

    Hajime Hirase, Hirokazu Hirai

    Article

    Oe et al Nat Commun. 2020 Jan 24;11(1):471. doi:

    Insert

    R-CaMP1.07
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hGFAP

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pST_119_LVL2 cam

    Plasmid

    #179333

    Purpose

    NT-CRISPR plasmid for integration of multiple gRNAs.

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, sfGFP dropout to be replaced with gRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGPTVII-SED1

    Plasmid

    #232615

    Purpose

    SED1 biosensor for expression in Arabidopsis thaliana

    Depositor

    Jose Dinneny

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 Biosensor
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pUBQ10

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pOPINK-OTUD6B (full-length, aa 1-293)

    Plasmid

    #61418

    Purpose

    Expresses human OTUD6B (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD6B (OTUD6B Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pOPINK-Cezanne (OTU, aa 53-446)

    Plasmid

    #61581

    Purpose

    Expresses human Cezanne (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    Cezanne (OTUD7B Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-52 and aa 447-843.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCold TDP43 Q331K

    Plasmid

    #27454

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Factor Xa and His6

    Expression

    Bacterial

    Mutation

    Glutamine 331 mutated to Lysine (Q331K)

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

28. pOPINK-OTUD6A (full-length, aa 1-288)

    Plasmid

    #61416

    Purpose

    Expresses human OTUD6A (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD6A (OTUD6A Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CDK1as_T2A_puromycin

    Plasmid

    #118595

    Purpose

    One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.

    Depositor

    William Earnshaw

    Article

    Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

    Insert

    CDK1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFLAG-Tetherin

    Plasmid

    #41070

    Depositor

    Paul Spearman

    Article

    Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

    Insert

    Tetherin (BST2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only