We narrowed to 13,473 results for: Mpl
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Plasmid#136794PurposeMammalian expression of the centrosomal protein Cep41 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep41 (CEP41 Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-sTagRFP-TUBA1B
Plasmid#207768PurposeDonor template for Blast-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorInsertTUBA1B Homology Arms flanking a Blast-sTagRFP Cassette (TUBA1B Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEBTet-SNAP-α-tubulin
Plasmid#136791PurposeMammalian expression of the centrosomal protein _-tubulin N-terminally fused to SNAP-tagDepositorInsertSNAP-TUBA (DNMBP Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-MRPP3-His
Plasmid#67866Purposebacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tagDepositorInsertMRPP3 (KIAA0391 Human)
TagsHisExpressionBacterialMutationAmino acid 46 of recombinant MRPP3 is preceded by…PromoterT7Available SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6
Plasmid#192788PurposeaGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labelingDepositorInsertantiGFP nanobody enhancer
TagsH6 and ybbRExpressionBacterialAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
HOXB3 (human) FLAG pSP65
Plasmid#8523DepositorInsertHOXB3 (HOXB3 Human)
UseIn vitro transcription/translationTagsFLAGExpressionBacterialMutationContains 5 additional amino acids before ATG from…Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AviMta3-3xFiBsd
Plasmid#140962Purposefull-length wt Avi-Mta3-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cellsDepositorInsertmetastasis associated 3 (Mta3 Mouse, Synthetic)
Tags3xFLAG, Avi, and TEVExpressionMammalianPromoterCAGAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot9_18-285_S
Plasmid#147480PurposeMammalian Expression of HsNot9_18-285DepositorInsertHsNot9_18-285 (CNOT9 Human)
ExpressionMammalianAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot9_18-285_S
Plasmid#147482PurposeMammalian Expression of HsNot9_18-285DepositorInsertHsNot9_18-285 (CNOT9 Human)
ExpressionMammalianAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
CD8a-hnRNP-M-EGFP
Plasmid#86054PurposeA mammalian expression plasmid encoding CD8a luminal and transmembrane domains followed by hnRNP-M (PY nuclear localization signal of hnRNP-M) and C-terminus EGFP.DepositorInsertCD8a (CD8A Human)
TagsGFPExpressionMammalianMutationCD8a luminal and transmembrane domians along with…PromoterCMVAvailable SinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-IRESMuro
Plasmid#82336PurposeBase vector targeting genes to the GAPDH locus of human cells. Inserted genes are expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInsertIRESMuro
UseSynthetic BiologyExpressionMammalianAvailable SinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-XL4 ASXL1 (p.R404X) 3x FLAG
Plasmid#74263PurposeCancer-associated ASXL1 truncation mutant in mammalian expression vectorDepositorInsertASXL1 (ASXL1 Human)
Tags3X FLAGExpressionMammalianMutationp.R404X truncationPromoterCMVAvailable SinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-INTS3-C
Plasmid#128417PurposeExpress INTS3 C-termini (514-1042 aa) in E. coli with a GST tag (N-terminal)DepositorInsertINTS3 (INTS3 Human)
TagsGSTExpressionBacterialMutationINTS3 C-terminal region (514-1042 aa)PromoterTac promoterAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST27-GST-ATXN1 FL(92Q)-S776D
Plasmid#21757DepositorInsertAtaxin-1 (ATXN1 Human)
TagsGSTExpressionMammalianMutationinsert contains a stretch of 92 Q(Glutamines) and…PromoterCMVAvailable SinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEBTetDBl-CLIP-α-tubulin
Plasmid#136841PurposeMammalian expression of the centrosomal protein _-tubulin N-terminally fused to CLIP-tagDepositorInsertCLIP-TUBA (DNMBP Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-Cep70
Plasmid#136821PurposeMammalian expression of the centrosomal protein Cep70 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep70 (CEP70 Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MBP-Foldon-ATG9 (830-839)
Plasmid#188091PurposeTo make ATG9 C-terminal tail trimer for expression in mammalian cellsDepositorAvailable SinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-VSTM2L-COMP5AP-AviTag-9xHis
Plasmid#157146PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-AuroraB L154G/H250Y
Plasmid#108492Purposeexpression of EGFP-AuroraB Analog sensitive (L154G/H250Y)DepositorInsertAuroraB (AURKB Human)
TagsEGFPExpressionMammalianMutationAnalog sensitive L154G/H250YPromoterCMVAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FRT-TO-FH-Nsp7-Nsp8_GC3opt
Plasmid#157692Purposemammalian expression of N-terminally Flag-His8 tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoterDepositorInsertFH-Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
TagsFlag-His8ExpressionMammalianMutationcodon optimized; gene expression was optimized by…Available SinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEBTetBl-CLIP-Cep78
Plasmid#136876PurposeMammalian expression of the centrosomal protein Cep78 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep78 (CEP78 Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-ADCY6-C2 (917-1165 aa)
Plasmid#113904PurposeHis-S tagged cytoplasmic catalytic domain 2 of Adenylate Cyclase 6DepositorInsertAdenylate Cyclase 6 cytoplasmic catalytic domain 2 (ADCY6 Human)
TagsHis-SExpressionBacterial, Insect, and Mamm…MutationPlease see Depositor CommentsPromoterT7, CMVAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-ADCY6-C1(306-672 aa)
Plasmid#113903PurposeHis-S tagged cytoplasmic catalytic domain 1 of Adenylate Cyclase 6DepositorInsertAdenylate Cyclase 6 cytoplasmic catalytic domain 1 (ADCY6 Human)
TagsHis-SExpressionBacterial, Insect, and Mamm…PromoterT7, CMVAvailable SinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3-Flag-AuroraB KD (K106R)
Plasmid#108488Purposeexpression of Flag-AuroraB kinase death (K106R)DepositorInsertAuroraB (AURKB Human)
TagsFlagExpressionMammalianMutationKinase death K106APromoterCMVAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_190-303
Plasmid#118822PurposeBacterial expression of hnRNPA2 truncation (residues 190 to 302).DepositorInserthnRNPA2 (HNRNPA2B1 Human)
TagsHis tag and TEVExpressionBacterialMutationcontains amino acids 190-302Available SinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SEMA3G-COMP5AP-AviTag-9xHis
Plasmid#157539PurposeMammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-3xNLS-OptoJNKi
Plasmid#89740PurposeExpression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, wild-type photosensor, inhibitor JIP11DepositorInsertNLS-OptoJNKi
UseFluorescent proteinTagsmCherryExpressionMammalianPromoterCMVAvailable SinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N3/hArf5(WT)-EGFP
Plasmid#79534PurposeExpresses C-terminally EGFP-tagged Arf5(WT) in mammalian cellsDepositorAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAC8_PH-6His-Nter-GWs-Lox (VE5587)
Plasmid#163768PurposeTransfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter 6 His tag under the pH promoter.DepositorInsertN-terminal 6His tag
Tags6 His TagExpressionInsectPromoterPHAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-PH-10His-Cter (VE5631)
Plasmid#161802PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter10His tag under the p10 promoter.DepositorInsertC-terminal 10 His tag
Tags10 HisExpressionInsectPromoterp10Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-P10-mCherry-pH-3C-TwinStrep (VE5621)
Plasmid#139770PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter TwinStrep tag under the PH promoter.DepositorInsertC-terminal TwinStrep tag
Tags3C - TwinStrep tagPromoterPHAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-p10-10His-Cter (VE5742)
Plasmid#139776PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter10His tag under the pH promoter.DepositorInsertC-terminal 10His tag
Tags10 HisExpressionInsectPromoterPHAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-p10-Flag-Cter(VE5738)
Plasmid#161798PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter Flag tag under the p10 promoter.DepositorInsertC-terminal Flag tag
TagsFlagExpressionInsectPromoterp10Available SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_PH-10His-Nter-GWs-Lox (VE5588)
Plasmid#161806PurposeTransfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter 10 His tag under the pH promoter.DepositorInsertN-terminal 10His tag
Tags10 HisExpressionInsectPromoterPHAvailable SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-p10-mCh PH-mCh (VE5625)
Plasmid#139769PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an mCherry cDNA under each promoter.DepositorInsertmCherry fluorescent protein
ExpressionInsectPromoterPH or p10Available SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-707pUC19-tNGFR-P2A-TTC37
Plasmid#186122PurposeKnockin of truncated NGFR to target geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBrain-GFP-TACC3KDP(S558A)-shTACC3
Plasmid#59357PurposeExpresses GFP-tagged human TACC3 (RNAi-resistant) with S558A substitution and knocks down endogenous TACC3 via shRNA.DepositorInsertTransforming Acidic Coiled Coil protein 3 (TACC3 Human)
UseRNAiTagsGFPExpressionMammalianMutationSilent mutations in amino acids 30-33 to confer s…PromoterCMVAvailable SinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MBP-Foldon-ATG9 (692-839)
Plasmid#188087PurposeTo make ATG9 C-terminal tail trimer for expression in mammalian cellsDepositorAvailable SinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-mtagtdTom_IRESMuro
Plasmid#82355PurposeVector targeting the mTagtandem tomato gene to the GAPDH locus of human cells. It is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInsertmtagTandemTomato-IRESMuro
UseSynthetic BiologyExpressionMammalianAvailable SinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(g8)
Plasmid#89727PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing 33-nt spacer (referred to as 'wt') matching a protospacer in the M13 phage genome.DepositorInsertCRISPR array
ExpressionBacterialAvailable SinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKCAG-mCherry-OptoJNKi
Plasmid#89738PurposeExpression of light-regulated JNK inhibitor, wild-type photosensor, inhibitor JIP11DepositorInsertOptoJNKi
UseFluorescent proteinTagsmCherryExpressionMammalianPromoterCAGAvailable SinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-Cep27
Plasmid#136808PurposeMammalian expression of the centrosomal protein Cep27 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep27 (HAUS2 Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMP108 Nanog-Venus-2a-mCherry
Plasmid#159743PurposeNanog targeting vector to insert fluorescent reporter of protein and gene expression levels from endogenous locus in mouse embryonic stem cells. Please see depositor comments for more detail.DepositorAvailable SinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-NES-OptoJNKi
Plasmid#89739PurposeExpression of light-regulated JNK inhibitor, mCherry-tagged, localised to cytoplasm, wild-type photosensor, inhibitor JIP11DepositorInsertNLS-OptoJNKi
UseFluorescent proteinTagsmCherryExpressionMammalianPromoterCMVAvailable SinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKCAG-H2BmCherry-OptoJNKi
Plasmid#89741PurposeExpression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11DepositorInsertOptoJNKi
UseOptogeneticsTagsHistone2B and mCherryExpressionMammalianPromoterCAGAvailable SinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H96
Plasmid#170342PurposeCFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertCFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
ExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST27-GST-ATXN1 FL(30Q)-S776D
Plasmid#21755DepositorInsertAtaxin-1 (ATXN1 Human)
TagsGSTExpressionMammalianMutationinsert contains a stretch of 32 amino acids (30 Q…PromoterCMVAvailable SinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PTBP1-RRM12CCtoSS
Plasmid#89155PurposeRecombinant expression of PTBP1-RRM12 C250S,C251S in E.coliDepositorInsertPolypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)
Tagshexa HisExpressionBacterialMutationmutations: Cys 250 to Ser, Cys 251 to SerPromoterT7Available SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pADSL-2XStrep
Plasmid#125684Purposeexpresses human ADSL protein with a C-terminal 2XStrep affinity tagDepositorAvailable SinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIRES:mTREK-1(K271Q)
Plasmid#133273PurposeMammalian expression vector. It will generate the mouse TREK-1 channel full lenght K271Q mutant fused to a N-terminal HA tagDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
TagsHAExpressionMammalian and YeastMutationK271QAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only