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Showing: 8001 - 8050 of 9723 results

  3. BmR1_04g07470-bio-His

    Plasmid
    #108037
    Purpose
    Expresses enzymatically monobiotinylated full-length BmR1_04g07470 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.
    Insert
    BmR1_04g07470
    Tags
    ratCD4d3+4,biotinylation sequence, 6xHis
    Expression
    Mammalian
    Mutation
    all potential N-linked glycosylation sites (N-X-S…
    Promoter
    CMV
    Available Since
    Oct. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    108037-plasmid-map-sequence-id-213138

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  6. pMinDis.X513-iNicSnFR3a-(CC93)-PM

    Plasmid
    #125122
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine
    Depositor
    Henry Lester
    Article
    Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018
    Insert
    iNicSnFR3a-(CC93)-PM
    Tags
    Ig-K-Chain and membrane anchoring sequence-PDGFR
    Expression
    Mammalian
    Mutation
    NA
    Promoter
    T7
    Available Since
    Oct. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    125122-plasmid-map-sequence-id-245433

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  9. LUC01 (L/Sec/P)

    Plasmid
    #112144
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.
    Insert
    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
    Expression
    Mammalian
    Mutation
    Cysteine 258 mutated to selenocysteine (U)
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112144-plasmid-map-sequence-id-214867

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  11. SBP-GFP-Tac-TC

    Plasmid
    #162506
    Purpose
    Expresses GFP tagged partial length Tac (TMD and cytosolic tail) in RUSH vector in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    GFP and SBP tagged partial length Tac (TMD and cytosolic tail) (IL2RA Human)
    Tags
    GFP and SBP
    Expression
    Mammalian
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162506-plasmid-map-sequence-id-323518

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  13. pGW-Dronpa-Cav1.2

    Plasmid
    #182333
    Purpose
    L-type voltage-gated calcium channel α1C subunit tagged with Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    Cav1.2-Dronpa (CACNA1C Rabbit)
    Tags
    Full-length rabbit Cav1.2.
    Expression
    Mammalian
    Mutation
    T1066Y, Q1070M
    Promoter
    CMV
    Available Since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182333-plasmid-map-sequence-id-363204

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  19. pET22b Ag43 160N 6H sfGFP-R5 AmpR

    Plasmid
    #225139
    Purpose
    Recombinant Ag43 protein fused to silaffin-R5 and sfGFP. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.
    Depositor
    Urartu Seker
    Article
    Kirpat Konak et al Mater Today Bio. 2022 Oct 10;17:100461.
    Insert
    Ag43 protein fused to silaffin-R5 and sfGFP
    Tags
    His tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    225139-plasmid-map-sequence-id-460505

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  21. pQ-NFY-A-GB-HC

    Plasmid
    #171081
    Purpose
    Co-expresses of NFY complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding the three subunit NF-Y, with untagged NFYA, GST-NFYB, and 6-His-NFYC
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    NF-YA (NFYA Human)
    NF-YB (NFYB Human)
    NF-YC (NFYC Human)
    Tags
    GST and His
    Expression
    Bacterial
    Promoter
    T5 promoter
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171081-plasmid-map-sequence-id-335631

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  22. LUC05 (L/Cys/P)

    Plasmid
    #112146
    Purpose
    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity
    Depositor
    Paul Copeland
    Article
    Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.
    Insert
    Luciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
    Expression
    Mammalian
    Mutation
    none
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112146-plasmid-map-sequence-id-214888

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  36. p2R3a-3AT

    Plasmid
    #71273
    Purpose
    Entry clone containing 3AT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Ari Pekka Mähönen
    Article
    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246
    Insert
    T3A ribulose-1,5-bisphosphate carboxylase 3A subunit terminator
    Use
    Gateway
    Available Since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    71273-plasmid-map-sequence-id-129830

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  42. pD/Paro-IFN

    Plasmid
    #205439
    Purpose
    A donor vector plasmid for Cre-mediated integration of paromomycin resistance (Paro) and dog IFNα-4 (IFNα-4) genes
    Depositor
    Masamichi Kamihira
    Article
    Huang et al J Biosci Bioeng. 2021 Nov;132(5):469-478
    Inserts
    dog interferon α-4 gene
    paromomycin resistance gene
    Use
    Cre/Lox; Chlamydomonas expression
    Promoter
    Heat shock protein 70A (HSP70A)/ribulose bisphosp…
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    205439-plasmid-map-sequence-id-408672

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  46. pNS19-LV-mutRFP-2A-GFP

    Plasmid
    #113716
    Purpose
    Lentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)
    Depositor
    Gerald Schwank
    Article
    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1
    Insert
    mutRFP-2A-GFP-IRES-Puro
    Use
    Lentiviral; Reporter system
    Expression
    Mammalian
    Mutation
    Y64L (mutRFP)
    Available Since
    Aug. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    113716-plasmid-map-sequence-id-220030

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Showing: 8001 - 8050 of 9723 results