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We narrowed to 19,527 results for: ACE;

Showing: 6441 - 6480 of 19527 results

  4. pMT2 Cav2.2 DomI and DomII

    Plasmid
    #89891
    Purpose
    Mammalian expression of Cav2.2 DomI and DomII, this contains the first 2 domains (amino acids 1 -1154) of Cav2.2
    Depositor
    Annette Dolphin
    Article
    Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.
    Insert
    Cacna1b (CACNA1B )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Stop codon after amino acid 1154 (this is a trunc…
    Promoter
    adenovirus major late promoter
    Available since
    April 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    89891-plasmid-map-sequence-id-176913

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  5. His8:MBP-tev-Ala-eK-Flv

    Plasmid
    #83393
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Article
    Mot et al New Phytol. 2017 Mar 9. doi: 10.1111/nph
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83393-plasmid-map-sequence-id-174595

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  6. His8:MBP-tev-Ile-eK-Flv

    Plasmid
    #83392
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Article
    Mot et al New Phytol. 2017 Mar 9. doi: 10.1111/nph
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83392-plasmid-map-sequence-id-174594

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  7. His8:MBP-tev-Tyr-eK-Flv

    Plasmid
    #83391
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Article
    Mot et al New Phytol. 2017 Mar 9. doi: 10.1111/nph
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83391-plasmid-map-sequence-id-174593

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  9. pVH003

    Plasmid
    #80397
    Purpose
    Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-YFP-AAV. This plasmid was used for microscopy tracking experiments
    Depositor
    Richard Murray
    Article
    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.
    Inserts
    Anti-scaffold - Venus YFP
    CusR (cusR )
    AraC (araC )
    Use
    Synthetic Biology
    Tags
    Fused by GS linker to LZx domain and Venus has N-…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    80397-plasmid-map-sequence-id-155890

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  10. pET22-HT/purC

    Plasmid
    #73623
    Purpose
    Produces Escherichia coli 5-aminoimidazole-4-N-succinocarboxamide ribonucleotide synthetase (PurC), N-terminal His6 tag
    Depositor
    James Williamson, T. J. Kappock
    Article
    Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.
    Insert
    phosphoribosylaminoimidazolesuccinocarboxamide synthase (purC Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Contains multiple silent mutations (E179, G185, G…
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73623-plasmid-map-sequence-id-136194

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  12. BCD2_BC

    Plasmid
    #66024
    Purpose
    MoClo Basic Part: RBS - BiCistronic Design, high strength (RBS part type, actually contains a small transcriptional unit and second RBS) [B:BCD2:C]
    Depositor
    Douglas Densmore
    Article
    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.
    Insert
    RBS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    66024-plasmid-map-sequence-id-114158

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  16. C0062 (luxR)_CD

    Plasmid
    #66028
    Purpose
    MoClo Basic Part: CDS - Controller protein, luxR repressor/activator (in concert with HSL, represses pLuxR(pR) R0063. Also up-regulates pLuxR(pL) R0062) [C:C0062:D]
    Depositor
    Douglas Densmore
    Article
    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.
    Insert
    Transcription factor
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    66028-plasmid-map-sequence-id-114215

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  20. R0040 (pTet)_AB

    Plasmid
    #66008
    Purpose
    MoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [A:R0040:B]
    Depositor
    Douglas Densmore
    Article
    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.
    Insert
    Controllable promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    66008-plasmid-map-sequence-id-114120

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  21. R0040 (pTet)_EB

    Plasmid
    #66009
    Purpose
    MoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [E:R0040:B]
    Depositor
    Douglas Densmore
    Article
    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.
    Insert
    Controllable promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    66009-plasmid-map-sequence-id-114123

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  22. R0040 (pTet)_FB

    Plasmid
    #66010
    Purpose
    MoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [F:R0040:B]
    Depositor
    Douglas Densmore
    Article
    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.
    Insert
    Controllable promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    66010-plasmid-map-sequence-id-114125

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  23. R0040 (pTet)_GB

    Plasmid
    #66011
    Purpose
    MoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [G:R0040:B]
    Depositor
    Douglas Densmore
    Article
    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.
    Insert
    Controllable promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    66011-plasmid-map-sequence-id-114127

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  25. pzs1[GalS-L]

    Plasmid
    #60743
    Purpose
    Contains PIq driving expression of GalS-R, the Fucose inducible chimera with the wt Lac DBD.
    Depositor
    Matthew Bennett
    Article
    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.
    Insert
    GalS-L
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    LacI/GalR repressor chimera. LacI DBD with GalS L…
    Promoter
    Available since
    July 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    60743-plasmid-map-sequence-id-97728

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  26. pZS1[RbsR-T]

    Plasmid
    #60751
    Purpose
    Contains PIq driving expression RbsR-T, the Ribose inducible chimera with the TAN DBD.
    Depositor
    Matthew Bennett
    Article
    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.
    Insert
    RbsR-T
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Chimeric LacI/GalR repressor with the TAN DBD and…
    Promoter
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    60751-plasmid-map-sequence-id-97732

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  27. pZS1[RbsR-L]

    Plasmid
    #60741
    Purpose
    Contains PIq driving expression RbsR-L, the Ribose inducible chimera with the wt Lac DBD.
    Depositor
    Matthew Bennett
    Article
    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.
    Insert
    RbsR-L
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    LacI/GalR repressor chimera. LacI DBD with RbsR L…
    Promoter
    Available since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    60741-plasmid-map-sequence-id-97726

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  28. sgFFL_d23

    Plasmid
    #59892
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with two seed sites mutated
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124–3
    Use
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--3rd and fourth seed site removed
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59892-plasmid-map-sequence-id-108249

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  29. sgFFL_d3

    Plasmid
    #59891
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with one seed site mutated.
    Depositor
    Georg Seelig
    Article
    Strovas et al ACS Synth Biol. 2014 May 16;3(5):324-31.
    Insert
    mCherry with intron containing the mouse mir-124–3
    Use
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--3rd seed site removed
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    59891-plasmid-map-sequence-id-108247

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  31. pAV10

    Plasmid
    #63213
    Purpose
    pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    63213-plasmid-map-sequence-id-113915

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  35. pcDNA C12 ecDHFR DD YFP HA

    Plasmid
    #192815
    Purpose
    Expresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.
    Depositor
    John Hulleman
    Article
    Nakahara et al ACS Chem Biol. 2022 Sep 19. doi: 10.1021
    Insert
    E. coli dihydrofolate reductase
    Use
    Tags
    enhanced yellow fluorescent protein and hemagglut…
    Expression
    Mammalian
    Mutation
    W74R/T113S/E120D/Q146L
    Promoter
    CMV
    Available since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192815-plasmid-map-sequence-id-377240

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  39. pDule2-Mb haloTyrRS C6

    Plasmid
    #160378
    Purpose
    C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.
    Depositor
    Ryan Mehl
    Article
    Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574
    Insert
    C6 HaloTyrosine tRNA synthatase
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    L270S, Y271L, N311G, C313T
    Promoter
    Available since
    Nov. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    160378-plasmid-map-sequence-id-315318

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  40. qTAG-N-Solo-mScarlet-TUBA1B

    Plasmid
    #207764
    Purpose
    Donor template for mScarlet insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a mScarlet Tag (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207764-plasmid-map-sequence-id-413790

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Showing: 6441 - 6480 of 19527 results