We narrowed to 9,404 results for: pho;

1. pGEX SHP-2(NC)-SH2

   Plasmid

   #46499

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   SHP-2(NC) (PTPN11 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1-222

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA 3.2/V5-DEST CDC42EP1 S192A

   Plasmid

   #69820

   Purpose

   Expresses CDC42EP1-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   CDC42 effector protein (CDC42EP1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Mutation

   S192A

   Promoter

   CMV

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-ARAP3

   Plasmid

   #39484

   Depositor

   Sonja Vermeren

   Article

   Krugmann et al Mol Cell. 2002 Jan;9(1):95-108.

   Insert

   ARAP3 (ARAP3 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Aug. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

   Plasmid

   #51248

   Purpose

   Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli3 (Gli3 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV and Flag

   Expression

   Mammalian

   Mutation

   amino acids 849,865,877,907,980,1006 mutated to A…

   Promoter

   EF1a

   Available Since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pBrain-ch-TOGKDP-GFP-shch-TOG

   Plasmid

   #69113

   Purpose

   Dual-promoter plasmid to express knockdown-proof human ch-TOG tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.

   Depositor

   Stephen Royle

   Article

   Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

   Inserts

   Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
   shch-TOG
   

   Use

   RNAi

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Silent mutations to confer shRNA resistance.

   Promoter

   CMV

   Available Since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase

   Plasmid

   #196185

   Purpose

   Co-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-His

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

   Inserts

   GSK3B (GSK3B Human)
   λPPase
   

   Tags

   GST, HA, His6, and MBP

   Expression

   Bacterial

   Mutation

   changed Serine 9 to Alanine

   Promoter

   Tac

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-DroshaS300E/S302D

   Plasmid

   #62531

   Purpose

   Serine 300 of Drosha is mutated to glutamic acid and serine 302 is mutated to aspartic acid

   Depositor

   Bharat Ramratnam

   Article

   Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

   Insert

   human Drosha with serine 300 changed to glutamic acid and serine 302 changed to aspartic acid (DROSHA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed serine 300 to glutamic acid and serine 3…

   Available Since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

8. mGFP-hNET

   Plasmid

   #193364

   Purpose

   expression of human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cells

   Depositor

   Harald Sitte

   Article

   Luethi et al Commun Biol. 2022 Nov 17;5(1):1259. doi:

   Insert

   human Norepinephrine Transporter (SLC6A2 Human)
   

   Tags

   monomeric GFP (mGFP)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBOBI C-FLAG PHGDH

   Plasmid

   #212995

   Purpose

   expresses human PHGDH

   Depositor

   Sheng-cai Lin

   Article

   Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

   Insert

   PHGDH (PHGDH Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Flag-hPKL WT

    Plasmid

    #107159

    Purpose

    For mammalian expression of Flag-hPKL WT

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    Flag-hPKL WT (PKLR Human)
    

    Use

    Retroviral

    Tags

    N-term Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcdna6.2- myc ULK1 wt

    Plasmid

    #27629

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse myc ULK1 (Myc Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Available Since

    Feb. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pGEX4T2-Ste7-EE

    Plasmid

    #52681

    Purpose

    expression of constitutively active Ste7 kinase in bacteria

    Depositor

    Benjamin Turk

    Article

    Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi

    Insert

    Ste7 (STE7 Budding Yeast)
    

    Tags

    GST and Myc

    Expression

    Bacterial

    Mutation

    S359E/T363E constitutively active

    Promoter

    tac

    Available Since

    April 30, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pFl Strep-sumo-TEV-hAgo2 D597N

    Plasmid

    #136236

    Purpose

    Expressing catalytically inactive human Argonaute-2 in insect cells

    Depositor

    Leemor Joshua-Tor

    Article

    Elkayam et al Nucleic Acids Res. 2017 Apr 7;45(6):3528

    Insert

    human Argonaute-2 (AGO2 Human)
    

    Tags

    Sterp Sumo

    Expression

    Insect

    Mutation

    D597N

    Promoter

    polyhedrin

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX SHP-2(N)-SH2

    Plasmid

    #46498

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHP-2(N) (PTPN11 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-104

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX RASGAP(N)-SH2

    Plasmid

    #46432

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Gap/RASGAP(N) (RASA1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 168-283

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLJM1-FH-AGO2-5xA

    Plasmid

    #91979

    Purpose

    Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S824A, S828A, T830A, S831A, S834A (5xA)

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX SHP-2(C)-SH2

    Plasmid

    #46497

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHP-2(C) (PTPN11 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 101-222

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MSCV-Puro-FH-AGO2-5xE

    Plasmid

    #91993

    Purpose

    Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Retroviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S824E, S828E, T830E, S831E, S834E (5xE)

    Available Since

    Jan. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLJM1-FH-AGO2-5xE

    Plasmid

    #91981

    Purpose

    Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S824E, S828E, T830E, S831E, S834E (5xE)

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR221 - myc ULK1 wt

    Plasmid

    #27623

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse myc ULK1 (Myc Mouse)
    

    Use

    Gateway donr vector

    Tags

    myc

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Available Since

    Feb. 18, 2011

    Availability

    Academic Institutions and Nonprofits only