Skip to main content
Addgene

We narrowed to 8,877 results for: BLI;

Showing: 8101 - 8150 of 8877 results
  1. pHAGE2-TetO-ePOUSKM

    Plasmid
    #206398
    Purpose
    Expresses four genes (human ePOU, SOX2, KLF4, c-MYC) in mammalian cells, for lentivirus generation.
    Depositor
    Insert
    ePOU, SOX2, KLF4, c-MYC (KLF4, MYC, SOX2 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. LgBiT-CYBC1:CYBB-SmBiT BiBiT Vector(CMV)

    Plasmid
    #237084
    Purpose
    Express LgBiT-CYBC1:CYBB-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter
    Has Service
    DNA
    Inserts
    CYBC1 (CYBC1 Human)
    CYBB (CYBB Human)
    Use
    Tags
    LgBiT and SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  3. NanoBRET PD-1/SHP2 Bidirectional Vector

    Plasmid
    #237016
    Purpose
    Express NanoLuc(R)-PD1/SHP2-HaloTag Fusion Proteins in Mammalian Cells under a CMV promoter
    Has Service
    DNA
    Inserts
    PD-1 (PTPN11 Human)
    SHP2 (PTPN11 Human)
    Use
    Tags
    HaloTag (R) and NanoLuc (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  4. NanoLuc-NRAS (Q61R)-CRAF RBD-HaloTag BiBRET Vector

    Plasmid
    #236862
    Purpose
    Express NanoLuc(R)-NRAS (Q61R)-CRAF RBD-HaloTag(R) in Mammalian Cells under a CMV promoter
    Has Service
    DNA
    Inserts
    NRAS (NRAS Human)
    cRAF(RBD) (RAF1 Human)
    Use
    Tags
    HaloTag (R) and NanoLuc (R)
    Expression
    Mammalian
    Mutation
    Q61R
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  5. BiBRET Vector HaloTag-NRAS (Q61R)-BRAF RBD-NanoLuc

    Plasmid
    #236852
    Purpose
    Express HaloTag(R)-NRAS (Q61R)-BRAF RBD-NanoLuc(R) in Mammalian Cells under a CMV promoter
    Has Service
    DNA
    Inserts
    NRAS (NRAS Human)
    BRAF (RBD) (BRAF Human)
    Use
    Tags
    HaloTag (R) and NanoLuc (R)
    Expression
    Mammalian
    Mutation
    Q61R
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  6. pLV-SFFV-CKAP4 C100A-WPRE-UbC-Emerald

    Plasmid
    #225949
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutation C100A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    C100A
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. pLV-SFFV-CKAP4 CC-WPRE-UbC-Emerald

    Plasmid
    #225950
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutation double cysteine (CC) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Additional cysteine inserted after Cys-100
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. pLV-SFFV-CKAP4(1-131)-WPRE-UbC-Emerald

    Plasmid
    #225955
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutant lacking the luminal domain under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Truncated CKAP4 (1-131) lacking the luminal domain
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. pLV-SFFV-RTN4-WPRE-UbC-Emerald

    Plasmid
    #225938
    Purpose
    Lentiviral vector plasmid expressing human reticulon 4 (RTN4) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Inserts
    RTN4 (RTN4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
  10. pLV-SFFV-myc-STIM2-WPRE-UbC-Emerald

    Plasmid
    #225940
    Purpose
    Lentiviral vector plasmid expressing human stromal interaction molecule 2 (STIM2) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Inserts
    STIM2 (STIM2 Human)
    Emerald
    Use
    Lentiviral
    Tags
    myc-tag
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. pSCBE3-HF1

    Plasmid
    #218156
    Purpose
    This plasmid harbors the base editor SCBE3-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.
    Depositor
    Inserts
    scbe3-HF1
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Tags
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N497A…
    Promoter
    rpsL promoter
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  12. pSCBE3-HF1-Hypa

    Plasmid
    #218158
    Purpose
    This plasmid harbors the base editor SCBE3-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.
    Depositor
    Inserts
    scbe3-HF1-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Tags
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N497A…
    Promoter
    rpsL promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
  13. pSCBE3-Hypa

    Plasmid
    #218157
    Purpose
    This plasmid harbors the base editor SCBE3-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.
    Depositor
    Inserts
    scbe3-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Tags
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N692A…
    Promoter
    rpsL promoter
    Available since
    May 10, 2024
    Availability
    Academic Institutions and Nonprofits only
  14. B52_puro_gRNA_3418711_KCNB2

    Plasmid
    #197558
    Purpose
    gRNA targeting upstream region of KCNB2 (site 3418711, control for epigenetic targeting)
    Depositor
    Insert
    upstream KCNB2 promoter gRNA (Kcnb2 Rat)
    Use
    Grna with puromycin selection
    Tags
    puromycin resistance cassette under CMV promotion
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  15. pGL3-pSMANTIS_SMAD-FOS_Mutant

    Plasmid
    #194188
    Purpose
    Includes the promoter (1kb) of SMTS with mutated SMAD/FOS binding site
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated SMAD/FOS Site, Region 3-15nt of 1000nt
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  16. pGL3-pSMANTIS_STAT1-2_Mutant

    Plasmid
    #194189
    Purpose
    Includes the promoter (1kb) of SMTS with mutated STAT1/2 binding site
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    mutated STAT1/2 site, Region 653-673nt of 1000nt
    Promoter
    SMANTIS promoter (1kb), mutated
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  17. deltaVP1 + VP2C-GFP-MIOX

    Plasmid
    #166682
    Purpose
    Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Inserts
    VP2C-GFP-MIOX
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    April 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  18. MIOX only

    Plasmid
    #166681
    Purpose
    Yeast integrative plasmid for expressing mouse myo-inositol oxygenase (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Insert
    MIOX (Miox Budding Yeast, Synthetic, Mouse)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL10
    Available since
    April 9, 2021
    Availability
    Academic Institutions and Nonprofits only
  19. wtVP1 + VP2C-GFPdeg

    Plasmid
    #166678
    Purpose
    Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Inserts
    VP2C-GFPdeg
    Wild-type Murine polyomavirus VP1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  20. deltaVP1 + VP2C-GFPdeg

    Plasmid
    #166677
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Inserts
    VP2C-GFPdeg
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  21. pQC V5 mKO2 HuR.DelHNS IRES Puro

    Plasmid
    #110389
    Purpose
    γ-Retroviral transfer vector for expressing HuR (delHNS), V5 and mKO2 tags, IRES-driven Puromycin selection.
    Insert
    ELAV like RNA binding protein 1 (ELAVL1 Human)
    Use
    Retroviral
    Tags
    V5 and mKO2
    Expression
    Mammalian
    Mutation
    HNS (HuR nuclear-cytoplasmic shuttling sequence) …
    Promoter
    CMV
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
  22. pJC20cdc8.110

    Plasmid
    #99363
    Purpose
    Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.110.
    Depositor
    Insert
    cdc8 (cdc8 Fission Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Alanine 18 to Threonine and Glutamic acid 31 to L…
    Promoter
    T7
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
  23. pJK215

    Plasmid
    #72427
    Purpose
    Acetobacter aceti 1023 gDNA
    Depositor
    Inserts
    succinyl-diaminopimelate desuccinylase
    2,3,4,5-tetrahydropyridine-2,6-carboxylate N-succinyltransferase
    acetylglutamate kinase
    ribosome biogenesis GTP-binding protein YsxC
    insertase
    ribonuclease P
    50S ribosomal protein L34
    hypothetical protein
    glyoxylase I
    hypothetical protein
    UDP-N-acetylglucosamine acyltransferase
    3-hydroxyacyl-ACP dehydratase
    UDP-3-O-(3-hydroxymyristoyl) glucosamine N-acyltransferase
    Use
    Phage lambda cloning vector with genomic dna inse…
    Tags
    Expression
    Mutation
    missing C-terminal domain residues 277-391(ter) a…
    Promoter
    n/a
    Available since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
  24. kiCAP-AAV-MyoAAV2A

    Plasmid
    #224440
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 2A, a muscle-tropic AAV capsid in mice.
    Depositor
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDQTTL insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  25. kiCAP-AAV-MyoAAV4A

    Plasmid
    #224448
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 4A, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDYNSL insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
  26. kiCAP-AAV-MyoAAV1A

    Plasmid
    #224437
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 1A, a muscle-tropic AAV capsid in mice.
    Depositor
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDLTTP insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  27. kiCAP-AAV-MyoAAV3A

    Plasmid
    #224442
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 3A, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDYVGL insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  28. pMSCVpuro-Mito-Pericam

    Plasmid
    #87381
    Purpose
    Retroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.
    Depositor
    Insert
    Mito-Pericam [ratiometric]
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Retroviral 5' LTR promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
  29. kiCAP-AAV-MyoAAV4E

    Plasmid
    #224452
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 4E, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDFNNT insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
  30. PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

    Plasmid
    #166693
    Purpose
    Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.
    Depositor
    Inserts
    dCas9-VPR
    3x Cnga1 promoter-targeting sgRNAs
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE and U6
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
  31. kiCAP-AAV-MyoAAV1E

    Plasmid
    #224439
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 1E, a muscle-tropic AAV capsid in mice.
    Depositor
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDTMSK insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  32. kiCAP-AAV-MyoAAV3F

    Plasmid
    #224447
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 3F, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDHASW insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 8101 - 8150 of 8877 results