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We narrowed to 22,820 results for: Fus

Showing: 8101 - 8150 of 22820 results

  5. pME-mKate2-KDEL-pA

    Plasmid
    #109546
    Purpose
    Multisite gateway vector for expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence (marks the ER, or ER/SR in muscle)
    Depositor
    Rob Parton
    Article
    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:
    Insert
    mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence
    Use
    Zebrafish plasmids
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    109546-plasmid-map-sequence-id-223868

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  11. pV92.2

    Plasmid
    #78409
    Purpose
    pKT230 plasmid engineered with the RTCas1v2-GFP cassette (RT-Cas1, Cas2, GFP) driven by the MMB-1 16S rRNA promoter, the CRISPR03 array driven by its native leader, and the tmRNA::td intron construct
    Depositor
    Andrew Fire
    Article
    Silas et al Science. 2016 Feb 26;351(6276):aad4234.
    Inserts
    RT-Cas1
    Cas2
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    78409-plasmid-map-sequence-id-146291

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  17. pFEndoNA

    Plasmid
    #174724
    Purpose
    Expresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.
    Depositor
    Jukka Finne
    Article
    Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149
    Insert
    GFP-endoNA fusion protein
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    174724-plasmid-map-sequence-id-343066

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  20. pBbB8k-csg-amylase

    Plasmid
    #166859
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to α-amylase via a flexible linker. Derived from https://www.addgene.org/35363.
    Depositor
    Neel Joshi
    Article
    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469
    Insert
    csgBACEFG, with α-amylase fused to CsgA via flexible linker
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    166859-plasmid-map-sequence-id-329647

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  23. FRB_nSuMMVp

    Plasmid
    #118970
    Purpose
    Expresses N-terminus of sunflower mild mosaic virus protease (SuMMVp) fused to the FKBP-rapamycin binding (FRB) domain
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split nSuMMVp in fusion with FRB
    Use
    Tags
    Myc tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    118970-plasmid-map-sequence-id-231812

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  24. FKBP_cSuMMVp

    Plasmid
    #119182
    Purpose
    Expresses C-terminus of sunflower mild mosaic virus protease (SuMMVp) fused to the FK-506 binding protein (FKBP)
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split cSuMMVp in fusion with FKBP
    Use
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119182-plasmid-map-sequence-id-231824

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  29. SnFR-gamma8

    Plasmid
    #165496
    Purpose
    Green fluorescent glutamate reporter fused to TARP gamma-8 for mammalian expression.
    Depositor
    Andrew Plested
    Article
    Hao et al eLife. 2023 Jan 9;12:e84029. doi: 10.755
    Insert
    iGluSnFR extracellular domain fused to gamma-8 via NETO2 TM domain (Cacng8 Synthetic, Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    165496-plasmid-map-sequence-id-327724

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  30. pcDNA3-Nrxn1beta AS4(-)-Fc

    Plasmid
    #59313
    Purpose
    Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]
    Depositor
    Peter Scheiffele, Tito Serafini
    Article
    Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.
    Insert
    Nrxn1beta AS4(-)-Fc fusion (Nrxn1 Mouse)
    Use
    Tags
    Fc (from human IgG)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    June 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    59313-plasmid-map-sequence-id-107095

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  31. pR1G108CP20X

    Plasmid
    #85086
    Purpose
    Expresses PCNA1 variant (G108C) fused to putidaredoxin reductase and putidaredoxin in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi
    Insert
    The G108C variant of PCNA1 fused to putidaredoxin reductase and putidaredoxin
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    G108C in PCNA1
    Promoter
    T7 promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    85086-plasmid-map-sequence-id-164463

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  36. pcDNA3-Nrxn1beta AS4(+)-Fc

    Plasmid
    #59314
    Purpose
    Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]
    Depositor
    Peter Scheiffele, Tito Serafini
    Article
    Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.
    Insert
    Nrxn1beta AS4(+)-Fc fusion (Nrxn1 Mouse)
    Use
    Tags
    Fc (from human IgG)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    June 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    59314-plasmid-map-sequence-id-107094

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  44. ABI_cPPVp

    Plasmid
    #119212
    Purpose
    Expresses C-terminus of plum pox virus protease (PPVp) fused to the ABI-abscisic acid responsive protein domain
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split cPPVp in fusion with ABI
    Use
    Tags
    myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    119212-plasmid-map-sequence-id-231816

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  45. PYL1_nPPVp

    Plasmid
    #119211
    Purpose
    Expresses N-terminus of plum pox virus protease (PPVp) fused to PYL1-abscisic acid responsive protein domain
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split nPPVp in fusion with PYL1
    Use
    Tags
    AU1 tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119211-plasmid-map-sequence-id-232035

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  47. pPBO.ACT005

    Plasmid
    #182715
    Purpose
    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region
    Depositor
    Joseph Schoeniger
    Article
    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666
    Insert
    CasRx crRNA cloning backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Catalytically deactivated R295A, H300A, R849A, H8…
    Promoter
    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)
    Available since
    Aug. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    182715-plasmid-map-sequence-id-361296

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  48. pASPIre2

    Plasmid
    #154843
    Purpose
    Derivative of pASPIre1 carrying a bxb1-sfGFP translational fusion in place of bxb1
    Depositor
    Markus Jeschek
    Article
    Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:
    Insert
    Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    154843-plasmid-map-sequence-id-307634

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Showing: 8101 - 8150 of 22820 results