We narrowed to 4,881 results for: phy

1. pGL4-UPRE-luc2P-Hygro

   Plasmid

   #101788

   Purpose

   Luciferase reporter plasmid containing three tandem repeats of the unfolded protein response element (UPRE)

   Depositor

   Seiichi Oyadomari

   Article

   Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

   Insert

   3xUPRE
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   minimal TATA-box promoter with low basal activity

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDawn-Ag43

   Plasmid

   #107742

   Purpose

   Expresses Ag43 under the control of a blue-light transcriptional regulator

   Depositor

   Ingmar Riedel-Kruse

   Article

   Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

   Insert

   Ag43 (flu )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   removed all pstI cut sites with silent mutations,…

   Promoter

   pLambda

   Available since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB_tre_dCas9_KRAB

   Plasmid

   #126030

   Purpose

   PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Inserts

   dCas9 fusion with KRAB
   Hygromycin resistance
   

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   EF1 alpha and TRE

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLM494

   Plasmid

   #100539

   Purpose

   CEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.

   Depositor

   Bernd Müller-Röber

   Article

   Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:

   Inserts

   GGPP synthase
   phytoene desaturase
   phytoene-beta carotene synthase
   HMG CoA reductase
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PGK1, TDH3, TIP1, and ZEO1

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB_tre_dCas9_VP160

   Plasmid

   #126031

   Purpose

   PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Inserts

   dCas9 fusion with VP160
   Hygromycin resistance
   

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   EF1 alpha and TRE

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-PfV-Sapphire-IRES-DSRed

   Plasmid

   #116934

   Purpose

   Mammalian lenti vector for expression of the 40nm GEM and DSRed

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   Pfv
   

   Use

   Lentiviral

   Tags

   Sapphire

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHLmMBP-3

   Plasmid

   #72345

   Purpose

   Mammalian expression of secreted N-terminally 8His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

   Depositor

   Luca Jovine

   Article

   Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

   Type

   Empty backbone

   Use

   Tags

   6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer + chicken beta-actin promoter

   Available since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3.1-pCMV-PfV-GS-Sapphire

   Plasmid

   #116933

   Purpose

   Mammalian vector for expression of the 40nm GEM

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   Pfv
   

   Use

   Tags

   Sapphire

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. p4XBRE-SBE-SV40-GL3

   Plasmid

   #67811

   Purpose

   luciferase reporter with mouse Id1 promoter fragments

   Depositor

   Ron Prywes

   Article

   Lewis et al Biochim Biophys Acta. 2013 Oct;1829(10):

   Insert

   Id1 (Id1 Mouse)
   

   Use

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Id1/SV40

   Available since

   Nov. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

10. BCAM promoter-pRB-Puro-TurboRFPmyc-T2A-Luciferase

    Plasmid

    #188391

    Purpose

    Reporter for BCAM expression

    Depositor

    Markus Frank

    Article

    Sasamoto et al Cell Rep. 2022 Aug 9;40(6):111166. doi:

    Insert

    BCAM reporter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1-puro_CMV_NES-Caprola_06-mEGFP

    Plasmid

    #194693

    Purpose

    CMV driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through through lentivirus transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_06-mEGFP
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1-puro_CMV_NES-Caprola_off-mEGFP

    Plasmid

    #194695

    Purpose

    CMV driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through lentivirus transduction

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_off-mEGFP
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. plenti2-EF1a-ChR2-EYFP-WRPE

    Plasmid

    #125255

    Purpose

    3rd gen lentiviral expression of ChR2 with mutation fused to EYFP driven by EF1a promoter for optogenetic activation

    Depositor

    Roger D. Kamm

    Article

    Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi

    Insert

    hChR2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-SFPQ

    Plasmid

    #166959

    Purpose

    Expresses HA-tagged SFPQ protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ (SFPQ Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAD-KPS12-Pman

    Plasmid

    #133832

    Purpose

    Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    mKate(KPS12)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pman

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLJC6-HK2-3xFLAG

    Plasmid

    #163451

    Purpose

    lentiviral overexpression vector for human HK2

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    HK2 (HK2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    See: comments

    Promoter

    Ubc

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3/Myc-DNMT3A

    Plasmid

    #35521

    Purpose

    Mammalian expression of human DNMT3A with myc tag

    Depositor

    Arthur Riggs

    Article

    Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

    Insert

    DNMT3A (DNMT3A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. p23-NES-PguCas13b-msfGFP-NES-Flag

    Plasmid

    #165072

    Purpose

    overexpression of PguCas13b in human cells

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    PguCas13b
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA5/FRT_CMV_NES-Caprola_05-mEGFP

    Plasmid

    #194683

    Purpose

    CMV driven expression of the calcium recorder Caprola_05 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_05-mEGFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRPN265NB-enh-MNase

    Plasmid

    #166035

    Purpose

    Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

    Depositor

    Marc Timmers

    Article

    Nizamuddin et al Nucleic Acids Res. 2021 May 21;49(9):e49

    Insert

    Glutathione-S-Transferase/enhancer/MNase
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    lac promoter / tac promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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