We narrowed to 9,671 results for: SUB;
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Plasmid#182822PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiFluoxSnFR_PM
UseAAVPromoterhSynAvailable SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV9-hSyn-iFluoxSnFR_cyto
Plasmid#182823PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiFluoxSnFR_cyto
UseAAVPromoterhSynAvailable SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iEscSnFR_ER
Plasmid#182812PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiEscSnFR_ER
ExpressionMammalianPromoterhCMVAvailable SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-shRIIα
Plasmid#183454PurposesgRNA targeting rat PKA-RIIα subunitsDepositorInsertsgRNA targeting rat PKA-RIIα (Prkar2a Rat)
UseLentiviralPromotershRNA: H1 / gene: ubiquitinAvailable SinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX_HA-C7orf26v2
Plasmid#178536PurposeSubcloned C7orf26v2 (ENST00000359073, Broad GPP TRCN0000477172) into pLX_HA backboneDepositorAvailable SinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
p8322 pDONR ATG-YAP1 S94A-Stop
Plasmid#184526PurposeSubcloning YAP1 S94ADepositorAvailable SinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
p8321 pDONR ATG-YAP1 S127A-Stop
Plasmid#184525PurposeSubcloning YAP1 S127ADepositorAvailable SinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX_HA-C7orf26v1
Plasmid#178535PurposeSubcloned C7orf26v1 (ENST00000344417, Origene, RC219786L4) into pLX_HA backboneDepositorAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2601
Plasmid#175085PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions S47A, S65A, T75A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
ExpressionMammalianMutationS47A, S65A, T75AAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
iDianiSnFR
Plasmid#177741PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiDianiSnFR
TagsHA, His, and MycExpressionBacterialPromoterT7Available SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
iCytSnFR
Plasmid#177738PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiCytSnFR
TagsHA, His, and MycExpressionBacterialPromoterT7Available SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
iCyt_BrEt_SnFR
Plasmid#177740PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiCyt_BrEt_SnFR
TagsHA, His, and MycExpressionBacterialPromoterT7Available SinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
iCyt_F_SnFR
Plasmid#177739PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertiCyt_F_SnFR
TagsHA, His, and MycExpressionBacterialPromoterT7Available SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-EGFP-RanGAP*
Plasmid#175238PurposeBacterial expression and purification, low affinity SUMOylation substrate that can recruited to FRB with rapamycin, point mutation F562A reduces affinity for E2, increasing the KmDepositorAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCCM’ CbbQ-
Plasmid#162711PurposeSecond CCM operon with inactive CbbQ (K46A, E107Q) rubisco activaseDepositorInsertSecond CCM operon cloned from H. neapolitanus
ExpressionBacterialMutationInactivation of CbbQ subunit of CbbOQ rubisco act…PromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tac(5A)-GFP
Plasmid#162495PurposeExpresses C-terminally GFP tagged Tac with mutated five O-glycosylation sites in mammalian cellsDepositorInsertTac with five threonine residues mutated into alanine residues in the stem region (IL2RA Human)
TagsGFPExpressionMammalianMutationThe five threonine residues are mutated into alan…Available SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Tac(5A)
Plasmid#162493PurposeExpresses GFP tagged Tac with mutated five O-glycosylation sites in mammalian cellsDepositorInsertTac with five threonine residues mutated into alanine residues in the stem region (IL2RA Human)
TagsGFPExpressionMammalianMutationThe five threonine residues are mutated into alan…Available SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMinDis.X513-iNicSnFR3b-(V7)-ER
Plasmid#125123PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotineDepositorInsertiNicSnFR3a-(V7)-ER
TagsFusion protein -Endoplasmic retention motifExpressionMammalianPromoterT7Available SinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHHM.X513-iNicSnFR3a-(CC93)
Plasmid#124880PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotineDepositorInsertiNicSnFR3a-(CC93)
TagsHA Tag and His TagExpressionBacterialMutationNAPromoterT7Available SinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHHM.X513-iNicSnFR2-(CC90)
Plasmid#124836PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotineDepositorInsertiNicSnFR2-(CC90)
TagsHA tags and His TagExpressionBacterialMutationNAPromoterT7Available SinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19_T790M_28bp
Plasmid#114721Purpose28 bp fragment of the EGFR T790M allele used as SiMREPS analyte standardDepositorInsertEGFR (EGFR Human)
ExpressionBacterialMutationc.2369C>T (Substitution, position 2369, C➞T); …Available SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19_4xT790M_28bp
Plasmid#114719Purpose4 x 28 bp repeats of the EGFR T790M allele used as SiMREPS analyte standardDepositorInsertEGFR (EGFR Human)
ExpressionBacterialMutationc.2369C>T (Substitution, position 2369, C➞T); …Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAK4-FL-3'UTR-MT2
Plasmid#113093PurposeLuciferase reporter containing PAK4-FL- 3' UTR with the second predicted MiR-199a-3p binding site mutatedDepositorInsertp21 Activated kinase 4 (PAK4 Human)
UseLuciferaseExpressionMammalianMutationSecond predicted miR-199a-3p binding site mutated…PromoterPGKAvailable SinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
PAK4-FL-3'UTR-MT-both
Plasmid#113094PurposeLuciferase reporter containing PAK4-FL- 3' UTR with both the predicted MiR-199a-3p binding sites mutatedDepositorInsertp21 Activated kinase 4 (PAK4 Human)
UseLuciferaseExpressionMammalianMutationBoth the predicted miR-199a-3p binding site muta…PromoterPGKAvailable SinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_26kDa_Myc
Plasmid#74955PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 26kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTagsStrep II Tag (GGGGWSHPQFEKGGGGWSHPQFEK)ExpressionMammalianMutationdelta (1-62) M78A M80A M100A M128APromoterCMV, Lentiviral LTRAvailable SinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_33kda_Myc
Plasmid#74957PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 33kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTagsMyc tag (EQKLISEEDL)ExpressionMammalianMutationM63A M78A M80A M100A M128APromoterCMV, Lentiviral LTRAvailable SinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-20b-T7-Ntx-K49
Plasmid#58715PurposeExpresses in Escherichia coli T7-Notexin with the amino acid substitution D49KDepositorInsertNotexin
TagsT7 peptide tagExpressionBacterialMutationChanged Aspartic Acid 49 to LysinePromoterT7Available SinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
GoldyTALEN-ppp1cab TAL2
Plasmid#41836DepositorInsertprotein phosphatase 1, catalytic subunit, alpha isoform b (ppp1cab Zebrafish)
UseCloning vectorAvailable SinceFeb. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 BD (522-645, C643A)/pET28HMT
Plasmid#111073PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
TagsHis Tag and MBP tagExpressionBacterialMutationKv7.4 BD (522-645, C643A)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 (1-645, Δ368-492)/pcDNA3.1
Plasmid#111454PurposeElectrophysiology in HEK cellDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
ExpressionMammalianMutationKv7.4 (1-645, Δ368-492), C643AAvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-XRI-HA
Plasmid#178056PurposeExpression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with HA tag, under UBC promoter.DepositorInsertXRI-HA
UseAAVTagsHA-MBPExpressionMammalianPromoterHuman ubiquitin C (UBC) promoterAvailable SinceOct. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV_3XHA-ATP6V1B2-mNeonGreen
Plasmid#228865PurposeStable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreenDepositorAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-DsRed-MST-Kv2.1
Plasmid#131707PurposeExpresses Kv2.1in mammalian cellsDepositorAvailable SinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 hSCN5A
Plasmid#145374Purposeexpresses human Nav1.5 (WT) in mammalian cellsDepositorAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GGamma8-GFP2
Plasmid#140990PurposeEncodes a G gamma subunit (GNG8) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-FLAG-SF3B1-WT
Plasmid#82576PurposeExpresses a codon-optimized ORF of human SF3B1 (wild-type)DepositorAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 hSCN5A-L325R
Plasmid#145373Purposeexpresses mutant form of human Nav1.5 (L325R) found in patient with Brugada syndrome in mammalian cellsDepositorInsertsodium voltage-gated channel alpha subunit 5 (SCN5A Human)
ExpressionMammalianMutationchanged Leucine 325 to ArgininePromoterCMVAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-E1MLS-2×FLAG-T4Lig-IRES-tdTomato
Plasmid#234952PurposeHuman codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmidDepositorInserthuman codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
UseLentiviralTagsFLAGExpressionMammalianPromoterEF1aAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
WT Smarca4-sfGFP
Plasmid#107056PurposeExpression of wild-type Smarca4 (Brg1) with C-terminal super-folder GFP fusion.DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
UseLentiviralTagsSuper-folder GFP (sfGFP)ExpressionMammalianMutationwild-typePromoterCAGAvailable SinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
miniABEMax V82G nSaCas9
Plasmid#135365PurposeExpression of SaCas9 nickase (D10A) with single evolved TadA monomer (ABEMax) with a V82G substitutionDepositorInsertminiABEMax V82G nSaCas9
UseCRISPRExpressionMammalianMutation(V82G)Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
K785R Smarca4-sfGFP
Plasmid#107057PurposeExpression of dominant-negative K785R Smarca4 (Brg1) with C-terminal super-folder GFP fusion.DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
UseLentiviralTagsSuper-folder GFP (sfGFP)ExpressionMammalianMutationK785RPromoterCAGAvailable SinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-E1MLS-2xMyc-Ku-IRES-Hygro
Plasmid#234950PurposeHuman codon optimized Ku (Mycobacterium) with N-terminal E1 MLS expressing plasmidDepositorInserthuman codon optimized Ku with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
UseLentiviralTagsMycExpressionMammalianPromoterEF1aAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-E1MLS-2xFLAG-LigD-IRES-tdTomato
Plasmid#234951PurposeHuman codon optimized LigD (Mycobacterium) with N-terminal E1 MLS expressing plasmidDepositorInsertHuman codon optimized LigD with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
UseLentiviralTagsFLAGExpressionMammalianPromoterEF1AAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pA-CBh.TP-CNOT8-V5H6.MCh.Puro
Plasmid#209947PurposeIn mammalian cells expresses TP fused to a CNOT8 with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.DepositorInsertsCCR4-NOT transcription complex subunit 8 (CNOT8 Human)
mCherry fluorescent protein fused to puromycin resistance marker
TagsTP (MS2 coat protein) and V5 epitope with H6 tagExpressionMammalianPromoterCBh and CMVAvailable SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR-ABCB1_STOP
Plasmid#221424PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertABCB1 (ABCB1 Human)
ExpressionMammalianAvailable SinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Beta3
Plasmid#140988PurposeEncodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST PKAKTRClover
Plasmid#59153PurposeLentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)DepositorAvailable SinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-PM-RA-BlastR
Plasmid#211708PurposeLentiviral expression of a ddFP subunit (RA) fused with the lipid modification motif of lymphocyte-specific protein tyrosine kinase (Lck) for plasma membrane (PM) targetting (PM-RA)DepositorAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-4eCOL2A1
Plasmid#97211PurposeFor cloning of a COL2A1-based, chondrogenesis-responsive promoter, and subcloning this insert into new vectorsDepositorInsert4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
UseVector is designed for cloning and generation of …PromoterCOL2A1 regulatory elementsAvailable SinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant SigmaR1-mNeonGreen a5mut (F196A L199A F200A L203A Y206A L210A L214A Y217A)
Plasmid#226578PurposeEncodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreenDepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
TagsmNeonGreenExpressionMammalianMutationF196A, L199A, F200A, L203A, Y206A, L210A, L214A, …Available SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only