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Plasmid#69302PurposeGateway entry vector with pcoCas9D10ADepositorInsertpcoCas9D10A (Plant codon-optimized)
UseCRISPRTags2XFLAGExpressionMutationD10A mutation; NLS fusionPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMLS256
Plasmid#73715PurposeSapTrap destination vector for building combined sgRNA expression + repair template vectorsDepositorInsertpU6::sgRNA
UseCRISPRTagsExpressionWormMutationSapI insertion sitePromoterC. elegans U6 snRNA pol III promoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
act-AsCpf1
Plasmid#73917Purposeexpression plasmid of AsCpf1DepositorInserthAsCpf1
UseTagsNLS-3xHAExpressionInsectMutationPromoteract5CAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
OA-1050E (Ubiq-CasRx)
Plasmid#132416PurposeExpress CasRx under Ubiquitin-63E promoterDepositorInsertCasRx
UseTagsExpressionInsectMutationPromoterUbiqAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AkCas12b-2AeGFP
Plasmid#121946PurposeMammalian expression, Genome editingDepositorInsertAkCas12b
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-2-2xTY1-V5
Plasmid#61257PurposeCRISPR-RMCE, second step attB plasmid, intron phase 2, exchange dsRed with 2xTY1 and V5 tags cassetteDepositorInsert2xTY1-V5
UseCRISPRTagsTY1 and V5ExpressionMutationPromoterAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-1-2xTY1-V5
Plasmid#61256PurposeCRISPR-RMCE, second step attB plasmid, intron phase 1, exchange dsRed with 2xTY1 and V5 tags cassetteDepositorInsert2xTY1-V5
UseCRISPRTagsTY1 and V5ExpressionMutationPromoterAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Puro.3xBsmBI
Plasmid#196709PurposeBackbone for cloning guide libraries with untranscribed barcodes and a tracrRNA of choiceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
BPK2014-AmCas12b
Plasmid#121951PurposeE. coli expression, protein purificationDepositorInsertAmCas12b
UseTags10xHis tagExpressionBacterialMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
TU6m-gRNAscaffHygroR
Plasmid#165485PurposeS. pyogenes Cas9 guide RNA expression in tilapia cellsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterOreochromis mossambicus U6Available sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
BPK2013-AkCas12b
Plasmid#121950PurposeE. coli expression, protein purificationDepositorInsertAkCas12b
UseTags10xHis tag and 3xHA tagExpressionBacterialMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP2367
Plasmid#160076PurposeZ. mobilis CRISPRi, promoter C, empty guide, sfGFPDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICSL11197
Plasmid#191784PurposeSpCas9 expression in plantsDepositorInsertAtUbi10::Cas9 +13 introns-T-E9
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
dSp-VP64-RTA
Plasmid#99675PurposeExpresses dSp Cas9 fused at C term. To VP64 and RTA activation domainsDepositorArticleInsertdCas9
UseTagsVP64-RTAExpressionMammalianMutationdead Cas9PromoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
Construct 7 - CMVp-Cas9-3xNLS-HSVpA
Plasmid#81247PurposeExpresses Cas9 fused to 3xNLS driven by CMV promoter. For mammalian cell expressionDepositorInsertCas9-3xNLS
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BsCas12b-2AeGFP
Plasmid#121948PurposeMammalian expression, Genome editingDepositorInsertBsCas12b
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAW212
Plasmid#113234PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->CCATCDepositorInsertsCas1
Cas2
Leader-repeat
UseTagsExpressionBacterialMutationGGTAG->CCATCPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_SIX4_iso1
Plasmid#128238PurposeDonor vector for 3' FLAG tag of human SIX4_iso1DepositorInsertSIX4_iso1 Homology arms
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330.iGFP1
Plasmid#66581PurposesgRNA for iGFPDepositorInsertiGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AmCas12b-2AeGFP
Plasmid#121947PurposeMammalian expression, Genome editingDepositorInsertAmCas12b
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330.LoxP
Plasmid#66586PurposesgRNA for LSLDepositorInsertLoxP sgRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330.iGFP2
Plasmid#66582PurposesgRNA for iGFPDepositorInsertiGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJMP2480
Plasmid#160080PurposeZ. mobilis CRISPRi, promoter C, empty guideDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHdCas9-mammalian
Plasmid#138415PurposeExpression of Himar-dCas9 in mammalian cellsDepositorInsertHimar-dCas9
UseTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330.iGFP5
Plasmid#66584PurposesgRNA for iGFPDepositorInsertiGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330.LoxPO
Plasmid#66585PurposesgRNA for LSLDepositorInsertLSL-Tomato
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFOXA3.1.0-gDNA
Plasmid#112417PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor FOXA3DepositorInsertFOXA3 (FOXA3 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCD294.9
Plasmid#113319PurposedCas9, PCP-SoxS_R93A, J109 scRNA.b1DepositorInsertdCas9, PCP-SoxS_R93A, J109 scRNA.b1_PP7
UseCRISPRTagsExpressionBacterialMutationR93APromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBC2101-reci
Plasmid#117170PurposeU6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 with NLSDepositorInsertreci_wtCas9 with SV40 NLS
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330.iGFP3
Plasmid#66583PurposesgRNA for iGFPDepositorInsertiGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQdCas12a.luxR(mut)-sggfp(C)
Plasmid#236043PurposeThe plasmid pQdCas12a.luxR(mut)-sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.DepositorInsertquorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
UseCRISPRTagsExpressionMutationPromoterquorum sensing promoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQdCas12a.luxR(mut)-sggfp(B)
Plasmid#236041PurposeThe plasmid pQdCas12a.luxR(mut)-sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.DepositorInsertquorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
UseCRISPRTagsExpressionMutationPromoterquorum sensing promoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pQdCas12a.luxR(mut)-sggfp(A)
Plasmid#236039PurposeThe plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.DepositorInsertquorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
UseCRISPRTagsExpressionMutationPromoterquorum sensing promoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDONOR 6colors
Plasmid#229974PurposebigMamAct assembled plasmid for 6colors cellular labellingDepositorInsertmito-mCherry, EYFP-tubulin, H2B iRFP720, GTS-mTagBFP, mTFP1-actin
UseCRISPRTagsExpressionMammalianMutationSee Depositor CommentsPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMRS-empty
Plasmid#220203Purposemini-Tn7 cloning vector - empty backboneDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpy
Plasmid#220184PurposesgRNA expression vector - promoterless backboneDepositorInsertpromoterless backbone
UseCRISPRTagsExpressionBacterialMutationPromoterpromoterlessAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyA
Plasmid#220185PurposesgRNA expression vector - pEM7 promoterDepositorInsertpEM7 promoter
UseCRISPRTagsExpressionBacterialMutationPromoterpEM7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyC
Plasmid#220187PurposesgRNA expression vector - pBG42 promoterDepositorInsertpBG42 promoter
UseCRISPRTagsExpressionBacterialMutationPromoterpBG42Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpyD
Plasmid#220188PurposesgRNA expression vector - pBG28 promoterDepositorInsertpBG28 promoter
UseCRISPRTagsExpressionBacterialMutationPromoterpBG28Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
dSp-p65 Full (1-261)
Plasmid#99654PurposeExpresses dSp Cas9 fused to p65 activation domainDepositorArticleInsertdCas9
UseTagsp65 activation domainExpressionMammalianMutationdead Cas9PromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
iF67 PB-Zim3-XTEN-dCas9-mScarlet-Bsd-Neo-mAmetrine
Plasmid#204732PurposeConstitutive CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
iF51 PB-Zim3-dCas9-mScarlet-Hygro-Neo-SnapTag
Plasmid#204725PurposeConstitutive CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCSh2-2300
Plasmid#170984PurposeEncodes sgRNA target 2300 and Kanamycin resistance transposon.DepositorInsertSh-CAST sgRNA 2300
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCSh3-2300
Plasmid#170985PurposeEncodes sgRNA target 2300 and Tetracycline resistance transposon.DepositorInsertSh-CAST sgRNA 2300
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRO16Sh5
Plasmid#180076PurposeExpression of ShCAST under control of Plac with pRO1600 origin.DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLacAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOC3 cloning template vector
Plasmid#183422PurposeCreON FlpOFF knock-in vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJMP2373
Plasmid#160079PurposeZ. mobilis CRISPRi, promoter F, empty guide, sfGFPDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2371
Plasmid#160078PurposeZ. mobilis CRISPRi, promoter E, empty guide, sfGFPDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2369
Plasmid#160077PurposeZ. mobilis CRISPRi, promoter D, empty guide, sfGFPDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2132
Plasmid#160075PurposeZ. mobilis CRISPRi, promoter B, empty guideDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only