We narrowed to 10,924 results for: Kars

1. pSLIK 3xFLAG-TWIST2 neo

   Plasmid

   #197936

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-tagged TWIST2

   Depositor

   Kevin Janes

   Article

   Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

   Insert

   TWIST2 (TWIST2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. asPink chromoprotein promoter-less

   Plasmid

   #118005

   Purpose

   For course in "Synthetic Biology: A Lab Manual"

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   RBS, asPink
   

   Use

   Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSP2262

   Plasmid

   #70703

   Purpose

   Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus dCas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   D10A & H557A in SaCas9

   Promoter

   T7

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pY018 (pcDNA3.1-hPdCpf1)

   Plasmid

   #69990

   Purpose

   Expresses humanized PdCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hPdCpf1
   

   Use

   CRISPR

   Tags

   NLS & 3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Pet15b-Endosomal Escape Control

   Plasmid

   #186552

   Purpose

   Negative Control confirming the need for a CPP peptide in mediating internalization

   Depositor

   Jean Gariepy

   Article

   Mohammed et al J Control Release. 2012 Nov 28;164(1):58

   Insert

   6H-ETA-GFP
   

   Use

   Tags

   6xHis and EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Pet15b-Internalization and Endosomal Escape

   Plasmid

   #186551

   Purpose

   Internalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cells

   Depositor

   Jean Gariepy

   Article

   Mohammed et al J Control Release. 2012 Nov 28;164(1):58

   Insert

   10R-8His-ETA-EGFP
   

   Use

   Tags

   8xHis and EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-FKBP-GFP-OPTN (2-119)

   Plasmid

   #208867

   Purpose

   Stably express GFP-tagged OPTN (2-119aa) for CID induced mitophagy

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   OPTN (OPTN Human)
   

   Use

   Tags

   FKBP-GFP

   Expression

   Mammalian

   Mutation

   aa 2-119 only

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEXL flag Smad3

   Plasmid

   #10920

   Depositor

   Bob Weinberg

   Article

   Liu et al Proc Natl Acad Sci U S A. 1997 Sep 30. 9

   Insert

   Smad3 (SMAD3 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pUb dRxCas13d + RxCas13d guide RNA

   Plasmid

   #176304

   Purpose

   Expresses catalytic dead RxCas13d and its associated guide RNA in Drosophila cells

   Depositor

   Jeremy Wilusz

   Article

   Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

   Insert

   RxCas13d
   

   Use

   Tags

   HA tag

   Expression

   Insect

   Mutation

   R239A, H244A, R858A, H863A

   Promoter

   Ubi-p63e

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5myc Rac1 wt

    Plasmid

    #37030

    Depositor

    Lawrence Kirschner

    Article

    Manchanda et al Oncogene. 2013 Jul 25;32(30):3491-9. doi

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

11. Lenti_CRISPRi_sgNeg1

    Plasmid

    #166998

    Purpose

    Lentiviral expression of negative control sgRNA for CRISPRi knockdown

    Depositor

    Gian-Paolo Dotto

    Article

    Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

    Insert

    Negative control sgRNA 1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-4T1-MBP-TEV-NAP1 (WT)

    Plasmid

    #208871

    Purpose

    For expression in E. coli of NAP1

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Use

    Tags

    MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSP1D1G1

    Plasmid

    #97325

    Purpose

    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

    Depositor

    Michael Marty

    Article

    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

    Insert

    MSP1D1G1
    

    Use

    Tags

    6xHis - TEV

    Expression

    Bacterial

    Mutation

    Addition of G residue after T25

    Promoter

    T7

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. TetO-FUW-mir124a

    Plasmid

    #61539

    Purpose

    Expresses mir124a in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    mir124a (MIR124-1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pAF0286 Ef1a-Cp36_attB-BFP acceptor

    Plasmid

    #193474

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Cp36_attB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-Flag-hsDicer (K70A)

    Plasmid

    #41589

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K70A

    Promoter

    CMV

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pSMP-G9A_4

    Plasmid

    #36334

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    G9A (EHMT2 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

18. SaLgCP

    Plasmid

    #164562

    Purpose

    Puro

    Depositor

    Junwei Shi

    Article

    Verma et al Nat Cell Biol. 2021 Jan 18. pii: 10.1038

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Flag-NLS-SaCas9-P2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for crRNA expression and EFS promoter…

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. uORF1-Mreinit- 36xMashTag-BFP-3xStop-24xPP7 (uORF-MashTag reporter)

    Plasmid

    #128609

    Purpose

    Expresses uORF1 -36xMashTag with MoonTag as readout for reinitiation, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Insert

    uORF1-36xMashTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Pcmv

    Available since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET51b-His-TEV-HaloTag7_dead_mt

    Plasmid

    #167267

    Purpose

    Overexpression of the dead variant (D106A) of HaloTag7 in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    HaloTag7
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    D106A

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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