We narrowed to 9,202 results for: sacs

1. Ubp8/PET32a

   Plasmid

   #26965

   Depositor

   Cynthia Wolberger

   Article

   Samara et al Science. 2010 May 21. 328(5981):1025-9.

   Insert

   Ubp8 (UBP8 Budding Yeast)
   

   Use

   Tags

   His6 and Trx

   Expression

   Bacterial

   Mutation

   TEV site is added t the 5' end and 4 alanine…

   Promoter

   Available since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pT-35

   Plasmid

   #16092

   Depositor

   Yoshiko Kikuchi

   Article

   Takahashi et al J Biol Chem. 2001 Dec 28. 276(52):48973-

   Insert

   SMT3 (SMT3 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

3. Mammalian_SaAID3S

   Plasmid

   #188651

   Purpose

   Expresses SaAID3S and gRNA in mammalian cells

   Depositor

   Keiji Nishida

   Article

   Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

   Inserts

   nSaCas9-AID3S--UGI
   Sa-sgRNA
   

   Use

   Tags

   UGI

   Expression

   Mammalian

   Mutation

   D10A for SaCas9

   Promoter

   EF-1a and H1

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Mammalian_SaAID2S

   Plasmid

   #188652

   Purpose

   Expresses SaAID2S and gRNA in mammalian cells

   Depositor

   Keiji Nishida

   Article

   Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

   Inserts

   AID2S-nSaCas9--UGI
   Sa-sgRNA
   

   Use

   Tags

   UGI

   Expression

   Mammalian

   Mutation

   D10A for SaCas9

   Promoter

   EF-1a and H1

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. M2371 his3::ADE2 Disruptor Converter

   Plasmid

   #51671

   Purpose

   Yeast marker swap plasmid for converting HIS3 to ADE2

   Depositor

   David Stillman

   Article

   Voth et al Yeast. 2003 Aug;20(11):985-93.

   Insert

   ADE2 (ADE2 Budding Yeast)
   

   Use

   Yeast marker swap

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADE2

   Available since

   July 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. KBB285

   Plasmid

   #185076

   Purpose

   RFA3-GFP fusion

   Depositor

   Ken Belanger

   Article

   Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

   Insert

   RFA3
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. KBB284

   Plasmid

   #185075

   Purpose

   RFA2-GFP fusion

   Depositor

   Ken Belanger

   Article

   Belanger et al J Biol Chem. 2004 Oct 15;279(42):43530-9

   Insert

   RFA2
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRS314-prp16-302

   Plasmid

   #89974

   Purpose

   prp16-302 allele in TRP plasmid

   Depositor

   Christine Guthrie

   Article

   Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

   Insert

   PRP16 (PRP16 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   prp16-302 allele (R456K and G691R)

   Promoter

   Available since

   Aug. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. YIplac211-msGFP2-STE13

   Plasmid

   #177106

   Purpose

   Expresses msGFP2-Ste13 in yeast cells

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

   Insert

   STE13 (STE13 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL2B -376/+64

    Plasmid

    #24945

    Depositor

    Stephen Smale

    Article

    Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

    Insert

    IL-10 promoter (Il10 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

11. 1025

    Plasmid

    #160235

    Purpose

    FlightN-TetR(OFF)-VP16-Sv40-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    Tetracycline repressor
    Flightin promoter
    

    Use

    Tags

    VP16

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5/FRT/TO HIS SSA1

    Plasmid

    #19543

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    SSA1 (SSA1 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYES2.1-cerMET17

    Plasmid

    #107443

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET17 (MET17 Budding Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CMV-dH3-YFP

    Plasmid

    #8694

    Depositor

    Kami Ahmad

    Article

    Janicki et al Cell 2004 Mar 5;116(5):683-98.

    Insert

    H3 (His3:CG31613 Fly)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Cox4-DsRed retrovirus

    Plasmid

    #23215

    Depositor

    David Chan

    Article

    Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

    Insert

    Cox4 (COX4 Budding Yeast)
    

    Use

    Retroviral

    Tags

    DsRed

    Expression

    Mutation

    Cox4 mitochondrial targeting sequence fused to Ds…

    Promoter

    Available since

    July 8, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pVW284

    Plasmid

    #139494

    Purpose

    Expression vector for the PP7-GFP protein in S. cerevisiae

    Depositor

    Serge Pelet

    Article

    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

    Insert

    pADH1 PP7∆FG-GFPenvy tCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ∆FG

    Promoter

    pADH1

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVW300

    Plasmid

    #139495

    Purpose

    Expression vector for the PP7-GFP protein in S. cerevisiae

    Depositor

    Serge Pelet

    Article

    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

    Insert

    pTEF PP7∆FG-GFPenvy tCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ∆FG

    Promoter

    pTEF

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL2B -688/+64

    Plasmid

    #24944

    Depositor

    Stephen Smale

    Article

    Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

    Insert

    IL-10 promoter (Il10 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pGL2B -78/+64

    Plasmid

    #24947

    Depositor

    Stephen Smale

    Article

    Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

    Insert

    IL-10 promoter (Il10 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pRS424-TUB1-Cterminal6xHis

    Plasmid

    #60387

    Purpose

    Expression of yeast alpha tubulin (TUB1)- Cterminal 6xHis using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB1 (TUB1 Budding Yeast)
    

    Use

    Tags

    6xHIS

    Expression

    Yeast

    Mutation

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pTH848-gst-disec

    Plasmid

    #89589

    Purpose

    Plasmid for bacterial expression of His-tagged GST encoded by non-preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase codon-dis-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Destination Vector

    Plasmid

    #125754

    Purpose

    Destination vector for assembling gRNA fragments in the second round of assembly.

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

    Insert

    pTDH3-GFPdropout-tTDH1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRS405 VAC8-RFP

    Plasmid

    #166841

    Purpose

    Expresses Vac8-RFP in yeasts

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

    Insert

    VAC8 (VAC8 Budding Yeast)
    

    Use

    Tags

    RFP

    Expression

    Yeast

    Mutation

    Promoter

    VAC8 promoter

    Available since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. D588 lys2::HIS3 Disruptor Converter

    Plasmid

    #51675

    Purpose

    Yeast marker swap plasmid for converting LYS2 to HIS3

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    HIS3 (HIS3 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pNM3649

    Plasmid

    #154110

    Purpose

    miniMos dual component RMCE landing site vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    loxP::SEC::loxP::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3
    

    Use

    Minimos

    Tags

    Expression

    Mutation

    Promoter

    not applicable

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MBP-TEV-VMA Model vector

    Plasmid

    #86590

    Purpose

    This can be used to make MBP-TEV-Cterm with SapI and PstI; and can also make N-term-VMA with NdeI and SapI

    Depositor

    Sharon Rozovsky

    Article

    Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

    Insert

    VMA intein
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Original SapI site from pMal was mutated out

    Promoter

    Tac

    Available since

    Feb. 24, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pYES2.1-pomMet17

    Plasmid

    #107453

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    Met17 (met17 Fission Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. YIplac211-STV1-iGFP

    Plasmid

    #177111

    Purpose

    Expresses Stv1-iGFP in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    STV1 (STV1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCfB2192

    Plasmid

    #67539

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae XII-2 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSec7-Halox2

    Plasmid

    #115425

    Purpose

    Express Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesis

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

    Insert

    Sec7-Halox2
    

    Use

    Tags

    HaloTag

    Expression

    Yeast

    Mutation

    Promoter

    Endogenous

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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