We narrowed to 14,439 results for: eng
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Plasmid#67853PurposeGFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residuesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP fused to ELP (VPGVG) repeated 96 times
TagsGFP fused to N-terminal of ELPExpressionMammalianPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRG235
Plasmid#64533PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cas12Max
Plasmid#195334Purposevector for encoding a human codon-optimized Cas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon-optimized Cas12Max
Tags3xFlagExpressionMammalianMutationN243RPromoterCBh, CMV, hU6Available sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
plentiCMV10x-sct-pp65-b2m-HLA-A0201
Plasmid#196513PurposeExpress single-chain pMHC with sequencing tag for 10x genomicsDepositorInsertsingle chain of a pp65 peptide, b2m and HLA A0201
UseLentiviralTagsNAExpressionMammalianMutationY84C, 10xgenomics compatible tagPromoterCMVAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHBS1501 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaRRM]
Plasmid#133325PurposeTo test the effect of sequence on TDP43 splicing activityDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTARDBP mutant (TARDBP Human)
ExpressionMammalianMutationDeletion of RRM in full-length TDP43Available sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ICUE4
Plasmid#181846PurposeFourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertICUE4 (RAPGEF3 Human)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationGlutamine 122 in Epac1 domain mutated to glutamat…PromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DENV4 Rluc Reporter GND mutant Replicon
Plasmid#118586PurposeFor use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
UseYeast-e. col shuttle vector prs424MutationThe conserved GDD in the NS5 polymerase gene muta…Available sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132-STU-Lb
Plasmid#138099PurposeGolden Gate entry vector for 2nd crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_49
Plasmid#109247PurposepSC101 var7 BbsI cloning vectorDepositorInsertsfGFP
ExpressionBacterialPromoterPJ23105Available sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PEmaxdeltaRNaseH-dualU6-Dnmt1-PE3-loxP
Plasmid#207862PurposeAAV genome encoding C-terminal PEmaxDRNaseH and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNADepositorInsertNpuC-Cterm-PEmaxDRNaseH-dualU6-Dnmt1-PE3-loxP
UseAAVMutationSee ManuscriptPromoterCbhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7
Plasmid#175526PurposeCo-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLifeact-HaloTag11 and Tomm20-HaloTag7
ExpressionMammalianMutationHaloTag11 = HaloTag7-M175WAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330-Axin1
Plasmid#162543PurposesgRNA targeting Axin1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgRNA targeting Axin1 (Axin1 Mouse)
ExpressionMammalianMutationNonePromoterU6Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EF1α-AausFP1-Z1
Plasmid#135614PurposeAausFP1 - bright green fluorescent protein expression from A. australis under EF1a-HTLV promoter in minimal backbone plasmid.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAausFP1
UseSynthetic BiologyExpressionMammalianPromoterEF1a-HTLVAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_10E
Plasmid#91209PurposeDirect Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers , Plant Selection: noneDepositorInsert35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers
UseCRISPRExpressionPlantAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBDEST.IP
Plasmid#79472PurposePromoterless, Sleeping Beauty-compatible Gateway destination vector, IRES PuromycinDepositorTypeEmpty backboneUsePromoterless gateway destination vectorPromoternoneAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGT-Ah5
Plasmid#122572PurposeMAGIC donor plasmid (Himar transposon with catP/sfGFP payload)DepositorInsertcatP/sfGFP
UseSynthetic BiologyExpressionBacterialAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGNW6
Plasmid#122093Purposesuicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cellsDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGNW4
Plasmid#122088Purposesuicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cellsDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGT-Ah9
Plasmid#122576PurposeMAGIC donor plasmid (Himar transposon with tetQ/sfGFP payload)DepositorInserttetQ/sfGFP
UseSynthetic BiologyExpressionBacterialAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-secAnxaV-NS
Plasmid#67718PurposeGateway entry clone containing secreted human AnxaV with no stop codonDepositorInsertsecAnxaV-NS
ExpressionMammalianAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by