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YEplac181-TYS1

Plasmid

#8629

Depositor

Jeff Strathern

Article

Malagon et al Genetics 2004 Mar;166(3):1215-27.

Insert

TYS1 (TYS1 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type

Available Since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only

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hsp70:GalT-RFP

Plasmid

#105966

Purpose

heat shock inducible transgenesis, Galactsyl transferase (trans-golgi marker)

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

GalT

Tags

mRFP1

Expression

Bacterial

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV/SB10ΔDDE

Plasmid

#24552

Depositor

Perry Hackett

Article

Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

Insert

Sleeping Beauty delta DDE

Expression

Bacterial

Mutation

removes 322 bp of the transposase-coding region

Promoter

CMV

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pBT239

Plasmid

#27494

Depositor

Liqun Luo

Article

Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

Insert

TRE-tdTomato-myc

Use

Mouse Targeting

Tags

Myc

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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YEplac181-TAF14

Plasmid

#8625

Depositor

Jeff Strathern

Article

Malagon et al Genetics 2004 Mar;166(3):1215-27.

Insert

TAF14 (TAF14 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type

Available Since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only

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pMODloxZeo3DAmp

Plasmid

#27182

Depositor

Yoh Wada

Article

Aoyama et al Nucleic Acids Res. 2005 . 33(5):e52.

Insert

lox-EM7p-Zeo-lox cassette flanked by Tn5 outer element

Use

In vitro transposion

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pattB-RfB2

Plasmid

#62296

Purpose

docking site transgenesis plasmid for Gateway Cloning and insect transgenesis

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Use

Unspecified

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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YEplac181-DST1

Plasmid

#8624

Depositor

Jeff Strathern

Article

Malagon et al Genetics 2004 Mar;166(3):1215-27.

Insert

DST1 (DST1 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type

Available Since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only

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YEplac181-SUA7

Plasmid

#8627

Depositor

Jeff Strathern

Article

Malagon et al Genetics 2004 Mar;166(3):1215-27.

Insert

SUA7 (SUA7 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type

Available Since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only

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C-SWAT SMASh

Plasmid

#182523

Purpose

C-SWAT compatible vector for C-terminal tagging with SMASh Degron. (Protease Cleavage Site: DEMEECSKHL).

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

SMASh Tag

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2+NmCitrine-ABCB1a

Plasmid

#34956

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCB1a

Use

Sea urchin, zebrafish, xenopus

Tags

mCitrine

Expression

Mammalian

Promoter

SP6

Available Since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only
YEplac181-SOH1

Plasmid

#8623

Depositor

Jeff Strathern

Article

Malagon et al Genetics 2004 Mar;166(3):1215-27.

Insert

SOH1 (SOH1 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type

Available Since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only

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YEplac181-RTS1

Plasmid

#8626

Depositor

Jeff Strathern

Article

Malagon et al Genetics 2004 Mar;166(3):1215-27.

Insert

RTS1 (RTS1 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type

Available Since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only

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pSAM_Ec

Plasmid

#102939

Purpose

Transposon mutagenesis and Tn-seq

Depositor

Matthew Mulvey

Article

Wiles et al PLoS Genet. 2013;9(8):e1003716. doi: 10.

Insert

mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase

Expression

Bacterial

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pYSEAP

Plasmid

#37326

Depositor

John Schiller

Article

Pastrana et al Virology. 2004 Apr 10;321(2):205-16.

Insert

SEAP

Expression

Mammalian

Promoter

EF1a

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pBJ053_MTB_jGCaMP7f

Plasmid

#209280

Purpose

Ubiquitously expresses jGCaMP7f in zebrafish via tol2 transgenesis or in vitro transcription of mRNA

Depositor

Adam Cohen

Article

Jia et al Nature. 2023 Oct;622(7981):149-155. doi:

Insert

jGCaMP7f

Use

Zebrafish expression, in vitro transcription

Promoter

actb2

Available Since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSG5-BALF2

Plasmid

#72629

Purpose

Mammalian expression vector for Epstein-Barr virus single-strand DNA binding protein

Depositor

S. Diane Hayward

Article

Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

Insert

BALF2

Expression

Mammalian

Mutation

S1028P and V1088A

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only
pTF101.3

Plasmid

#134772

Purpose

A binary plasmid containing double CaMV 35S promoter to drive bialophos resistance gene (bar) for plant transformation.

Depositor

Kan Wang

Article

Testroet et al In Vitro Cell.Dev.Biol.-Plant (2017) 53:

Type

Empty backbone

Expression

Plant

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP12042

Plasmid

#222354

Purpose

Tn7 integrative vector with PabstBR promoter expression cassette and aprR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Type

Empty backbone

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMJH65

Plasmid

#67273

Purpose

Conjugally transferable red recombinogenic plasmid

Depositor

Mark Liles

Article

Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

Insert

tetA

Use

Red recombinase, temperature sensitive

Expression

Bacterial

Promoter

tetA promoter of plasmid pACYC184 origin

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

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