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pBetI-E1

Plasmid

#74677

Purpose

BetI-E1 gate

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PTac-RBSE1-BetI-PBetI-YFP

Expression

Bacterial

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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Cas9m2

Plasmid

#47314

Purpose

Cas9 D10A+H840A

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m2

Use

CRISPR

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
Measles V-FLAG

Plasmid

#44909

Depositor

Curt Horvath

Article

Palosaari et al J Virol. 2003 Jul;77(13):7635-44.

Insert

MeV (P/V/C )

Tags

FLAG

Available Since

Sept. 20, 2013

Availability

Academic Institutions and Nonprofits only
pCWU6-xylR-E-lepB

Plasmid

#190725

Purpose

fine tune expression of type 1 signal peptidase under the control of xylose- and theophylline- inducible system

Depositor

Chenggang Wu

Article

Bibek et al bioRxiv. 2023 Apr 24:2023.04.24.538132.

Insert

xylR-E-lepB

Expression

Bacterial

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pEB1-coVenNB

Plasmid

#104019

Purpose

Plasmid encoding coVenNB

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

coVenNB

Use

Low copy

Expression

Bacterial

Mutation

Same protein sequence as Venus NB (codon optimize…

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Trex2

Plasmid

#79246

Purpose

Expression vector for Trex2, which is a non-processive 3' exonuclease.

Depositor

Jeremy Stark

Article

Bennardo et al PLoS Genet. 2009 Oct;5(10):e1000683. doi

Insert

Trex2 (Trex2 Mouse)

Expression

Mammalian

Promoter

pCAGGS

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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BB1_P_12_asn_mbfA

Plasmid

#90289

Purpose

BB1 promoter mbfA gene of Aspergillus niger between FS1 and FS2

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

mbfA

Expression

Bacterial

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-mEGFP

Plasmid

#103979

Purpose

Plasmid encoding mEGFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mEGFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET23-CBD-(-PP)-EGFP-H246,249D

Plasmid

#12598

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Type

Empty backbone

Tags

6xHis and EGFP

Expression

Bacterial

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
GGE083

Plasmid

#120732

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Auxotrophy marker for selection after transformation in Y. lipolytica (Marker_URA3)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

Marker_URA3

Use

Cloning/assembly plasmid

Available Since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pHES875

Plasmid

#89192

Purpose

Expresses mCherry under control of GAL1 promoter.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pGAL1-mCherry

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pDsRed-miR9 Sensor

Plasmid

#22742

Depositor

Lynn Hudson

Article

Lau et al J Neurosci. 2008 Nov 5. 28(45):11720-30.

Insert

microRNA 9 binding sites

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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GGE085

Plasmid

#120738

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_Tef)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_Tef

Use

Cloning/assembly plasmid

Available Since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-2A-TAL6-MSTN

Plasmid

#72597

Purpose

MSTN TALEN with 2A-GFP tagged

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

TALEN for MSTN

Use

TALEN

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mNeongreen-T2A-mCherry

Plasmid

#125698

Purpose

Produces equimolar expression of mNeongreen and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mNeongreen-T2A-mCherry

Expression

Yeast

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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LP539

Plasmid

#90203

Purpose

pCMV-myc with cDNA encoding HsTRAPPC8 aa residues 1-700

Depositor

Lotte Pedersen

Article

Verdier et al Methods Mol Biol. 2016;1454:15-33. doi:

Insert

TRAPPC8 (TRAPPC8 Human)

Tags

Myc

Expression

Mammalian

Mutation

aa residues 1-700

Promoter

CMV

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV200

Plasmid

#110719

Purpose

Lentiviral system for reduced ectopic expression.

Depositor

Kevin Dean, Reto Fiolka

Article

Dean et al Biophys J. 2016 Mar 29;110(6):1456-65. d

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-Smurf1 C699A

Plasmid

#13503

Depositor

Jeff Wrana

Article

Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Insert

SMURF1 (SMURF1 Human)

Tags

GST

Expression

Bacterial

Mutation

C699A: catalytically inactive

Available Since

Dec. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3-myc-p110α-CAAX

Plasmid

#39490

Depositor

Julian Downward

Article

Rodriguez-Viciana et al EMBO J. 1996 May 15;15(10):2442-51.

Insert

p110 (PIK3CA Bovine)

Tags

CAAX and myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2012

Availability

Academic Institutions and Nonprofits only
GGE002

Plasmid

#120739

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_GAPdh)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_GAPdh

Use

Cloning/assembly plasmid

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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