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Cherry-HA-KCC2

Plasmid

#104077

Purpose

To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)

Depositor

Igor Medyna

Article

Kahle et al EMBO Rep. 2014 Jul;15(7):766-74. doi: 10

Insert

KCC2 (b isoform) (Slc12a5 Rat)

Tags

mCherry-HA tag

Expression

Mammalian

Mutation

EcoR1 site in rat KCC2 was removed by silent muta…

Promoter

UbC

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pHRgfpLox

Plasmid

#12264

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

eGFP

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

EGFP, LoxP added to pHR' vector.

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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AND-gate-V2.0

Plasmid

#44449

Depositor

Drew Endy

Article

Bonnet et al Science. 2013 May 3;340(6132):599-603. d

Insert

AND-gate

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only

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pRSVBneoT

Plasmid

#22883

Depositor

James Pipas

Article

Srinivasan et al Mol Cell Biol. 1997 Aug . 17(8):4761-73.

Insert

Early region SV40

Expression

Mammalian

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-Tomato MEF2C

Plasmid

#89715

Purpose

Retroviral gene expression vector for MEF2C expression

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C (MEF2C Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-Aurora

Plasmid

#98167

Purpose

red-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression.

Depositor

Peter Hegemann

Article

Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

Insert

Synthetic construct Aurora gene

Tags

mCherry

Expression

Mammalian

Mutation

V59S, E83N, E90Q, E101S, V117R, E123S, P242R, A24…

Promoter

CMV (+enhancer)

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CamKII-GFP-P2A-bReaches-minWPRE

Plasmid

#118276

Purpose

Expresses EGFP and bReaChES under a minimal CamKII Promoter

Depositor

Dietmar Schmitz

Article

Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

Inserts

EGFP
bReaCHES

Use

AAV

Tags

P2A

Promoter

CamK II

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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p55-H2B-Dendra2

Plasmid

#80609

Purpose

Encodes H2B-Dendra2-fusion protein under the CAG promoter. Linearize using Asc1 and Not1 for mouse embryo injection.

Depositor

Periklis Pantazis

Article

Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

Insert

H2B-Dendra2 (H2BC21 Synthetic, Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCS-lpp0.2-K64A

Plasmid

#201789

Purpose

mutant of PadR; transcriptional regulator can respond to p-coumaric acid

Depositor

Yajun Yan

Article

Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

Insert

PadR (padR Bacillus subtilis)

Expression

Bacterial

Mutation

K64A

Available Since

July 12, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pAc5.1B-EGFP-DmDCP1

Plasmid

#21684

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

Decapping protein 1 (Dcp1 Fly)

Use

Drosophila expression

Tags

EGFP

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pAB076i3

Plasmid

#79198

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1-SF2

Plasmid

#99020

Purpose

Bacterial expression of SF2

Depositor

Honglin Chen

Article

Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

Insert

SF2 (SRSF1 Human)

Tags

GST

Expression

Bacterial

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pFlag-CMV2-Brd4 D1346N

Plasmid

#21940

Depositor

Eric Verdin

Article

Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 1209-1362 only, D1346N

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only
pLenti-CMV-Blast-DNFGFR1-HA

Plasmid

#130887

Purpose

Expresses dominant negative mutant mouse FGFR1

Depositor

Gian-Paolo Dotto

Article

Bordignon et al Cell Rep. 2019 Aug 27;28(9):2358-2372.e6

Insert

Fgfr1 (Fgfr1 Mouse)

Tags

HA-tag

Expression

Mammalian

Mutation

Dominant Negative receptor lacking cytosolic cata…

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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CIp10 - mScarlet-I IDT

Plasmid

#163120

Purpose

Expresses in Candida albicans

Depositor

Patrick Van Dijck

Article

Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

Insert

mScarlet-I IDT

Use

Candida albicans expression

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLIB-HIS-TEV-CKS1

Plasmid

#177013

Purpose

Generate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CKS1 (CKS1B Synthetic, Human)

Tags

Polyhistidine-TEV

Expression

Insect

Mutation

codon-optimised for insect cell expression

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pSLI-N-sandwich-loxP(K13)

Plasmid

#85793

Purpose

P. falciparum integration plasmid for N-terminal gene fusions (double 2xFKBP) and placing loxP sites using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

Kelch13

Use

Cre/Lox; P. falciparum genomic integration

Tags

double 2xFKBP, GFP

Promoter

none

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pJAT13araE

Plasmid

#18987

Depositor

Jay Keasling

Article

Khlebnikov et al Microbiology. 2001 Dec . 147(Pt 12):3241

Insert

araE (araE E.coli)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
W45A mutant of H3K9me3 biosensor

Plasmid

#120808

Purpose

FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability

Depositor

Yingxiao Wang

Article

Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

Insert

Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3

Expression

Mammalian

Mutation

Tryptophan 45 is mutated to Alanine 45 on HP1 dom…

Promoter

CMV

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX cycT2b (P#1730)

Plasmid

#14603

Depositor

Matija Peterlin

Article

Kurosu et al Gene. 2004 Dec 8. 343(1):173-9.

Insert

cycT2 (CCNT2 Human)

Tags

GST

Expression

Bacterial

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only