We narrowed to 89,479 results for: MAL

Showing: 6681 - 6720 of 89479 results

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PE_CO

Plasmid

#190335

Purpose

Prime editor (PE2) with codon-optimized Reverse transcriptase for prime editing in mammalian cells

Depositor

Rasmus Bak

Article

Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22

Insert

PE2 with codon-optimized reverse transcriptase

Expression

Mammalian

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRRP12_K

Plasmid

#146766

Purpose

Mammalian Expression of HsRRP12

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsRRP12 (RRP12 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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YFP-mDia1FH2deltaN

Plasmid

#25419

Depositor

Arthur Alberts

Article

Wallar et al Exp Cell Res. 2007 Feb 1. 313(3):560-71.

Insert

diaph1 (Diaph1 Mouse)

Tags

YFP A206K

Expression

Mammalian

Mutation

mDia1 FH2∆N2 sequence (encodes amino acids 771-11…

Available Since

July 20, 2010

Availability

Academic Institutions and Nonprofits only
pEF HaloTag-SYB2

Plasmid

#197282

Purpose

Encodes full-length Synaptobrevin 2 (SYB2) with mutations Q76V and F77W to confer resistance to tetanus cleavage.

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYB2 and HaloTag (Vamp2 Rat)

Expression

Mammalian

Mutation

Q76V and F77W

Promoter

EF1alpha

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSIN REP5-GFP-GluR2 (Q)

Plasmid

#24003

Depositor

Robert Malinow

Article

Kessels et al Nat Neurosci. 2009 Jul . 12(7):888-96.

Insert

GluR2 (Gria2 Rat)

Use

Sindbis virus production

Tags

GFP

Mutation

R607Q

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mNFIL-3

Plasmid

#34572

Depositor

Peter Murray

Article

Smith et al J Biol Chem. 2011 Jul 1;286(26):23582-90

Insert

NFIL-3 (Nfil3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only
pTEV5_ RpMatB_1

Plasmid

#78205

Purpose

Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor

Depositor

Martin Webb

Article

Vancraenenbroeck et al ACS Chem Biol. 2015 Nov 20;10(11):2650-7

Insert

matB (His6 C106A R286C Q457C K488A)

Tags

His6

Expression

Bacterial

Mutation

C106A R286C Q457C K488A

Promoter

T7

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only
pLenti CMV/TO V5-LUC Puro (w549-1)

Plasmid

#19785

Purpose

3rd gen lentiviral V5-Luciferase expression vector, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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MSP1594

Plasmid

#65775

Purpose

Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS

Use

CRISPR

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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PB-CT3G-CERP2

Plasmid

#175499

Purpose

All-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G-mCMV

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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LentiGuidPuro-hTP73_sgRNA-1

Plasmid

#88855

Purpose

CRISPR KO of Trp73

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp73

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pAG223 PRKD1 3'UTR mut

Plasmid

#12042

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

PRKD1 3'UTR mut (PRKD1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
pTRE2-Bla(HA–RILP)

Plasmid

#102425

Purpose

HA tag fused to the N-terminus of RILP for the expression in mammalian cells.

Depositor

Steven Weinman

Article

Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

Rab interacting lysosomal protein (RILP Human)

Tags

HA

Expression

Mammalian

Promoter

Tet-responsive

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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PC17-605-9-1_BRD4-BD2-VPR

Plasmid

#200436

Purpose

Expresses the VPR transactivation domain linked to the BRD4(BD2) target protein

Depositor

Xue Gao

Article

Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1

Insert

BRD4(BD2)-VPR

Tags

BRD4(BD2), VPR

Expression

Mammalian

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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PC15-421-14-1_TRIM24-BD-VPR

Plasmid

#200435

Purpose

Expresses the VPR transactivation domain linked to the TRIM24(BD) target protein

Depositor

Xue Gao

Article

Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1

Insert

TRIM24(BD)-VPR

Tags

TRIM24(BD), VPR

Expression

Mammalian

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-DDK

Plasmid

#137764

Purpose

Control plasmid for immunoprecipitation of DDK/FLAG-tagged proteins. Also a good control for transfection efficiency.

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GFP

Tags

DDK

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pGinB-8X(GGS)-dCas9-FLAG-NLS

Plasmid

#81205

Purpose

CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

pGinB-8X(GGS)-dCas9-FLAG-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pBacT7-S1T3D

Plasmid

#33282

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

S1 (S1 Orthoreovirus T3D)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P33

Plasmid

#120018

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with FLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

FLAG

Use

Pcr template

Tags

FLAG

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z34

Plasmid

#120062

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xFLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xFLAG

Use

Pcr template

Tags

3xFLAG

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEJS2156_Nme2-ABE8e-nt

Plasmid

#201503

Purpose

Mammalian expression of N-terminally fused Nme2-ABE8e-nt

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-nt

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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Jnk3a1

Plasmid

#29577

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

Jnk3a1 (MAPK10 Human)

Tags

HIS6 and TEV

Expression

Bacterial

Mutation

Contains residues 40-402

Available Since

July 16, 2011

Availability

Academic Institutions and Nonprofits only
pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP

Plasmid

#185678

Purpose

Low-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-HaloTag-KKMP

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z22

Plasmid

#120065

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with SNAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

SNAP

Use

Pcr template

Tags

SNAP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pU6T7G

Plasmid

#71463

Purpose

Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (1 G at initiation)

Depositor

Willie Mark

Article

Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

human U6/phage T7

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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XMRV vp62

Plasmid

#26189

Depositor

Joseph DeRisi

Article

Urisman et al PLoS Pathog. 2006 Mar . 2(3):e25.

Insert

XMRV VP62 (gag Human)

Expression

Mammalian

Available Since

Nov. 16, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-myc-Sema4D

Plasmid

#51599

Purpose

expresses mouse Sema4D with N-terminal myc tag

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4D (Sema4d Mouse)

Tags

myc tag

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
EB3-mTurquoise2

Plasmid

#98825

Purpose

In vivo visualization of microtuble plus ends (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

EB3

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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J135F_BattB_Vif(T27X4)-IRES-SpyTag-sfmCherry2(11)-PuroR

Plasmid

#200637

Purpose

Recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

Vif

Expression

Mammalian

Mutation

T27stop

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pVH231-Tier1-PmPGK1-mTagBFP2

Plasmid

#169520

Purpose

Tier-1 vector encoding PmPGK1-driven mTagBFP2 expression (PmPGK1-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven monomeric Tag blue fluorescent protein 2

Expression

Mammalian

Promoter

PPGK

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z26

Plasmid

#120069

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mAID-Clover

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mAID-Clover

Use

Pcr template

Tags

mAID-Clover

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
1/8-NORAD-(A)4x

Plasmid

#199209

Purpose

1/8 truncation of NORAD mRNA tagged with 4 copies of Riboglow (variant A)

Depositor

Esther Braselmann

Article

Sarfraz et al Nat Commun. 2023 Feb 16;14(1):867. doi:

Insert

1/8-NORAD

Tags

Riboglow RNA tag

Expression

Mammalian

Mutation

4 copies of Riboglow tag after stop codon

Promoter

CMV

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiGuidPuro-hTP63_sgRNA-1

Plasmid

#88854

Purpose

CRISPR KO of Trp63

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp63

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
C1-Lyn-mCitrine-EEVEE-LifeAct

Plasmid

#155226

Purpose

Alternate MPAct using Lyn/LifeAct instead of CaaX/F-tractin

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lyn11 fused to mCitrine C-term tagged with a codon optimized EEVEE and LifeAct

Tags

mCitrine

Expression

Mammalian

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P27

Plasmid

#120038

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 1xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

1xHA

Use

Pcr template

Tags

1xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-P12

Plasmid

#120023

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet

Use

Pcr template

Tags

mScarlet

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PylT(UAG) PylS mCherry-P2A-eGFP(150TAG)

Plasmid

#174526

Purpose

Dual-fluorescence reporter for quantifying PylT(UAG) decoding efficiency

Depositor

Yu-Hsuan Tsai

Article

Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

Insert

PylS

Tags

Flag

Expression

Mammalian

Promoter

EF-1α

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
NAX_CAGGS_CAS9_m4_Blasto

Plasmid

#167864

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-HA-hAtg13

Plasmid

#22874

Depositor

Noboru Mizushima

Article

Hosokawa et al Autophagy. 2009 Oct . 5(7):973-9.

Insert

autophagy-related genes 13 (ATG13 Human)

Tags

HA

Expression

Mammalian

Available Since

March 25, 2010

Availability

Academic Institutions and Nonprofits only
1/2-NORAD-(A)4x

Plasmid

#199208

Purpose

1/2 truncation of NORAD mRNA tagged with 4 copies of Riboglow (variant A)

Depositor

Esther Braselmann

Article

Sarfraz et al Nat Commun. 2023 Feb 16;14(1):867. doi:

Insert

1/2-NORAD

Tags

Riboglow RNA tag

Expression

Mammalian

Mutation

4 copies of Riboglow tag after stop codon

Promoter

CMV

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 89,479 results for: MAL