We narrowed to 5,896 results for: dna plasmids

1. FH-TET1-pEF

   Plasmid

   #49792

   Purpose

   This plasmid expresses the full-length human TET1 gene with a Flag and HA tag.

   Depositor

   Anjana Rao

   Article

   Tahiliani et al Science. 2009 May 15;324(5929):930-5. Ep

   Insert

   Tet methylcytosine dioxygenase 1 (TET1 Human)
   

   Tags

   Flag and HA

   Expression

   Mammalian

   Promoter

   EF-1alpha

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDriver

   Plasmid

   #165149

   Purpose

   Driver plasmid for PROBER encoding SV40 Large T-antigen

   Depositor

   Paul Khavari

   Article

   Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

   Insert

   SV40 Large T-antigen
   

   Expression

   Mammalian

   Available Since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. hACE2

   Plasmid

   #1786

   Purpose

   Mammalian expression of human ACE2

   Depositor

   Hyeryun Choe

   Article

   Li et al Nature. 2003 Nov 27. 426(6965):450-4.

   Has Service

   Cloning Grade DNA

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Tags

   C9 (GTETSQVAPA)

   Expression

   Mammalian

   Available Since

   Feb. 14, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. SuExp His-Myc-hPLD3

   Plasmid

   #173851

   Purpose

   Protein expression plasmid for recombinant His-Myc tag human PLD3

   Depositor

   David Nemazee

   Article

   Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

   Insert

   PLD3 (PLD3 Human)
   

   Tags

   6xHis, Myc, Factor X cleavable

   Expression

   Mammalian

   Mutation

   intracellular and transmembrane domain truncated,…

   Promoter

   CMV

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. SuExp His-Myc-hPLD4

   Plasmid

   #173852

   Purpose

   Protein expression plasmid for recombinant His-Myc tag human PLD4

   Depositor

   David Nemazee

   Article

   Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

   Insert

   PLD4 (PLD4 Human)
   

   Tags

   6xHis, Myc, Factor X cleavable

   Expression

   Mammalian

   Mutation

   intracellular and transmembrane domain truncated,…

   Promoter

   CMV

   Available Since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. P022-mPB-TRE-PEmax-T2A-mCherry-PGK-rttA-Puro

   Plasmid

   #239974

   Purpose

   PiggyBac plasmid for inducible PEmax expression and Puromycin selection

   Depositor

   Jay Shendure

   Article

   Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   June 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-RIGI-Apex- T2A-EGFP

   Plasmid

   #167288

   Purpose

   Lentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   DDX58 (RIGI Human)
   

   Use

   Lentiviral

   Tags

   APEX2 and EGFP

   Expression

   Mammalian

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. NSUN2

   Plasmid

   #188059

   Purpose

   Bacterial expression plasmid for human NSUN2; for activity testing

   Depositor

   Tanja Schirmeister

   Article

   Schwickert et al J Med Chem. 2022 Jul 18. doi: 10.1021/ac

   Insert

   NSUN2 (NSUN2 Human)
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CGBE1 (pRZ3885)

   Plasmid

   #140252

   Purpose

   CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   eUNG-BE4max(R33A)-ΔUGI
   

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, D10A in Cas9

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-SpRY-NLS-6xHis (gbr2101)

    Plasmid

    #181743

    Purpose

    bacterial expression plasmid for SpRY with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    bacterial codon optimized SpRY
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS(SV40)-6xHis and MKIEE MBP tag

    Expression

    Bacterial

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    T7

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSprayerHE

    Plasmid

    #165150

    Purpose

    Sprayer plasmid for PROBER encoding BASU-GAL4 fusion (High Expression)

    Depositor

    Paul Khavari

    Article

    Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

    Insert

    BASU-GAL4
    

    Expression

    Mammalian

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBait

    Plasmid

    #165146

    Purpose

    Bait plasmid for PROBER (empty backbone encoding replaceable ccdB cassette)

    Depositor

    Paul Khavari

    Article

    Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSprayer

    Plasmid

    #165148

    Purpose

    Sprayer plasmid for PROBER encoding BASU-GAL4 fusion

    Depositor

    Paul Khavari

    Article

    Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

    Insert

    BASU-GAL4
    

    Expression

    Mammalian

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBait-YY1

    Plasmid

    #165147

    Purpose

    Bait plasmid for PROBER with YY1-binding site (positive control)

    Depositor

    Paul Khavari

    Article

    Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

    Insert

    3X YY1 motif
    

    Use

    Unspecified

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-3-FT-ENGRAM

    Plasmid

    #179158

    Purpose

    piggyBac plasmid for 3'-FT ENGRAM recorder

    Depositor

    Jay Shendure

    Article

    Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

    Insert

    Csy4
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    minP

    Available Since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti

    Plasmid

    #135955

    Purpose

    Neuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3b

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    Prime editor v2 (PE2) N-terminal
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. hSynapsin-PE2-Cterminal-lenti

    Plasmid

    #135956

    Purpose

    Neuronal expression of C-terminal intein-split prime editor v2 (PE2). This plasmid is used by PE2, PE3, and PE3b

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    Prime editor v2 (PE2) C-terminal
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    MMLV RT(D200N, T306K, W313F, T330P, L603W)

    Promoter

    hSynapsin

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Bxb1-GT_AIO-TetOn_mNgn2 _Down-Tandem

    Plasmid

    #229795

    Purpose

    Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of mouse neurogenin 2

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mNgn2; rtTA-T2A-mTagBFP2 (Neurog2 Mouse)
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    TRE and CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-PB-SRT-tdTomato

    Plasmid

    #154889

    Purpose

    piggyBac self-reporting transposon with tdTomato marker in AAV backbone

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    piggyBac SRT
    

    Use

    Adenoviral

    Expression

    Mammalian

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. SunTag-FWAgRNA-4-22aa-TET1cd

    Plasmid

    #106435

    Purpose

    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

    Depositor

    Steven Jacobsen

    Article

    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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