We narrowed to 4,886 results for: phy

1. pmApple-GNG2

   Plasmid

   #133857

   Purpose

   Mammalian expression of GNG2 N-terminally tagged with mApple

   Depositor

   Dave Piston

   Article

   Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

   Insert

   GNG2 (GNG2 Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-human NMyc-humanER-IRES-mCherry

   Plasmid

   #73571

   Purpose

   Mammalian retroviral expression of NMyc-ER

   Depositor

   Martine Roussel

   Article

   Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

   Insert

   NMyc (MYCN Human)
   

   Use

   Retroviral

   Tags

   Human ER and IRES-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pMDC123SB-AtTAS1c-B/c

   Plasmid

   #51772

   Purpose

   Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor

   Depositor

   James Carrington

   Article

   Carbonell et al Plant Physiol. 2014 Mar 19.

   Insert

   AtTAS1c-B/c
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   A. thaliana TAS1c precursor sequence including a …

   Promoter

   Available since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHMT.wt (p53)

   Plasmid

   #12139

   Depositor

   James Sherley

   Article

   Liu et al J Cell Physiol. 1998 Nov . 177(2):364-76

   Insert

   p53 (Trp53 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pLentiCRISPR-v1-sgHK2_2

   Plasmid

   #163461

   Purpose

   lentiviral vector expressing Cas9 and an sgRNA targeting HK2

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   sgRNA 2 targeting HK2 (HK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEIAV-DIO-TLoop-CHR2YFP

   Plasmid

   #66798

   Purpose

   expression of channelrhodopsin-2-YFP, a light-gated cation channel that can depolarize cells via sodium influx when blue light changes its conformation, using a DIO-based TLoop EIAV vector

   Depositor

   Edward Callaway

   Article

   Cetin et al J Neurophysiol. 2014 May;111(10):2150-9.

   Insert

   Floxed Chr2-YFP
   

   Use

   Lentiviral

   Tags

   citrine

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   CMVTetO7 Promoter

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHLmMBP-2

   Plasmid

   #72344

   Purpose

   Mammalian expression of secreted N-terminally 6His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

   Depositor

   Luca Jovine

   Article

   Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

   Type

   Empty backbone

   Use

   Tags

   6His-tag, HA-tag, Strep-tag II, and mMBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer + chicken beta-actin promoter

   Available since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-51b(+)-Caprola_09

   Plasmid

   #194670

   Purpose

   Protein production of the split HaloTag based calcium recorder Caprola_09 in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_09
   

   Use

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AA299

   Plasmid

   #216029

   Purpose

   Fragmid fragment: (Cas protein) NNGG PAM

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Cas9_v1.1 [Slug]
   

   Use

   CRISPR; Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. AA180

    Plasmid

    #216023

    Purpose

    Fragmid fragment: (Cas protein) NGRR(T) PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9_v1.1 [Sa]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AA301

    Plasmid

    #216030

    Purpose

    Fragmid fragment: (Cas protein) NNGG PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9_v1.1 [Sauri]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-D2s-L-V1

    Plasmid

    #19967

    Depositor

    Jonathan Javitch

    Article

    Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

    Insert

    D2 short variant-N terminal Venus (DRD2 Human, Aequorea Victoria)
    

    Use

    Tags

    Flag and N terminal Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

13. Actin binding domain of Ezrin

    Plasmid

    #62381

    Purpose

    Expresses the actin binding domain of ezrin. Amino acids 552-586.

    Depositor

    Guillaume Charras

    Article

    Fritzsche et al Biophys J. 2014 Jan 21;106(2):343-53. do

    Insert

    Actin binding domain of ezrin (EZR Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains aa's 552-586

    Promoter

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-ABE-NT-sgRNA

    Plasmid

    #112734

    Purpose

    AAV inverted terminal repeat based vector plasmid encoding E. coli TadA, the N-terminal half of nCas9 and sgRNA

    Depositor

    Jin-Soo Kim

    Article

    Ryu et al Nat Biotechnol. 2018 Jul;36(6):536-539.

    Insert

    ABE-NT-sgRNA
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Sa-dCas9-NLS-3xFLAG/pcDNA3.1

    Plasmid

    #98041

    Purpose

    Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Insert

    Sa-dCas9-NLS-3xFLAG
    

    Use

    CRISPR

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    D10A + H557A

    Promoter

    CMV

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDest-636

    Plasmid

    #159574

    Purpose

    Gateway Destination vector for protein expression in baculovirus

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Type

    Empty backbone

    Use

    Tags

    His6-MBP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-AtTAS1c-B/c

    Plasmid

    #51774

    Purpose

    Gateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor

    Depositor

    James Carrington

    Article

    Carbonell et al Plant Physiol. 2014 Mar 19.

    Insert

    AtTAS1c-B/c
    

    Use

    Gateway-compatible entry clone

    Tags

    Expression

    Mutation

    A. thaliana TAS1c precursor sequence including a …

    Promoter

    Available since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. LZF1826 pAAV_hSyn-DiO-SomArchon-EGFP-P2A-stGtACR2

    Plasmid

    #135412

    Purpose

    simultaneous optical recording and manipulation of electrical activity in neurons

    Depositor

    Adam Cohen

    Article

    Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

    Insert

    SomArchon-EGFP-P2A-stGtACR2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. attB-mCh-Poly(A)

    Plasmid

    #65525

    Purpose

    Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attB-mCherry
    

    Use

    Mouse Targeting; Bxb1

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-D-TOPO-kcnn3

    Plasmid

    #164959

    Purpose

    Zebrafish kcnn3 gene CDS

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

    Insert

    kcnn3 (kcnn3 Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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