We narrowed to 4,243 results for: Pet2

1. pJBL723

   Plasmid

   #140393

   Purpose

   Expresses HucR protein under the control of T7-lacO promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-lacO-HucR-T
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pETNb-cALFA

   Plasmid

   #159989

   Purpose

   For the protein expression with ALFA tag at C-terminus in E. coli

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Use

   Tags

   HRV3C protease cleavage site-ALFA tag-8histidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. HECTD1

   Plasmid

   #32860

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   HECTD1 (HECTD1 Human)
   

   Use

   Tags

   His (with TEV cleavage)

   Expression

   Bacterial

   Mutation

   amino acids 1879-1966 only

   Promoter

   Available since

   Jan. 6, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pJBL713

   Plasmid

   #140383

   Purpose

   Expresses CtcS protein under the control of T7-lacO promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-lacO-CtcS-T
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FnR9-10

   Plasmid

   #182567

   Purpose

   Human fibronectin fragment, type-III repeats 9-10, R1379A mutant

   Depositor

   Timothy Springer

   Article

   Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

   Insert

   Human fibronectin fragment, type-III repeats 9-10
   

   Use

   Tags

   His and TEV cleavage site after His tag

   Expression

   Bacterial

   Mutation

   R1379A

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Human USP30 (64-502, construct 8, MG-26-21)

   Plasmid

   #110745

   Purpose

   Bacterial expression of human USP30 (construct 8)

   Depositor

   David Komander

   Article

   Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

   Insert

   USP30 (USP30 Human)
   

   Use

   Tags

   N-His6-3C-GST-3C

   Expression

   Bacterial

   Mutation

   F348D, M350D, I353E + insertion deletion: 64-357;…

   Promoter

   T7

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pETZZtevPP7CP(C68A,C73A)

   Plasmid

   #27548

   Depositor

   Kathleen Collins

   Article

   Hogg et al RNA. 2007 Jun . 13(6):868-80.

   Insert

   Pseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)
   

   Use

   Tags

   TEV protease cleavage site, six histidine tag, an…

   Expression

   Bacterial

   Mutation

   changed two cysteine to alanine (C68A,C73A) to in…

   Promoter

   Available since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PT7lacO-His6-AlaRS

   Plasmid

   #224362

   Purpose

   Express AlaRS protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   AlaRS (alaS E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SurA

   Plasmid

   #213138

   Purpose

   Expresses chaperone SurA

   Depositor

   Sebastian Hiller

   Article

   Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

   Insert

   SurA (21-428) (surA E.coli)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAG247 His-iFAST

    Plasmid

    #130808

    Purpose

    Expresses His-tagged iFAST in bacteria

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

    Insert

    iFAST
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SARS-CoV-2 Mpro protease (M165I mutant - clinical mutant); aliases: 3CLpro, 3C-like

    Plasmid

    #204778

    Purpose

    Bacterial expression of Sars Cov 2 MPRO M165I mutant

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Sars Cov 2 MPRO (ORF1ab Sars Cov 2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    M165I

    Promoter

    Available since

    Dec. 1, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. +36GFP-LPETG

    Plasmid

    #71754

    Purpose

    +36GFP-LPETG for expression in E.Coli

    Depositor

    David Liu

    Article

    Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

    Insert

    (GGS)9_(pos36GFP)_6XHis_LPETG
    

    Use

    Tags

    6XHis and Sortase (LPETG)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET16_Caspase-4_p10

    Plasmid

    #214308

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pC28-LIC-sfGFP-His

    Plasmid

    #165479

    Purpose

    pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

    Depositor

    Renato Bruni

    Article

    Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

    Type

    Empty backbone

    Use

    Tags

    TEV-GFP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Human RNF144A (16-228)

    Plasmid

    #110752

    Purpose

    Bacterial Expression of RNF144A RBR domain

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2017 Oct 5;68(1):233-246.e5. d

    Insert

    RNF144A (RNF144A Human)
    

    Use

    Tags

    N-His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 6BY9 (EHMT1)

    Plasmid

    #119956

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    EHMT1 (EHMT1 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pGBMCS-SortA

    Plasmid

    #21931

    Depositor

    Fuyuhiko Inagaki

    Article

    Kobashigawa et al J Biomol NMR. 2009 Mar . 43(3):145-50.

    Insert

    sortase (srtA Staphylococcus aureus (strain N315))
    

    Use

    Tags

    GB1 and His-tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59

    Promoter

    Available since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

18. tUI-HA

    Plasmid

    #122662

    Purpose

    Express tUI-HA protein in E. coli

    Depositor

    Robert Cohen

    Article

    Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Insert

    tUI-HA
    

    Use

    Tags

    HA-tag and His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 17, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. JMC044-B12

    Plasmid

    #58752

    Purpose

    bacterial expression of PDB 4NW3

    Depositor

    Cheryl Arrowsmith

    Insert

    PDB:4NW3 (KMT2A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Promoter

    T7

    Available since

    Aug. 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

20. KRASB

    Plasmid

    #25153

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    KRASb:M1-K169 (KRAS Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Q61H

    Promoter

    Available since

    Aug. 6, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits