We narrowed to 20,271 results for: ACE;

1. NLS-GI-VP64-IRES-NLS-GAL4DBD-M13-SmBiT-FKF1

   Plasmid

   #183400

   Purpose

   Dual expression of GI-VP64 and GAL4DBD-M13-SmBiT-FKF1 in the nucleus

   Depositor

   Alice Ting

   Article

   Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

   Insert

   GI-VP64-IRES-GAL4DBD-M13-SmBiT-FKF1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICSL70008

   Plasmid

   #50336

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   pFAST-R selection cassette (monomeric tagRFP from E. quadricolor fused to the coding sequence of OLE1 (A. thaliana)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. NLS-GI-VP16-IRES-NLS-GAL4DBD-FKBP-SMBiT-FKF1

   Plasmid

   #183402

   Purpose

   Dual expression of GI-VP16 and GAL4DBD-FKBP-SmBiT-FKF1 in the nucleus

   Depositor

   Alice Ting

   Article

   Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

   Insert

   GI-VP16-IRES-GAL4DBD-FKBP-SMBiT-FKF1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Myr.PKCzeta.FLAG

   Plasmid

   #10802

   Depositor

   Alex Toker

   Article

   Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

   Insert

   PKC zeta (Prkcz Rat)
   

   Use

   Tags

   FLAG and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 26, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pMRMTK-clo6

   Plasmid

   #203957

   Purpose

   Cloning plasmid containing the kanamycin resistance gene insert

   Depositor

   Nathan Crook

   Article

   van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

   Insert

   Kanamycin Resistance Gene
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUb-HisUb-Ubc9-ChE1 v4.5

   Plasmid

   #185756

   Purpose

   Bacterial expression of His-Ub, Ubc9 and ChE1 v4.5

   Depositor

   Jeffrey Bode

   Article

   Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

   Inserts

   His-Ub
   Ubc9
   ChE1 v4.5
   

   Use

   Tags

   His6

   Expression

   Mutation

   K14R

   Promoter

   T7

   Available Since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. LV-TRE-VP16 mouse MyoD-T2A-dsRedExpress2

   Plasmid

   #60626

   Purpose

   Expresses VP16 mouse MyoD and dsRedExpress2 in response to doxycycline

   Depositor

   Charles Gersbach

   Article

   Kabadi et al ACS Synth Biol. 2014 Dec 10.

   Insert

   VP16 mouse MyoD
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pICSL70004

   Plasmid

   #50334

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   kanamycin/neomycin/paromomycin resistance cassette (Nos promoter (A. tumefaciens) + NptII (E.coli) + Ocs terminator (A. tumefaci
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pProm1_BCD1-GFP

   Plasmid

   #80649

   Purpose

   Promoter1 with BCD1-gfp

   Depositor

   Nathan Hillson

   Article

   Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

   Insert

   GFP
   

   Use

   P15a origin, constitutive promoter, kanr

   Tags

   Expression

   Mutation

   NONE

   Promoter

   constitutive promoter

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWT35S-Cerulean-SKL

    Plasmid

    #194048

    Purpose

    Transient expression of Cerulean-SKL in plant cell (Peroxisome)

    Depositor

    Yutaka Kodama

    Article

    Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

    Insert

    Cerulean
    

    Use

    Tags

    Ser-Lsy-Leu

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO_LAMP1_STf

    Plasmid

    #182006

    Purpose

    CMV overexpression of SNAP-tag fast in the lysosome of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    LAMP1_STf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pgRNA-non-target

    Plasmid

    #129465

    Purpose

    Derived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Non-target/random gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 (SpeI)

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMC-XI2

    Plasmid

    #176156

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-2

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. DVK_AF

    Plasmid

    #66068

    Purpose

    MoClo Destination Vector: (based on pSB1K3) [A:LacZa:F]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pUPD2+E-Luc

    Plasmid

    #170873

    Purpose

    Phytobrick - E-Luc Luciferase CDS

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    c.375C>T to remove BbsI site

    Promoter

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS423-TyrSgH

    Plasmid

    #163973

    Purpose

    Helper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoter

    Depositor

    Jiazhang Lian

    Article

    Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCas13 Twin-SUMO

    Plasmid

    #199753

    Purpose

    Expresses codon optimized miniature mCas13 for protein expression in E.coli

    Depositor

    Magdy Mahfouz

    Article

    Mahas et al ACS Synth Biol. 2021 Oct 15;10(10):2541-

    Insert

    mCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    LacI

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. qTAG-C-miniTurbo-Zeo

    Plasmid

    #207736

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. qTAG-N-Zeo-miniTurbo

    Plasmid

    #207703

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-miniTurbo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pRPL28(K55M) SpHIS5 (pNTI666)

    Plasmid

    #115436

    Purpose

    Expresses K55M RPL28 from HIS3 yeast plasmid

    Depositor

    Nicholas Ingolia

    Article

    McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

    Inserts

    RPL28 (RPL28 Budding Yeast)
    HIS5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    K55M; MfeI site in intron

    Promoter

    Ashbya gospii TEF1 and endogenous

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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