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We narrowed to 2,300 results for: tub

Showing: 481 - 510 of 2300 results

  5. pBS/U6/RNAi gamma-tubulin shRNA mouse

    Plasmid
    #70666
    Purpose
    Reduced the expression of gamma-tubulin in murine cells
    Depositor
    Maria Alvarado-Kristensson
    Article
    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1
    Insert
    5´-GGCTCATGATGGCCAACCACCAAGCTTGGTGGTTGGCCATCATGAGCCCTTTTTC-3´ 5´-CCGAGTACTACCGGTTGGTGGTTCGAACCACCAACCGGTAGTACTCGGGAAAAAGTTAA-3´ (Tubg1, Tubg2 Mouse)
    Use
    U6 promoter
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    70666-plasmid-map-sequence-id-133021

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  6. pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC

    Plasmid
    #234370
    Purpose
    T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs
    Depositor
    Michael Smanski
    Article
    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70
    Inserts
    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-13XGP41
    gRNA 1-1 and gRNA 1-2
    Use
    Synthetic Biology
    Tags
    GB1, GP41, and sfGFP
    Expression
    Plant
    Mutation
    Promoter
    Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234370-plasmid-map-sequence-id-469430

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  7. pMT1-13X-NOG(AtUBI10)-MTAP-LUC

    Plasmid
    #234371
    Purpose
    T-DNA vector for expression of the the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs, no gRNA included (NOG)
    Depositor
    Michael Smanski
    Article
    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70
    Inserts
    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-13XGP41
    Use
    Synthetic Biology
    Tags
    GB1, GP41, and sfGFP
    Expression
    Plant
    Mutation
    Promoter
    Arabidopsis Ubi10 and MTAP1 (synthetic)
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234371-plasmid-map-sequence-id-469425

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  8. pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC

    Plasmid
    #234372
    Purpose
    T-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs
    Depositor
    Michael Smanski
    Article
    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70
    Inserts
    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-24XGP41
    gRNA 1-1 and gRNA 1-2
    Use
    Synthetic Biology
    Tags
    GB1, GP41, and sfGFP
    Expression
    Plant
    Mutation
    Promoter
    Arabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234372-plasmid-map-sequence-id-469423

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  10. L0I101- GG4-pGATA23-Bxb1-tUBQ10-GG6

    Plasmid
    #219712
    Purpose
    Level 0 pGATA23-Bxb1-tUBQ10 construct for assembly of two integrase constructs, GG4 and GG6 BsaI sites.
    Depositor
    Jennifer Nemhauser
    Insert
    GG4-pGATA23-Bxb1-tUBQ10-GG6
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219712-plasmid-map-sequence-id-435863

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  11. L0I90 - GG0-pSPCH-PhiC31-tUBQ1-GG4

    Plasmid
    #219709
    Purpose
    Level 0 pSPCH-PhiC31-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pSPCH:PhiC31:tUBQ1-GG4
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    219709-plasmid-map-sequence-id-435862

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  12. L0-T14 GG0-tUBQ1-NLS-NeonGreen-GG1

    Plasmid
    #219697
    Purpose
    Level 0 to construct history-dependent target
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-tUBQ1-NLS-NeonGreen-GG1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    219697-plasmid-map-sequence-id-435859

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  13. pCDNA5/FRT/TO–γ-tubulinWT-RNAi-resistant-GFP

    Plasmid
    #205603
    Purpose
    Tet-inducible expression of WT gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion
    Depositor
    Tarun Kapoor
    Article
    Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410
    Insert
    γ-tubulinWT-RNAi-resistant-GFP
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    G2228A, C2231T, C2237T, G2240A
    Promoter
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    205603-plasmid-map-sequence-id-405874

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  14. pCDNA5/FRT/TO–γ-tubulinΔGTP-RNAi-resistant-GFP

    Plasmid
    #205604
    Purpose
    Tet-inducible expression of N229A gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion
    Depositor
    Tarun Kapoor
    Article
    Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410
    Insert
    γ-tubulinΔGTP-RNAi-resistant-GFP
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    G2228A, C2231T, C2237T, G2240A, N229A
    Promoter
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    205604-plasmid-map-sequence-id-405873

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  21. pBac[3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP]

    Plasmid
    #86456
    Purpose
    Lox responder construct with floxed (same orientation; 2xloxP, 2xlox2272) mCherry (cy) body marker and H2Av::EGFP (nu) for Cre dependent cell clone marking; (Transformation plasmid; Black eye marker)
    Depositor
    Gregor Bucher
    Insert
    3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86456-plasmid-map-sequence-id-171156

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  22. L0I97 - GG0-pAHP6-PhiC31-NLS-tUBQ1-GG4

    Plasmid
    #219711
    Purpose
    Level 0 pAHP6-PhiC31-NLS-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.
    Depositor
    Jennifer Nemhauser
    Insert
    GG0-pAHP6:PhiC31-NLS:tUBQ1-GG4
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219711-plasmid-map-sequence-id-435738

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  23. pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NELC492A-mcherry-Tnos

    Plasmid
    #211844
    Purpose
    Plant transient expression cassette for the catalytically inactive E3-DART ligase containing a nuclear localization signal.
    Depositor
    Marcela Rojas-Pierce
    Article
    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.
    Insert
    LRR-NEL
    Use
    Synthetic Biology
    Tags
    3xNLS-HA and mCherry
    Expression
    Plant
    Mutation
    C492A
    Promoter
    UBIQUITIN10
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    211844-plasmid-map-sequence-id-416933

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  24. pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP

    Plasmid
    #176531
    Purpose
    PiggyBAC vector expressing a FOS promoter driven "KGV" synthetic transcription factor and a CMV promoter driven BFP marker.
    Depositor
    Jared Toettcher
    Article
    Ravindran et al Cell Syst. 2022 Feb 16;13(2):131-142.e13
    Inserts
    KGV transcription factor
    TagBFP
    Use
    Piggybac transposase integration vector
    Tags
    ErkKTR, Gal4 DNA binding domain, PEST destabiliza…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    176531-plasmid-map-sequence-id-375608

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  27. 6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)

    Plasmid
    #169168
    Purpose
    Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.
    Depositor
    Sascha Martens
    Article
    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017
    Insert
    MAP1LC3B (MAP1LC3B Human)
    Use
    Tags
    6X Histidine Tag, TEV cleavage site, mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac promoter
    Available since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169168-plasmid-map-sequence-id-332048

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  29. pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NEL-mcherry-Tnos

    Plasmid
    #211842
    Purpose
    Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase
    Depositor
    Marcela Rojas-Pierce
    Article
    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.
    Inserts
    LRR-NEL
    GAL4-VP16-GR
    Use
    Synthetic Biology
    Tags
    HA, Myc, and mCherry
    Expression
    Plant
    Mutation
    Promoter
    6xUAS-35Smini and pUBQ10
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    211842-plasmid-map-sequence-id-422272

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  30. pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NELC492A-mcherry-Tnos

    Plasmid
    #211845
    Purpose
    Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase, where the ligase is catalytically inactive.
    Depositor
    Marcela Rojas-Pierce
    Article
    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.
    Inserts
    LRR-NEL
    GAL4-VP16-GR
    Use
    Synthetic Biology
    Tags
    HA, Myc, and mCherry
    Expression
    Plant
    Mutation
    C492A
    Promoter
    6xUAS-35Smini and pUBQ10
    Available since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    211845-plasmid-map-sequence-id-426288

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Showing: 481 - 510 of 2300 results