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Plasmid#212144PurposePreparatory plasmid for operon integration into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
UseTagsExpressionBacterialMutationPromoterpJ23119Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_GFPsp_CamRcargo
Plasmid#212143PurposePreparatory plasmid for gene disruption into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
UseTagsExpressionBacterialMutationPromoterpJ23119Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-eGFP-P(11)4
Plasmid#185786PurposeEncodes enhanced green fluorescent protein linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInserteGFP-P(11)4
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
GGE261
Plasmid#120783PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Coding sequence of fluorescent protein in position 3 (G3_mTurquoise)DepositorInsertG3_mTurquoise
UseCloning/assembly plasmidTagsExpressionMutationPromoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYIC
Plasmid#18673DepositorTypeEmpty backboneUseTags3Myc-EYFP-HA-His6 and ECFP-HA-His6ExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
Dimer 42 AA
Plasmid#198198PurposeFRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 42AA linkerDepositorInsertmVenus-42AA-mTurquoise2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dimer 3 AA
Plasmid#198197PurposeFRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 3AA linkerDepositorInsertmVenus-3AA-mTurquoise2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-GFP
Plasmid#13770PurposeCre recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.DepositorInsertEGFP
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET30-R5-GFP
Plasmid#17838DepositorInsertpET30-GFP
UseSynthetic BiologyTagsHis, R5, and S-tagExpressionBacterialMutationPromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
ATeam1.03-nD/nA/pcDNA3
Plasmid#51958Purposemammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicatorDepositorInsertATeam1.03-nD/nA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSW039
Plasmid#140035PurposeKO of PCC 11901 psbA2 gene/expresses YFP under control of inducible cLac143 promoterDepositorInsertYellow fluorescent protein
UseTagsExpressionBacterialMutationPromotercLac143Available sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pST5552
Plasmid#36255PurposeMycobacteria replicating vector expressing eGFP.DepositorInserteGFP
UseSynthetic Biology; Mycobacteria replicating vectorTagsExpressionBacterialMutationPromoterModifiedHsp60Available sinceMay 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPAGFP-N1
Plasmid#11909DepositorHas ServiceCloning Grade DNAInsertpPAGFP-N1
UseTagsExpressionMammalianMutationThese plasmids are essentially the same as the Cl…PromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pPAGFP-C1
Plasmid#11910DepositorHas ServiceCloning Grade DNAInsertPAGFP
UseTagsExpressionMammalianMutationThese plasmids are essentially the same as the Cl…PromoterAvailable sinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-qmTQ2-cAMP
Plasmid#225250Purposemammalian expression of qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMPDepositorInsertqmTQ2-cAMP
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYFP
Plasmid#175592PurposeTet inducible, KAN Resistant, mVenus gene on a low copy backbone. Used as a negative control to the pRelA_YFP plasmid.DepositorInsertmVenus
UseTagsExpressionBacterialMutationAn improved monomeric YFP. See doi: 10.1021/bi051…PromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Zeo-BFP
Plasmid#214367PurposeExpression of the Blue Fluorescent ProteinDepositorInsertBFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-GFP
Plasmid#13772PurposeFlp recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Flp due to a transcriptional stop in between the FRT sites.DepositorInsertEGFP
UseFlp/frtTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMPMA3dPlac-null-GFPLVA
Plasmid#23338DepositorInsertGFP(LVA)
UseTagsGFP(LVA)ExpressionBacterialMutationPromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNCS YFP
Plasmid#91759PurposeBacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)DepositorInsertVenus YFP (A206K)
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-qmTQ2-ATP
Plasmid#225248Purposemammalian expression of qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imagingDepositorInsertqmTQ2-ATP
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSW036
Plasmid#140034PurposeKO of PCC 11901 acsA gene/expresses YFP under control of the cpt promoterDepositorInsertYellow fluorescent protein
UseTagsExpressionBacterialMutationPromotercptAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
p426MET25_sfpHluorin (MRV55)
Plasmid#115697Purposesuperfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)DepositorInsertsfpHluorin
UseTagsExpressionYeastMutationfor introduced mutations see publicationPromoterMET25Available sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLOVE-GFP
Plasmid#15949DepositorInsertGreen Fluorescent Protein
UseLentiviralTagsV5ExpressionMammalianMutationThe recombination reaction required to clone &quo…PromoterAvailable sinceNov. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET21a.Tat(48-57)-EGFP
Plasmid#165040PurposeExpression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)DepositorInsertEGFP
UseTags6XHis and Tat(48-57)ExpressionBacterialMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSfinx-mYFP
Plasmid#216206PurposeA simplified disease resistance assay using YFP-expressing Potato Virus X in N. benthamianaDepositorInsertmonomeric yellow fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E tdTomato
Plasmid#135210PurposeRed fluorescent tagDepositorInserttdTomato
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3E p2a-tomato
Plasmid#135211PurposeRed fluorescent tagDepositorInserttdTomato
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ateam1.03-nD/nA/pENTR1A
Plasmid#51959PurposeGateway entry vector containing ATeam1.03-nD/nA, a FRET-based ATP indicatorDepositorInsertATeam1.03-nD/nA
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
Ateam1.03-nD/nA/pRSETB
Plasmid#51960Purposebacterial expression of ATeam1.03-nD/nA, a FRET-based ATP indicatorDepositorInsertATeam1.03-nD/nA
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGFP
Plasmid#64904Purpose"Fluorescent reporter containing no disulfide bonds/Selenocystamine folding"DepositorInsertGreen fluorescent protein
UsePsba chlamydomonas reinhardtii vectorTagsExpressionMutationPromoterpsbAAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUNIV-EGFP
Plasmid#24706DepositorInsertEGFP (eGFP Aequoria victoria)
UseTagsExpressionBacterial, Mammalian, and Y…MutationPromoterAvailable sinceDec. 6, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTCW012
Plasmid#83527PurposeCloning destination vector for phermone mediated expression from the FUS1J2 promoter and CYC1 terminatorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn 2xLyn-ERex-mKate2-bPAC(F198Y)
Plasmid#219654PurposeNeuronal expression of PACmn with mKate2, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity.DepositorInsert2xLyn-ERex-mKate2-bPAC(F198Y)
UseAAVTagsER exit ( FCYENE) and Lyn11 (GCIKSKGKDS)ExpressionMammalianMutationPromoterSynAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR
Plasmid#47643Purposewt esaR gene under control of Plac in pACYC184DepositorInsertPlac-esaR-terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
Launchpad (AVA2590)
Plasmid#85442PurposeLentiviral FRT-flanked EF1alfa-GFP PGK-PuromycinR cassetteDepositorInsertFRT-flanked EF1alfa-GFP PGK-Puromycin cassette
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOz-humBirA-GFP
Plasmid#133283PurposeExpression of BirA-GFP fusions in mammalian cellsDepositorInserteGFP
UseRetroviralTagsHumanized wild type biotin ligase BirAExpressionMammalianMutationPromoterMoMuLVAvailable sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFP
Plasmid#175593PurposeLac inducible, AMP Resistant, cerulean gene on a low copy backbone. Used as a negative control to the pMesh1_CFP plasmid.DepositorInsertCFP (cerulean)
UseTagsExpressionMutationAn improved monomeric CFP. See doi: 10.1038/nbt945PromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFPL-Ch
Plasmid#61647PurposeGateway vector for generating cyan fluorescent protein fusions in fungiDepositorInsertenhanced cyan fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFPL-Gh
Plasmid#61648PurposeGateway vector for generating green fluorescent protein fusions in fungiDepositorInsertenhanced green fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only