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We narrowed to 14,928 results for: NOL

Showing: 801 - 850 of 14928 results

  1. pFLAG_attP

    Plasmid
    #110095
    Purpose
    Vector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110095-plasmid-map-sequence-id-317064

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  2. pMA_Int

    Plasmid
    #110096
    Purpose
    E. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Insert
    mycophage integrase
    Use
    Required for transformation of attb integrating v…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110096-plasmid-map-sequence-id-211299

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  3. pACEC3GH (Apr)

    Plasmid
    #110085
    Purpose
    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    3C cleavage site - GFP - His10 Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110085-plasmid-map-sequence-id-211188

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  4. pExpreS2-1-C3-PA

    Plasmid
    #175446
    Purpose
    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform
    Depositor
    Wyatt Yue
    Article
    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8
    Type
    Empty backbone
    Use
    Tags
    3C-Protein A and BiP Secretion Peptide
    Expression
    Insect
    Mutation
    Promoter
    Fused Actin-HSP70
    Available since
    March 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    175446-plasmid-map-sequence-id-358138

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  7. pET28a Gx-NL2

    Plasmid
    #169107
    Purpose
    Expresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.
    Depositor
    Maarten Merkx
    Article
    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66
    Insert
    protein G - NanoLuc - NanoLuc
    Use
    Tags
    his-tag and strep-tag
    Expression
    Bacterial
    Mutation
    Ala 24 stop
    Promoter
    T7
    Available since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    169107-plasmid-map-sequence-id-334716

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  20. FB323

    Plasmid
    #203570
    Purpose
    Module for the expression of geneticin resistance and Nanoluciferase, and the expression of firefly luciferase under the Ppaf promoter
    Depositor
    Jose Marcos
    Article
    Gandia et al Microb Biotechnol. 2022 Feb;15(2):630-64
    Insert
    PgpdA:Nluc:Ttub::PtrpC:nptII:Ttub::Ppaf:Luc:Ttub
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    203570-plasmid-map-sequence-id-405312

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  24. pMSCV-IRES-GFP-Mouse-caspase-1/11a

    Plasmid
    #183391
    Purpose
    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.
    Insert
    CARD domain of mouse caspase-1 fused to catalytic domain of mouse caspase-11 (Casp1 Mouse, Synthetic)
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mutation
    Promoter
    MESV
    Available since
    July 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    183391-plasmid-map-sequence-id-361183

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  25. pMSCV-IRES-GFP-redesigned-human-Caspase-4_ANR261ENP_D315H_M318K_T352K

    Plasmid
    #183357
    Purpose
    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.
    Insert
    Caspase-4 with ICE activity (CASP4 Human)
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mutation
    ANR261ENP, D315H, M318K, T352K
    Promoter
    MESV
    Available since
    May 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    183357-plasmid-map-sequence-id-361152

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  26. pMSCV-IRES-GFP-Hybrid-caspase_canine-CARDs+mouse-Caspase-11-catalytic domain

    Plasmid
    #183396
    Purpose
    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.
    Insert
    CARD domains of canine caspase-1/4 fused to catalytic domain of mouse caspase-11
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mutation
    Promoter
    MESV
    Available since
    May 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    183396-plasmid-map-sequence-id-361120

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  27. pMSCV-IRES-GFP-Hybrid-caspase_canine-CARDs+human-Caspase-4-catalytic domain

    Plasmid
    #183395
    Purpose
    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.
    Insert
    CARD domains of canine caspase-1/4 fused to catalytic domain of human caspase-4
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mutation
    Promoter
    MESV
    Available since
    May 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    183395-plasmid-map-sequence-id-361114

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Showing: 801 - 850 of 14928 results