We narrowed to 1,640 results for: plasmid for e coli
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Plasmid#169566PurposeTier-1 vector encoding PhCMV-driven tetracycline dependent regulator protein TetR (PhCMV-TetR-pA).DepositorInsertPCMV-driven tetracycline repressor protein class B from transposon Tn10
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC-94N
Plasmid#53281PurposeContains crtI, crtY, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and produces beta-carotene in E. coli when complemented with a gene encoding geranylgeranyl diphosphate synthaseDepositorInsertcrtY, crtI, crtB
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-PHYTipi
Plasmid#53283PurposeContains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces phytoene in E. coliDepositorInsertcrtE, idi, crtY, crtI, crtB
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-85b
Plasmid#53282PurposeContains crtE, crtI, crtY genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and will produce beta-carotene in E. coli when complemented with a gene encoding phytoene synthaseDepositorInsertcrtE, crtY, crtI
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-GGPPipi
Plasmid#53280PurposeContains crtE and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces geranylgeranyl diphosphate in E. coliDepositorInsertcrtE, idi
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-ZETAipi
Plasmid#53284PurposeContains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and pds (crtP) gene of Synechococcus PCC7942, and thereby produces zeta-carotene in E. coliDepositorInsertpds
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterlacAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET11b-MIF
Plasmid#149446PurposeExpression of WT MIF in E. coli for protein productionDepositorInsertMIF (MIF Human)
UseTagsExpressionBacterialMutationWTPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTCF236 HLA-B*40:01
Plasmid#180463PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-B*40:01
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTCF7 HLA-B*07:02
Plasmid#180446PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-B*07:02
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJA48 HLA-B*57:01
Plasmid#180444PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-B*57:01
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTCF37 HLA-B*35:01
Plasmid#180450PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-B*35:01
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTCF10 HLA-B*08:01
Plasmid#180447PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-B*08:01
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pE-SUMO CRYAA
Plasmid#80756PurposeExpression of human alpha A-crystallin in E. coliDepositorInsertCRYAA (CRYAA Human)
UseTags6xHIS and Smt3 SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET11b-MIF Y99F
Plasmid#149439PurposeExpression of MIF Y99F in E. coli for protein productionDepositorInsertMIF (MIF Human)
UseTagsExpressionBacterialMutationY99FPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
rnALDH
Plasmid#67920PurposeExpresses aldehyde dehydrogenase from Rattus norvegicus, codon optimized for expression in E. coliDepositorInsertRattus norvegicus aldehyde dehydrogenase (Aldh3a1 Rat)
UseTags6x-HisExpressionBacterialMutationPromoterT7Available sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
ptrc99a-GPPS-CSstr-ispA*[JBEI-15060]
Plasmid#100962PurposeContains genes encoding GPP synthase, cineole synthase and IspA(S81F)DepositorInsertGPPS-CSstr-ispA*
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pE-SUMO CRYBA2
Plasmid#80746PurposeExpression of human beta A2-crystallin in E. coliDepositorInsertCRYBA2 (CRYBA2 Human)
UseTags6xHIS and Smt3 SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET11b-MIF Y99A
Plasmid#149440PurposeExpression of MIF Y99A in E. coli for protein productionDepositorInsertMIF (MIF Human)
UseTagsExpressionBacterialMutationY99APromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11b-MIF H62F
Plasmid#149445PurposeExpression of MIF H62F in E. coli for protein productionDepositorInsertMIF (MIF Human)
UseTagsExpressionBacterialMutationH62FPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11b-MIF H62A
Plasmid#149443PurposeExpression of MIF H62A in E. coli for protein productionDepositorInsertMIF (MIF Human)
UseTagsExpressionBacterialMutationH62APromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11b-MIF H62Y
Plasmid#149442PurposeExpression of MIF H62Y in E. coli for protein productionDepositorInsertMIF (MIF Human)
UseTagsExpressionBacterialMutationH62YPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11b-MIF Y99G
Plasmid#149441PurposeExpression of MIF Y99G in E. coli for protein productionDepositorInsertMIF (MIF Human)
UseTagsExpressionBacterialMutationY99GPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6411
Plasmid#47050PurposeBglBrick plasmid (=pBbB8k-P450) coding for a cytochrome P450 and its associated reductases to oxidize limonene to perillyl alcohol in E. coliDepositorInsertahpG-ahpH-ahpI coding for cytochrome P450 (CYP153A6) and its associated ferredoxin and ferredoxin reductase
UseSynthetic Biology; BglbrickTagsExpressionBacterialMutationPromoterPbadAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTrc-trGPPS(CO)-LS
Plasmid#50603PurposepTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthaseDepositorInsertsLimonene Synthase
ApR
LacIq
UseSynthetic BiologyTagsExpressionBacterialMutationtruncated codon optimized geranylpyrophosphate sy…PromoterTrcAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1 METTL3
Plasmid#200731PurposeBacterial expression of GST-Tagged codon optimized human METTL3DepositorInsertMETTL3 (METTL3 Human)
UseTagsGST from backbone plasmidExpressionBacterialMutationCodon optimized for expression in E. coliPromoterAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGG-TurboID
Plasmid#209391PurposeTransiently expressing TurboID in plantaDepositorInsert3XHA-TurboID
UseTagsExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-TurboID-GFP
Plasmid#209399PurposeTransiently expressing and visualizing the subcellular localization of TurboID in plantaDepositorInsert3XHA-TurboID
UseTagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Gm:Rm_AF
Plasmid#66061PurposeMoClo Device: FACS Standard Color Controls - High GFP (first unit) & RFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E:J23102:B:BCD2:C:E1010m:D:B0015:F]DepositorInsertFACS Controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Rm:Gm_AF
Plasmid#66062PurposeMoClo Device: FACS Standard Color Controls - High RFP (first unit) & GFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E:J23102:B:BCD2:C:E0040m:D:B0015:F]DepositorInsertFACS Controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-nsp7 (SARS-CoV-2)
Plasmid#169186PurposeTo express His-SUMO-nsp7 (SARS-CoV-2) in E. coliDepositorInsert14His-SUMO-nsp7 (ORF1ab SARS-CoV-2)
UseTags14His-SUMO (Ulp1-cleavable)ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a_LYN
Plasmid#224238PurposeExpression of human LYN Kinase in E. coliDepositorInsertlyn (LYN Human)
UseTags6xHis and 6xHis-TEVExpressionBacterialMutationPromoterT7 promotorAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSMH1741
Plasmid#160930PurposeExpression of soluble Sc octamer (H3)DepositorInsertsHHF1
HTA1
HTB1
HHT1
UseTags6His-TEV, N-6His-TEV, N-terminal 6His-TEV, and no…ExpressionMutationcodon-optimized for E. coli -- see provided seque…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSMH1742
Plasmid#160929PurposeExpression of soluble Sc octamer (Cse4)DepositorInsertsHHF1
HTA1
HTB1
Cse4
UseTags6His-TEV, N-terminal 6His-TEV, and noneExpressionMutationcodon-optimized for E. coli -- see provided seque…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
PAS_RvSAHS6
Plasmid#215647PurposeExpresses SUMO-tagged RvSAHS6 protein from tardigrade R. varieornatus in E. coliDepositorInsertSAHS6
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PAS_RvSAHS1
Plasmid#215645PurposeExpresses SUMO-tagged RvSAHS1 protein from tardigrade R. varieornatus in E. coliDepositorInsertSAHS1
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTCF176 HLA-A*02:01 (CD8 mut)
Plasmid#180459PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-A*02:01 (CD8 mut)
UseTagsBSP41ExpressionBacterialMutationD227K, T228A, replacement of transmembrane domain…PromoterAvailable sinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T_CIP2A_1-560
Plasmid#217921PurposeE. coli expression of GST-tagged CIP2A_1-560 proteinDepositorInsertCIP2A (CIP2A Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTCF79 H-2D(b) (CD8 mut)
Plasmid#180453PurposeE. coli expression of soluble MHC proteinDepositorInsertH-2D(b) (CD8 mut)
UseTagsBSP41ExpressionBacterialMutationD227K, replacement of transmembrane domain with e…PromoterAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pE-SUMO CRYBA3
Plasmid#80747PurposeExpression of human beta A3-crystallin in E. coliDepositorInsertCRYBA3 (CRYBA1 Human)
UseTags6xHIS and Smt3 SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pE-SUMO CRYGS
Plasmid#80753PurposeExpression of human gamma S-crystallin in E. coliDepositorInsertCRYGS (CRYGS Human)
UseTags6xHIS and Smt3 SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC6
Plasmid#49793PurposeE. coli cloning vectorDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoternoneAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pE-SUMO CRYBA1
Plasmid#80745PurposeExpression of human beta A1-crystallin in E. coliDepositorInsertCRYBA1 (CRYBA1 Human)
UseTags6xHIS and Smt3 SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a_HCK
Plasmid#224239PurposeExpression of human HCK Kinase in E. coliDepositorInserthck (HCK Human)
UseTags6xHis and 6xHis-TEVExpressionBacterialMutationPromoterT7 promotorAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
PAS_HeSAHS4
Plasmid#215648PurposeExpresses SUMO-tagged HeSAHS4 protein from tardigrade H. exemplaris in E. coliDepositorInsertSAHS4
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PAS_RvSAHS4
Plasmid#215646PurposeExpresses SUMO-tagged RvSAHS4 protein from tardigrade R. varieornatus in E. coliDepositorInsertSAHS4
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJA12 Mamu-A1*001:01
Plasmid#180442PurposeE. coli expression of soluble MHC proteinDepositorInsertMamu-A1*001:01
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJA13 Mamu-A1*002:01
Plasmid#180443PurposeE. coli expression of soluble MHC proteinDepositorInsertMamu-A1*002:01
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTCF122 Mamu-B*008:01
Plasmid#180455PurposeE. coli expression of soluble MHC proteinDepositorInsertMamu-B*008:01
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC-ZEAXipi
Plasmid#53287PurposeContains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coliDepositorInsertcrtE, idi, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-CANTHipi
Plasmid#53301PurposeContains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus pluvialis fused to a Trc promoter. Produces canthaxanthin in E. coli.DepositorInsertcrtO
UseLow copy number bacterial cloning vectorTags6HisExpressionMutationPromoterTrcAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only