We narrowed to 7,125 results for: RAP

1. pLenti-PGK-RAP2A-WT-puro

   Plasmid

   #156170

   Purpose

   Lentiviral vector for expression of RAP2A-WT, with puromycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   wild type form

   Promoter

   human PGK

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-CMV-TIRAP-cGASΔN-HA

   Plasmid

   #130921

   Purpose

   Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the TIRAP N terminus (aa1-85); Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   TIRAP-ΔNcGAS
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   cGAS N terminus (aa1-159) replaced with the N ter…

   Promoter

   CMV

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. hSRAP.TWIN1.a105-236.V105M

   Plasmid

   #49819

   Purpose

   expression vector for SRAP(105-236)V105M in pTWIN1 vector

   Depositor

   Thomas Cech

   Article

   McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

   Insert

   steroid receptor RNA activator protein aa 105-236 (SRA1 Human)
   

   Use

   Tags

   intein & chitin binding domain

   Expression

   Bacterial

   Mutation

   V105M codon-optimized for E. coli expression

   Promoter

   T7

   Available since

   Jan. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. hSRAP.TWIN1.a105-215.V105M

   Plasmid

   #49818

   Purpose

   expression vector for SRAP(105-215)V105M in pTWIN1 vector

   Depositor

   Thomas Cech

   Article

   McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

   Insert

   steroid receptor RNA activator protein aa 105-215 (SRA1 Human)
   

   Use

   Tags

   intein & chitin binding domain

   Expression

   Bacterial

   Mutation

   V105M codon-optimized for E. coli expression

   Promoter

   T7

   Available since

   Jan. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET His10-Draper INT

   Plasmid

   #126670

   Purpose

   Expression of His10-Draper intracellular domain for purification from E. coli

   Depositor

   Ron Vale

   Article

   Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

   Insert

   Draper cytoplasmic domain
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. Gene Trap - 24XMS2 Lentiviral Vector

   Plasmid

   #174198

   Purpose

   Lentiviral expression vector coding for Blasticidin, iRFP and 24X MS2 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.

   Depositor

   Daniel Larson

   Article

   Wan et al Cell. 2021 May 27;184(11):2878-2895.e20.

   Insert

   iRFP
   

   Use

   Lentiviral

   Tags

   24X MS2 hairpins, Blasticidin, and T2A

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUSE-Src-YF-UniRapR-mCerulean-myc

   Plasmid

   #45381

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

   Insert

   Src-YF-UniRapR-mCerulean-myc
   

   Use

   Tags

   Myc, Myr, and mCerulean

   Expression

   Mammalian

   Mutation

   changed mSrc Tyrosine 529 to Phenylalanine

   Promoter

   CMV

   Available since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST-CMV 3xFLAG-GABARAP-GFP

   Plasmid

   #123111

   Purpose

   Expresses 3xFLAG-GABARAP-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAP

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
   

   Use

   Tags

   3xFLAG and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-CMV 3xFLAG-GABARAPL1-GFP

   Plasmid

   #123112

   Purpose

   Expresses 3xFLAG-GABARAPL1-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL1

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
   

   Use

   Tags

   3xFLAG and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAVS1_EF1a-MitoTrap-mCherry-IRES-Puro

    Plasmid

    #197067

    Purpose

    Donor plasmid for human AAVS1 locus knock-in, constitutive expression of MitoTrap-mCherry

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    MitoTrap-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-Duet1_6xHis-TEV-GABARAP-Gly

    Plasmid

    #190930

    Purpose

    Plasmid for the expression and purification of GABARAP. Internal Reference: SMC894

    Depositor

    Sascha Martens

    Article

    Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

    Insert

    GABARAP (GABARAP Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Ends with Glycine 116

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2

    Plasmid

    #212112

    Purpose

    Expression of C-terminally tagged GABARAP(G116A) with mTagBFP2

    Depositor

    Dieter Willbold

    Article

    Üffing et al Autophagy Reports, 3(1). doi: 10.1080/27

    Insert

    GABARAP(G116A)-mTagBFP2 (GABARAP Human, Entacmaea quadricolor)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    G116A (lipidation deficiency) of GABARAP

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Gene Trap - 24XPP7 Lentiviral Vector

    Plasmid

    #174199

    Purpose

    Lentiviral expression vector coding for Blasticidin, iRFP and 24X PP7 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.

    Depositor

    Daniel Larson

    Article

    Wan et al Cell. 2021 May 27;184(11):2878-2895.e20.

    Insert

    iRFP
    

    Use

    Lentiviral; Vector

    Tags

    24X PP7 hairpins, Blasticidin, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLPC FLAG hRap1 delta BR delta CT

    Plasmid

    #13257

    Depositor

    Titia de Lange

    Article

    Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

    Insert

    Repressor Activator Protein 1 delta Br delta CT (TERF2IP Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mutation

    contains hRap1 aa2-40 + 96-270 +331-399 and the p…

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pDEST-CMV 3xFLAG-GABARAPL2-GFP

    Plasmid

    #123113

    Purpose

    Expresses 3xFLAG-GABARAPL2-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL2

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
    

    Use

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-PGK-RAP1A-E63-puro

    Plasmid

    #156167

    Purpose

    Lentiviral vector for expression of RAP1A-E63, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1A (RAP1A Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    changed Glutamine 63 to Glutamic acid

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-PGK-RAP1A-E63-neo

    Plasmid

    #156174

    Purpose

    Lentiviral vector for expression of RAP1A-E63, with neomycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1A (RAP1A Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    changed Glutamine 63 to Glutamic acid

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBABE myc-Raptor S722A/S792A

    Plasmid

    #18117

    Depositor

    Reuben Shaw

    Article

    Gwinn et al Mol Cell. 2008 Apr 25. 30(2):214-26.

    Insert

    Raptor (RPTOR Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    S722A, S792A

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pUSE-Src-KD-UniRapR-mCerulean-myc

    Plasmid

    #45382

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

    Insert

    Src-KD-UniRapR-mCerulean-myc
    

    Use

    Tags

    mCerulean and myc

    Expression

    Mammalian

    Mutation

    mSrc R388D (kinase dead), Y529F

    Promoter

    CMV

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-PGK-RAP2A-V12-neo

    Plasmid

    #156178

    Purpose

    Lentiviral vector for expression of RAP2A-V12, with neomycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Valine

    Promoter

    human PGK

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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