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pGS_N_Strep

Plasmid

#101651

Purpose

Expression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, KpnI linker, Twin-Strep tag

Use

Tags

Twin-Strep tag

Expression

Insect

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGS_C_Strep

Plasmid

#101652

Purpose

Expression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, MCS linker, Twin-Strep tag

Use

Tags

Twin-Strep tag

Expression

Insect

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pSG5-FLAG-CaMKKbeta N-terminus on CaMKKalpha

Plasmid

#33330

Depositor

Anthony Means

Article

Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

Insert

Calcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

CaMKKbeta N-terminus (1-164) was fused to body fo…

Promoter

SV40

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

Plasmid

#172982

Purpose

control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

control sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-eDHFR(69K6)-MCS

Plasmid

#172100

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

EGFP-eDHFR(69K6)-20aa

Use

Tags

Expression

Mammalian

Mutation

eDHFR(69K6): Hexalysine (K6) sequence is inserted…

Promoter

CMV

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#2

Plasmid

#172984

Purpose

CRISPRi for TAOK2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

sgTAOK2i (TAOK2 Human)

Use

CRISPR and Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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SPLICS NU-MT P2A

Plasmid

#210129

Purpose

Detect the Nucleus-Mitochondria contact

Depositor

Tito Calì

Article

Wu et al EMBO J. 2022 Dec 15;41(24):e112006. doi:

Insert

SPLICS NU-MT P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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Flag‐hTim1

Plasmid

#49207

Purpose

expresses Flag-tagged human Tim1

Depositor

Lawrence Kane

Article

de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

Insert

Tim1 (HAVCR1 Human)

Use

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

Tim1 lacks its own signal sequence

Promoter

EF1a

Available since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_nV5-AMOTL2

Plasmid

#172999

Purpose

constitutive expression of V5-tagged AMOTL2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

V5-AMOTL2 (AMOTL2 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_nV5-AMOT (p130)

Plasmid

#172997

Purpose

constitutive expression of V5-tagged AMOT (p130)

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

V5-AMOT (p130) (AMOT Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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p-spTorA-GFP-H6C

Plasmid

#168517

Purpose

GFP with the signal peptide of TorA (spTorA) in pBAD24. Includes a C-terminal HHHHHHC (6xHisC) extension.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

spTorA-GFP-H6C

Use

Tags

6xHisC tag and spTorA (signal peptide of E. coli …

Expression

Bacterial

Mutation

The mut3 GFP variant with 5 additional mutations …

Promoter

araBAD

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_nV5-AMOTL1

Plasmid

#172998

Purpose

constitutive expression of V5-tagged AMOTL1

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

V5-AMOTL1 (AMOTL1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR1a-tagRFP-KDEL

Plasmid

#114177

Purpose

Gateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

Depositor

Nicolas Demaurex

Article

Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

Insert

tagRFP-KDEL

Use

Gateway entry clone

Tags

KDEL retention signal and signal peptide

Expression

Mutation

Promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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GST-PS-Grb2 1-154 (XSB382)

Plasmid

#78510

Purpose

Construct for expressing Grb2 lacking the C-terminal SH3 domain in E. Coli

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

Grb2 1-154 (GRB2 Human)

Use

Tags

GST followed by a prescission protease claevage s…

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-MCS-eDHFR(69K6)-EGFP

Plasmid

#172101

Purpose

Mammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFP

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

22aa-eDHFR(69K6)-EGFP

Use

Tags

Expression

Mammalian

Mutation

eDHFR(69K6): Hexalysine (K6) sequence is inserted…

Promoter

CMV

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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Flag‐mTim3

Plasmid

#49208

Purpose

expresses Flag tagged mouse Tim3

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 (Havcr2 Mouse)

Use

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

no Tim3 signal sequence

Promoter

EF1a

Available since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pMSCV-SOCS6-WT

Plasmid

#74496

Purpose

Overexpression of SOCS6 in mammalian cells

Depositor

Lars Rönnstrand

Article

Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

Insert

SOCS6 (Socs6 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

unknown

Available since

May 2, 2016

Availability

Academic Institutions and Nonprofits only
pHR-LAT Y6F YYYY-LAT YYYY -mCitrine (XSB588)

Plasmid

#78516

Purpose

Lentiviral vector for expressing LAT-Y6F YYYY-LAT-YYYY-mCitrine (3x2 Grb2 binding sites)

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

LAT (LAT Human)

Use

Lentiviral

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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Flag‐mTim1 (Bl/6)

Plasmid

#49205

Purpose

expresses Flag tagged mouse Tim1

Depositor

Lawrence Kane

Article

de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

Insert

Tim1 (BL/6 allele) (Havcr1 Mouse)

Use

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

Tim1 lacks its own signal sequence

Promoter

EF1a

Available since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only
pc-FLAGCSK-SH3-SH2 (2 to 190 amino acids)

Plasmid

#74505

Purpose

Overexpression of CSK SH3-SH2 in mammalian cells

Depositor

Lars Rönnstrand

Article

Kazi et al Cell Signal. 2013 Sep;25(9):1852-60. doi

Insert

CSK (CSK Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

SH3-SH2 domains

Promoter

CMV

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only