We narrowed to 4,074 results for: p2a

1. pUFlip-floxed2A-mRFP-; HE1A:BFP -1

   Plasmid

   #180008

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-mRFP; HE1A: BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUFlip-floxed2A-mRFP-; gcry1:BFP -1

   Plasmid

   #173887

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-mRFP; gcry1: BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-ATF5-PROX1-HNF1A

   Plasmid

   #149725

   Purpose

   expressing three liver promoting transcription factors (ATF5, PROX1, HNF1A)

   Depositor

   Baisong Lu

   Article

   Lu et al Biomaterials. 2021 Feb;269:120668. doi:

   Insert

   ATF5-PROX1-HNF1A (ATF5 Human)
   

   Use

   Lentiviral

   Tags

   P2A, T2A

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-AsCas12a-Plus-mCherry

   Plasmid

   #191231

   Purpose

   To express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificity

   Depositor

   Zhili Rong

   Article

   Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

   Insert

   AsCas12a-Plus-P2A-mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   E174R, R951K, R955A

   Promoter

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9-NG-APOBEC-nCas9-Ung

   Plasmid

   #198539

   Purpose

   Expresses human codon-optimized SpCas9-NG-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NG-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NG-APOBEC-nCas9-Ung
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAY15 (OsTIR1(F74G) mAID-EGFP-NLS)

   Plasmid

   #140534

   Purpose

   OsTIR1(F74G)-P2A-mAID-EGFP-NLS TOL2

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   OsTIR1(F74G) mAID-EGFP-NLS TOL2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAY7 (OsTIR1 mAID-EGFP-NLS TOL2)

   Plasmid

   #140533

   Purpose

   OsTIR1(WT)-P2A-mAID-EGFP-NLS TOL2

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   mAID-EGFP-NLS TOL2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNTI194

   Plasmid

   #84266

   Purpose

   Expresses Avi-tagged RPL1 and humanized birA

   Depositor

   Nicholas Ingolia

   Article

   Ingolia et al Cell Rep. 2014 Sep 11;8(5):1365-79. doi:

   Inserts

   RPL1 (RPL10A (*see below), Human)
   humanized birA
   

   Use

   Tags

   Avi tag, Flag, Gly/Ser linker, and TEV site

   Expression

   Mammalian

   Mutation

   R33A, A34E (*see below)

   Promoter

   CMV and P2A peptide

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Polymerase expression - pCMV-T7-EcKlenow (LM2692)

   Plasmid

   #208963

   Purpose

   Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   EcKlenow-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-hA3A-eBE-Y130F

    Plasmid

    #113423

    Purpose

    Expresses hA3A-eBE-Y130F in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3A-eBE-Y130F (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    hAPOBEC3A_Y130F

    Promoter

    CMV

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. LentiCRISPRv2Cre

    Plasmid

    #82415

    Purpose

    Lentiviral vector expressing Cre recombinase alongside Cas9 and an sgRNA cloning site

    Depositor

    David Feldser

    Article

    Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

    Insert

    Cre Recombinase
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Mutated BsmBI cut site

    Promoter

    EFS (P2A)

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiDP1.0

    Plasmid

    #171094

    Purpose

    lentiviral construct (3rd generation) for the expression of cell cycle-independent OsTIR1 fused with mEmerald and miniAID-fused mCherry2

    Depositor

    Dario Palmieri

    Article

    Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

    Insert

    OsTIR1-mEmerald-P2A-miniAID-mCherry2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiDP1.3

    Plasmid

    #171096

    Purpose

    lentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Cdt1 (AKA ROLECCS G1) and miniAID-fused mCherry2

    Depositor

    Dario Palmieri

    Article

    Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

    Insert

    OsTIR1-mEmerald-Cdt1-P2A-miniAID-mCherry2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Emerald, cdt1, miniAID, mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiDP1.2

    Plasmid

    #171095

    Purpose

    lentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (AKA ROLECCS G2) and miniAID-fused mCherry2

    Depositor

    Dario Palmieri

    Article

    Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

    Insert

    OsTIR1-mEmerald-Geminin-P2A-miniAID-mCherry2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Emerald, Geminin, miniAID, mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSH231-EF1-BLST-dCas9-VPR

    Plasmid

    #115148

    Purpose

    Safe harbor site 231 knock-in vector with BlastR-dCas9-VPR expression cassette

    Depositor

    Michael Phelps, Raymond Monnat

    Article

    Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

    Insert

    BlastR-P2A-dCas9-VPR
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV5-hBMPR-1A CA

    Plasmid

    #49527

    Purpose

    expresses constitutively active human BMP receptor 1A

    Depositor

    Peter ten Dijke, Lee Niswander

    Article

    Varley et al Dev Biol. 1998 Apr 1;196(1):107-18.

    Insert

    BMPR-1A CA (BMPR1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    constitutively active mutation (Q233D); also P2A …

    Promoter

    CMV

    Available since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. PE-P3-rice

    Plasmid

    #185612

    Purpose

    For plant prime editing in rice plants

    Depositor

    Jinxiao Yang

    Article

    Xu et al Nat Plants. 2022 Jan;8(1):45-52. doi: 10

    Insert

    3XFLAG-4XNLS-M-MLV-nCas9(H840A)-4XNLS-P2A-Hyg
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ZmUbi

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. gRNA3_NIPBL_Cas9-hGem-for_N-terminal_tagging

    Plasmid

    #217662

    Purpose

    expresses Cas9-hGem and guideRNA for N terminal tagging of NIPBL

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    sgRNA3 for N terminal tagging of NIPBL (NIPBL Human)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSBbi-NLSmCherryNLS Clover-HDHB

    Plasmid

    #190223

    Purpose

    Fluorescent cell cycle reporter

    Depositor

    Laura Heiser

    Article

    Gross et al Nat Commun. 2023 Jun 10;14(1):3450. doi:

    Inserts

    Human DNA Helicase B (amino acids 994-1087) (HELB Human)
    NLS-mCherry-NLS-P2A-Puromycin
    

    Use

    Transposon

    Tags

    Clover fluorescent protein

    Expression

    Mammalian

    Mutation

    Amino Acids 994-1087

    Promoter

    EF1a and RPBSA

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. A2_uSFFV

    Plasmid

    #153415

    Purpose

    Expresses HLA-A*02:01 MHCI gene by the UCOE-SFFV promoter

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    A*02:01-P2A-human beta2 microglobulin (B2M Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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