We narrowed to 21,779 results for: his

1. C514-E11: attB5r-RU5u-His6-nostop-attB1r

   Plasmid

   #162953

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   His6 purification tag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. C417-E01: attB4-polyhedrinp>His6-nostop-attB1r

   Plasmid

   #162932

   Purpose

   Gateway attB4/attB1r entry clone for baculovirus expression of His6 tagged proteins using the polyhedrin promoter

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   His6 fusion with AcMNPV polyhedrin promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-Coh-ddFLN4-ELP(MV7E2)3-His-ybbR

   Plasmid

   #157672

   Purpose

   Expression of Coh (type III from Ruminococcus flavefaciens) with ddFLN4, ELP linker, His6x tag, and ybbR Tag.

   Depositor

   Michael Nash

   Article

   Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

   Insert

   Cohesin (R.f) with ddFLN4, ELP, Histag and ybbR tag
   

   Use

   Tags

   ddFLN4, ELP, Histag and ybbR tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a-ybbR-His-ELP(MV7E2)3-ddFLN4-SpyCatcher

   Plasmid

   #157674

   Purpose

   Expression of SpyCatcher with ddFLN4, ELP linker, His6x tag, and ybbR Tag.

   Depositor

   Michael Nash

   Article

   Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

   Insert

   SpyCatcher with ddFLN4, ELP, Histag and ybbR tag
   

   Use

   Tags

   ddFLN4, ELP, Histag and ybbR tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-ybbr-XMod-Doc (BMB-KO, S199AzF)-HIS

   Plasmid

   #153445

   Purpose

   E. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc.XDocB
   

   Use

   Tags

   6xHis and ybbr tag

   Expression

   Bacterial

   Mutation

   Serine 199 was mutated to amber codon (TAG). Argi…

   Promoter

   T7 promoter

   Available since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-ybbr-XMod-Doc (BMA-KO, S199AzF)-HIS

   Plasmid

   #153444

   Purpose

   E. coli expression (amber suppression) of Rc. XDocB binding mode A knock-out mutant with serine at position 199 replaced with amber codon.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc.XDocB
   

   Use

   Tags

   6xHis and ybbr tag

   Expression

   Bacterial

   Mutation

   Serine 199 was mutated to amber codon (TAG). Argi…

   Promoter

   T7 promoter

   Available since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GB1-His-TET1-878-911-L900A(pL91)

   Plasmid

   #124395

   Purpose

   E.Coli produced TET1-SID mutants (L900A) purified by FPLC nickel column

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   GB1-His-TET1-878-911-L900A (TET1 Human)
   

   Use

   Tags

   GB1-Histidine

   Expression

   Bacterial

   Mutation

   L900A

   Promoter

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GB1-His-TET1-878-911-T898E(pL91)

   Plasmid

   #124394

   Purpose

   E.Coli produced TET1-SID mutants (T898E) purified by FPLC nickel column

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   GB1-His-TET1-878-911-T898E (TET1 Human)
   

   Use

   Tags

   GB1-Histidine

   Expression

   Bacterial

   Mutation

   T898E

   Promoter

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GB1-His-TET1-878-911-T898A(pL91)

   Plasmid

   #124393

   Purpose

   E.Coli produced TET1-SID mutants (T898A) purified by FPLC nickel column

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   GB1-His-TET1-878-911-T898A (TET1 Human)
   

   Use

   Tags

   GB1-Histidine

   Expression

   Bacterial

   Mutation

   T898A

   Promoter

   Available since

   May 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GB1-His-TET1-878-911-L897A(pL91)

    Plasmid

    #124392

    Purpose

    E.Coli produced TET1-SID mutants (L897A) purified by FPLC nickel column

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    GB1-His-TET1-878-911-L897A (TET1 Human)
    

    Use

    Tags

    GB1-Histidine

    Expression

    Bacterial

    Mutation

    L897A

    Promoter

    Available since

    May 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GB1-His-TET1-878-911-I894A(pL91)

    Plasmid

    #124391

    Purpose

    E.Coli produced TET1-SID mutants (I894A) purified by FPLC nickel column

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    GB1-His-TET1-878-911-I894A (TET1 Human)
    

    Use

    Tags

    GB1-Histidine

    Expression

    Bacterial

    Mutation

    I894A

    Promoter

    Available since

    May 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-P90C

    Plasmid

    #114606

    Purpose

    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P90C cysteine mutations and a 42 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    dNt17 (deletion of first 17 amino acids), Q18C, P…

    Promoter

    Available since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-P70C

    Plasmid

    #114629

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 49 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, P70C; 49 polyQ repeat

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-P80C

    Plasmid

    #114602

    Purpose

    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P80C cysteine mutations and a 22 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    dNt17 (deletion of first 17 amino acids), Q18C, P…

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTWIN1-His6-Ssp-Httex1-23Q-A2C-P90C

    Plasmid

    #114615

    Purpose

    Bacterial expression of Htt fragment containing exon 1, with A2C & P90C cysteine mutations and a 23 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, P90C; 23 polyQ repeat

    Promoter

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-A60C

    Plasmid

    #114628

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 49 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, A60C; 49 polyQ repeat

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-A60C

    Plasmid

    #114601

    Purpose

    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & A60C cysteine mutations and a 22 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    dNt17 (deletion of first 17 amino acids), Q18C, A…

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-P70C

    Plasmid

    #114605

    Purpose

    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P70C cysteine mutations and a 42 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    dNt17 (deletion of first 17 amino acids), Q18C, P…

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTWIN1-His6-Ssp-Htt2-90(A2C)-23Q-P80C

    Plasmid

    #114614

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 23 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, P80C; 23 polyQ repeat

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTWIN1-His6-Ssp-Htt2-90(A2C)-43Q-P70C

    Plasmid

    #114624

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 43 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, P70C; 43 polyQ repeat

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-A60C

    Plasmid

    #114604

    Purpose

    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & A60C cysteine mutations and a 42 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    dNt17 (deletion of first 17 amino acids), Q18C, A…

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-P90C

    Plasmid

    #114603

    Purpose

    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P90C cysteine mutations and a 22 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    dNt17 (deletion of first 17 amino acids), Q18C, P…

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTWIN1-His6-Ssp-Httex1-43Q-A2C-P90C

    Plasmid

    #114625

    Purpose

    Bacterial expression of Htt fragment containing exon 1, with A2C & P90C cysteine mutations and a 43 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, P90C; 43 polyQ repeat

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-P90C

    Plasmid

    #114630

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 49 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, P90C; 49 polyQ repeat

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P90C

    Plasmid

    #114610

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 15 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, P90C; 15 polyQ repeat

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P70C

    Plasmid

    #114609

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 15 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, P70C; 15 polyQ repeat

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-P90C

    Plasmid

    #114620

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 37 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, P90C; 37 polyQ repeat

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-P80C

    Plasmid

    #114619

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P80C cysteine mutations and a 37 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, P80C; 37 polyQ repeat

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-A60C

    Plasmid

    #114618

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 37 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, A60C; 37 polyQ repeat

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-A60C

    Plasmid

    #114608

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 15 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, A60C; 15 polyQ repeat

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge