We narrowed to 39,938 results for: ANT

1. pLJM60-FLAG-SLC38A9.1 (delta110)

   Plasmid

   #71861

   Purpose

   lentiviral stable expression

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Jan 9;347(6218):188-94. do

   Insert

   SLC38A9.1 (SLC38A9 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon optimized, missing first 110 amino acids

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

2. Flag-Jun4A-Myc

   Plasmid

   #47444

   Purpose

   mammalian expression of mutant c-Jun (four N-terminal residues phosphorylated by JNK are mutated into Alanines)

   Depositor

   Axel Behrens

   Article

   Aguilera et al Nature. 2011 Jan 13;469(7329):231-5. doi

   Insert

   Jun 4A mutant (Jun Mouse)
   

   Tags

   Flag and myc

   Expression

   Mammalian

   Mutation

   four N-terminal residues phosphorylated by JNK, s…

   Promoter

   CMV

   Available Since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CHRM3-DuET

   Plasmid

   #213209

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CHRM3 (CHRM3 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CALCRL-DuET

   Plasmid

   #213194

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CALCRL (CALCRL Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ADORA1-DuET

   Plasmid

   #213173

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   ADORA1 (ADORA1 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. BDKBR1-DuET

   Plasmid

   #213189

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   BDKBR1 (BDKRB1 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HCTR2-DuET

   Plasmid

   #213311

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   HCTR2 (HCRTR2 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-ITR-EcYtR(NGS-6)-mCherry

   Plasmid

   #217363

   Purpose

   Expresses E. coli tyrosine tRNA variant "NGS-6" and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

   Insert

   E. coli tyrosine tRNA mutant, "NGS-6"
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Alpha MHC-DN.JNK2

   Plasmid

   #51931

   Purpose

   mammalian expression of dominant negative JNK2

   Depositor

   Jeffery Molkentin

   Article

   Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.

   Insert

   DN JNK2 (Mapk9 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   dominant negative: the dual phosphorylation moti…

   Promoter

   cardiac-specific α-myosin heavy chain 5.5 kb pro…

   Available Since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pAAV.hSynap-FLEX.SF-iGluSnFR.S72A

    Plasmid

    #106182

    Purpose

    Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.S72A
    

    Use

    AAV

    Mutation

    GltI: S72A

    Promoter

    hSynapsin

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HTR6-DuET

    Plasmid

    #213326

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    HTR6 (HTR6 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRetroX-TRE3G_FLAG-CRTC1/MAML2_PGK-GpNLuc

    Plasmid

    #154265

    Purpose

    Dox/Tet-inducible expression of the CRTC1-MAML2 fusion oncogene in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

    Insert

    FLAG-CRTC1/MAML2 fusion oncogene
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLJM60-FLAG-SLC38A9.1 (1-119aa)

    Plasmid

    #71862

    Purpose

    lentiviral stable expression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    SLC38A9.1 (SLC38A9 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized, first 119 amino acids

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3-CARMA2sh-HA

    Plasmid

    #97072

    Purpose

    Expresses CARMA2sh/CARD14 isoform 3 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CaMKIV-DCTK75E

    Plasmid

    #126423

    Purpose

    A kinase-dead mutant of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

    Depositor

    Douglas Black

    Article

    Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

    Insert

    calcium/calmodulin-dependent protein kinase IV kinase-dead mutant (CAMK4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed lysine 75 to glutamic acid

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Agarabi CHO Heavy Chain in pJ201

    Plasmid

    #111567

    Purpose

    Cloning vector containing CDS for the modified human heavy chain of the model chimeric mouse-human IgG1 expressed by the Agarabi CHO cell line

    Depositor

    Cyrus Agarabi

    Article

    Velugula-Yellela et al Biotechnol Prog. 2018 Jan;34(1):262-270.

    Insert

    Human heavy chain
    

    Use

    Synthetic Biology; Cloning vector

    Promoter

    None

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GPR141-DuET

    Plasmid

    #213263

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR141 (GPR141 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GPR20-DuET

    Plasmid

    #213275

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR20 (GPR20 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLPC-Progerin-CS

    Plasmid

    #69064

    Purpose

    encodes Progerin with a substitution of cysteine in CaaX-motif by a serine

    Depositor

    Gerardo Ferbeyre

    Article

    Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

    Insert

    Progerin (LMNA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Progerin with substitution of cysteine in CaaX-mo…

    Promoter

    CMV

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

20. GPR1-DuET

    Plasmid

    #213257

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR1 (GPR1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HTR2C-DuET

    Plasmid

    #213323

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    HTR2C (HTR2C Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. HCTR1-DuET

    Plasmid

    #213310

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    HCTR1 (HCRTR1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. HTR2A-DuET

    Plasmid

    #213321

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    HTR2A (HTR2A Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-CARMA2sh-FLAG

    Plasmid

    #97071

    Purpose

    Expresses CARMA2sh/CARD14 isoform 3 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV.CAG-FLEX.SF-iGluSnFR.A184V

    Plasmid

    #106187

    Purpose

    Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.A184V
    

    Use

    AAV

    Mutation

    GltI: A184V

    Promoter

    CAG

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV.GFAP.SF-iGluSnFR.A184V

    Plasmid

    #106193

    Purpose

    Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.A184V
    

    Use

    AAV

    Mutation

    GltI: A184V

    Promoter

    GFAP

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HTR4-DuET

    Plasmid

    #213324

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    HTR4 (HTR4 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mouse a5 full length

    Plasmid

    #217810

    Purpose

    Full length mouse integrin a5 expression

    Depositor

    Timothy Springer

    Article

    Yuxin et al bioRxiv 2024.01.26.577394

    Insert

    ITGA5 (Itga5 Mouse)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MCHR1-DuET

    Plasmid

    #213337

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MCHR1 (MCHR1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUPD2 sgRNAscaffold 2.1 scRNA (GB1437)

    Plasmid

    #160571

    Purpose

    Version of SgRNA scaffold with the sequence 2.1 of Ms2 aptamer in the 3' end.

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    sgRNAscaffold 2.1 scRNA
    

    Use

    CRISPR

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. HA-USPL1-C236A-pcDNA3.1

    Plasmid

    #85764

    Purpose

    mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236A

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, full length, catalytic inactive variant variant C236A (USPL1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    catalytic inactive mutant C236A

    Promoter

    CMV

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. HA-USPL1-C236S-pcDNA3.1

    Plasmid

    #85763

    Purpose

    mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236S

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, full length, catalytic inactive variant variant C236S (USPL1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    catalytic inactive mutant C236S

    Promoter

    CMV

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. mTFP1-Farnesyl-5

    Plasmid

    #55481

    Purpose

    Localization: Plasma Membrane, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    Farnesyl (HRAS Human)
    

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

34. DRD5-DuET

    Plasmid

    #213231

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    DRD5 (DRD5 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pPGK-T7/2-CD44v6-10 (-cyt)

    Plasmid

    #137821

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v6-10 (-cyt) (CD44 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region

    Promoter

    PGK

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. GAL3-DuET

    Plasmid

    #213249

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GAL3 (GALR3 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. GPR19-DuET

    Plasmid

    #213274

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR19 (GPR19 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLJM60-FLAG-SLC38A9.1 I68A

    Plasmid

    #71864

    Purpose

    lentiviral stable expression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    SLC38A9.1 (SLC38A9 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized, Isoleucine 68 mutated to Alanine

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

39. pAAV.GFAP.SF-iGluSnFR.S72A

    Plasmid

    #106194

    Purpose

    Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.S72A
    

    Use

    AAV

    Mutation

    GltI: S72A

    Promoter

    GFAP

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. CHRM5-DuET

    Plasmid

    #213211

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CHRM5 (CHRM5 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. CHRM4-DuET

    Plasmid

    #213210

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CHRM4 (CHRM4 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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42. pAAV-EF1a-CCre-MBP4-WPRE

    Plasmid

    #193919

    Purpose

    Expresses one of the components of Cre-DOR_N5C4 (RFP-dependent Cre)

    Depositor

    Tatsushi Onaka

    Article

    Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

    Insert

    CCre-MBP4
    

    Use

    AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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43. pAAV-EF1a-NCre-MBP5-WPRE

    Plasmid

    #193918

    Purpose

    Expresses one of the components of Cre-DOR_N5C4 (RFP-dependent Cre)

    Depositor

    Tatsushi Onaka

    Article

    Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

    Insert

    NCre-MBP5
    

    Use

    AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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44. pX131

    Plasmid

    #167149

    Purpose

    MoClo-compatible Level 0-SP promoterless vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    4'-phosphopantetheinyl transferase, npgA
    

    Use

    Synthetic Biology

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. pRK5-myc-Miro2 S430N

    Plasmid

    #47899

    Purpose

    Expresses myc tagged Miro2 S430N dominant negative mutant

    Depositor

    Pontus Aspenström

    Article

    Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

    Insert

    Miro2 S430N (RHOT2 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    S430N, dominant negative mutant

    Promoter

    CMV

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

46. pPGK-T7/2-CD44v9 (-cyt)

    Plasmid

    #137819

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v9 (-cyt) (CD44 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region

    Promoter

    PGK

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. MO91-ORAI1N223A-TEV-pHluorin (ORAI1-EC-pHluorin)

    Plasmid

    #174336

    Purpose

    The ORAI1N223A mutant containing-TEV-pHluorin-TEV sequence in the extracellular loop 2

    Depositor

    Sonal Srikanth

    Article

    Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

    Insert

    human Orai1 (ORAI1 Human)
    

    Use

    Retroviral

    Tags

    pHluorin inserted in the extracellular loop 2 of …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. pcDNA3-CARMA2cl-FLAG

    Plasmid

    #97077

    Purpose

    Expresses CARMA2cl/CARD14 isoform 2 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

    Insert

    CARD14 isoform 2 (CARD14 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. pX028

    Plasmid

    #167151

    Purpose

    MoClo-compatible Level 1 (position 2) vector encoding Neonothopanus nambi hispidin synthase nnHispS under control of 0.4 kb 35S promoter, for expression in plants

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    p35s-nnHispS-ocsT
    

    Expression

    Plant

    Promoter

    p35s

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. mTFP1-MAPTau-N-10

    Plasmid

    #55498

    Purpose

    Localization: Microtubules, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    MAPTau (MAPT Human)
    

    Tags

    mTFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only