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C-SWAT IAA17

Plasmid

#193319

Purpose

C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron). ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

IAA17

Tags

IAA17

Expression

Yeast

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT Compatible pAIDA2(6FLAG)

Plasmid

#193321

Purpose

C-SWAT compatible vector for C-terminal tagging with TAP-AID*-6FLAG. osTIR1 is carried downstream of the C-terminal tag.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

TAP-AID*-6FLAG

Tags

TAP-AID*-6FLAG

Expression

Yeast

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT AID*-6MYC osTIR1

Plasmid

#193320

Purpose

C-SWAT compatible vector for C-terminal tagging with AID*-6MYC for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

AID*

Tags

6MYC

Expression

Yeast

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT AID*-6FLAG osTIR1

Plasmid

#182515

Purpose

C-SWAT compatible vector for C-terminal tagging with AID*-6FLAG for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

AID*

Tags

6FLAG

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT IAA17-6FLAG

Plasmid

#182516

Purpose

C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

IAA17

Tags

6FLAG

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT IAA17-3HA

Plasmid

#182517

Purpose

C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin inducible degron) and 3HA. ARF16(PB1)-P2A-osTIR1 with SIR4 homology arms for genome integration.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

IAA17

Tags

3HA

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT IAA17-3MYC

Plasmid

#182518

Purpose

C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin inducible degron) and 3MYC. ARF16(PB1)-P2A-osTIR1 with SIR4 homology arms for genome integration.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

IAA17

Tags

3MYC

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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SIR4 gRNA HIS

Plasmid

#182520

Purpose

Expression vector for gRNA directed against SIR4 ORF.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

SIR4 gRNA (SIR4 Budding Yeast)

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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CAN1 gRNA HIS

Plasmid

#182521

Purpose

Expression vector for gRNA directed against CAN1 ORF.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

CAN1 gRNA (CAN1 Budding Yeast)

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT IAA17-6FLAG

Plasmid

#182522

Purpose

C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 with CAN1 homology arms for genome integration.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

IAA17

Tags

6FLAG

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_rSERT_X5C_S277C

Plasmid

#190177

Purpose

For mammalian cell expression of rat SERT and measurement of cytoplasmic pathway accessibility

Depositor

Gary Rudnick

Article

Zhang et al J Biol Chem. 2006 Nov 24;281(47):36213-2

Insert

rat serotonin transporter (Slc6a4 Rat)

Tags

FLAG, His6, and c-myc

Expression

Mammalian

Mutation

Cys15Ala, Cys21Ala, Cys109Ala, Ser277Cys, Cys357I…

Promoter

CMV

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-T11HA-hMOV10-WT

Plasmid

#178907

Purpose

expression of WT hMOV10

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

cDNA WT hMOV10 (MOV10 Human)

Tags

T11 beta strand of GFP-HA

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA6-mtIF3-Dendra2

Plasmid

#182371

Purpose

Dendra2-based translation reporter

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Insert

mtIF3 (Mtif3 Mouse)

Tags

Pal-Dendra2

Expression

Mammalian

Promoter

CMV promoter

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMCRW2

Plasmid

#127380

Purpose

pBC with 4 repeats of 80bp DNA template

Depositor

Richard C. Willson

Article

Chavan et al Analyst. 2020 Jul 21;145(14):4942-4949.

Insert

4 repeats of 80 bp DNA template sequence

Expression

Bacterial

Promoter

lac promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCL21-decoder

Plasmid

#159448

Purpose

Insert: Pact1-lexA-ER-B112-phosphodegron-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is degraded upon addition of alpha factor. Backbone: pRG201 (addGene). Marker: Met15.

Depositor

Joerg Stelling

Article

Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

Insert

Pact1-lexA-ER-B112-phosphodegron-tCyc1

Use

Synthetic Biology

Expression

Yeast

Promoter

act1

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCL38-decoder

Plasmid

#159452

Purpose

Insert: Pfus1tet-lexA-ER-B112-tCyc1.The transcription factor LexA-ER-B112 (see addgene FRP880) is inducible by alpha factor, and repressible by TetR. Backbone: pRG235 (addgene). Marker: Leu2

Depositor

Joerg Stelling

Article

Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

Insert

Pfus1tet-LexA-ER-B112-tCyc1

Use

Synthetic Biology

Expression

Yeast

Promoter

Pfus1tet

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCL12-decoder

Plasmid

#159447

Purpose

Insert: PlexAtet-citrine-tCyc1. The fluorescent protein Citrine is inducible by LexA-ER-B112 upon addition of estradiol, and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.

Depositor

Joerg Stelling

Article

Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

Insert

PlexAtet-Citrine-tCyc1

Use

Synthetic Biology

Expression

Yeast

Promoter

PlexAtet

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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NdeI – SacII – SpeI(NheI) - ANXA2 (29-339)

Plasmid

#136545

Purpose

Expresses ANXA2 chimeras in bacterial cells

Depositor

Laszlo Nyitray

Article

Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

Type

Empty backbone

Tags

ANXA2 (29-339) and His-tag

Expression

Bacterial

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pXF90

Plasmid

#139044

Purpose

MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

halotag::HDEL (includes STOP codon, PATC introns)

Expression

Worm

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pZF-BBB

Plasmid

#127470

Purpose

coding sequence for synthetic zinc finger ZF-BBB

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BBB synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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