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pZDonor-Nm-Cas9-gRNA-hU6

Plasmid

#61366

Purpose

empty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNA

Depositor

Charles Gersbach

Article

Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-c/o NL4-3 Env delta CT

Plasmid

#139285

Purpose

HIV-1 NL4-3 glycoprotein lacking cytoplasmic tail

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

codon optimized HIV-1 Env delta cytoplasmic tail

Expression

Mammalian

Mutation

deletion of cytoplasmic tail after residue 710

Promoter

CMV IE

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAV103

Plasmid

#63193

Purpose

pRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Worm and Yeast

Available Since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

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P[acman]-Alpha2

Plasmid

#165859

Purpose

Alpha2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pRetroSuper-shSrc

Plasmid

#26982

Depositor

Joan Massague

Article

Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

Insert

shRNA Src (SRC Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pMP71-mGFP-LLO-mPMEL

Plasmid

#174608

Purpose

expresses palmitoylated GFP with a C terminal fusion of the LLO190 epitope and the murine hPMEL epitope

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

membrane GFP fused to LLO and mouse PMEL antigen

Expression

Mammalian

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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LB701: pMVP/PB/mCherry-DEST

Plasmid

#121871

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ mCherry selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IY601: pMVP/Lenti/Blast-DEST

Plasmid

#121849

Purpose

pMVP destination vector, empty Lentivirus vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Lentiviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pILGFPE9

Plasmid

#83545

Purpose

Used to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF1 promoter-yEGFP

Expression

Yeast

Promoter

TEF1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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LC301: pMVP/PB/Zeo-DEST

Plasmid

#121877

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Zeo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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STAgR_tdTomato

Plasmid

#102993

Purpose

Can be used as backbone for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Type

Empty backbone

Use

CRISPR; Pcr template for stagr vectors

Promoter

U6

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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LNCX BMP-RII

Plasmid

#12641

Depositor

Rik Derynck

Article

Skillington et al J Cell Biol. 2002 Oct 14. 159(1):135-46.

Insert

BMP-RII (BMPR2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Oct. 17, 2006

Availability

Academic Institutions and Nonprofits only
pENTR4 TNRC6B

Plasmid

#20022

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Trinucleotide repeat containing 6B (TNRC6B Human)

Use

Entry vector

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-FlicR1

Plasmid

#74143

Purpose

Express FlicR1 preferentially in neurons

Depositor

Robert Campbell

Article

Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

Insert

FlicR1

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pVenus-N-SLC35B2

Plasmid

#155038

Purpose

Transient expression of SLC35B2-Venus

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 (SLC35B2 Human)

Tags

Venus

Expression

Mammalian

Mutation

See depositor comments below

Promoter

CMV

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pVk7-33G1-Flag

Plasmid

#58950

Purpose

backbone for mouse recombinant antibody with added C-terminal FLAG tag

Depositor

Gavin Wright

Article

Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

Type

Empty backbone

Tags

FLAG tag (DYKDDDDK)

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pSaCas9-sgRNA-Tetra-com vector

Plasmid

#131227

Purpose

For expressing gRNA, whose Tetraloop is replaced with a com aptamer

Depositor

Baisong Lu

Article

Lyu et al Nucleic Acids Res. 2019 Jul 12. pii: 553

Insert

vector for com modified sgRNA

Use

AAV

Expression

Mammalian

Available Since

Oct. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPRai

Plasmid

#189938

Purpose

Expresses CRISPRai cassette under EF1a promoter

Depositor

Yu-Chen Hu

Article

Truong et al Nucleic Acids Res. 2019 Jul 26;47(13):e7

Insert

CRISPRai cassette

Expression

Mammalian

Promoter

CMV enhancer-rat EF1 alpha

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pFA-DAB2

Plasmid

#162690

Purpose

Expresses DabAB2 inorganic carbon transport complex

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

DabAB2 inorganic carbon transport complex

Expression

Bacterial

Promoter

PLtet0-1 promoter

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHis2-Arf1 (17-181)-Q71L

Plasmid

#184808

Purpose

Bacterial expression of Arf1 (aa17-181, Q71L mutation)

Depositor

James Hurley

Article

Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10

Insert

Arf1 (ARF1 Human)

Tags

His6

Expression

Bacterial

Mutation

aa 17-181 only, Q71L

Promoter

T7

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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