We narrowed to 42,145 results for: Eras
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Plasmid#84598PurposeRenilla Luciferase reporter assay for BCL2 3'UTR with mutations on miR-200c binding siteDepositorInsertBCL2 3'UTR (UTS2R Human)
UseLuciferaseTagsRenilla LuciferaseMutationMutation on miR-200c binding site on BCL2 3'…PromoterCMVAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIneoRL-TOB1 3'UTR-mir200c-mut
Plasmid#84590PurposeRenilla Luciferase reporter assay for TOB1 3'UTR with mutations on miR-200c binding siteDepositorInsertTOB1 3'UTR (UTS2R Human)
UseLuciferaseTagsRenilla LuciferaseMutationMutation on miR-200c binding site on TOB1 3'…PromoterCMVAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIneoRL-RanBP93'UTR-mir200c-mut
Plasmid#84592PurposeRenilla Luciferase reporter assay for RANBP9 3'UTR with mutations on miR-200c binding siteDepositorInsertRANBP9 3'UTR (RANBP9 Human)
UseLuciferaseTagsRenilla LuciferaseMutationMutation on miR-200c binding site on RANBP9 3…PromoterCMVAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pzs1[GalS-L]
Plasmid#60743PurposeContains PIq driving expression of GalS-R, the Fucose inducible chimera with the wt Lac DBD.DepositorInsertGalS-L
UseSynthetic BiologyExpressionBacterialMutationLacI/GalR repressor chimera. LacI DBD with GalS L…Available SinceJuly 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZS1[RbsR-L]
Plasmid#60741PurposeContains PIq driving expression RbsR-L, the Ribose inducible chimera with the wt Lac DBD.DepositorInsertRbsR-L
UseSynthetic BiologyExpressionBacterialMutationLacI/GalR repressor chimera. LacI DBD with RbsR L…Available SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRevLCav2deltaC
Plasmid#60809Purposeto make truncation of yeast Rev3DepositorInsertREV3 (REV3 Budding Yeast)
ExpressionYeastMutationrev3ΔC encodes for Rev3 lacking the C-terminus (a…Available SinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRevLCav2FeS
Plasmid#60808Purposeto disrupt iron sulfur cluster in yeast Rev3DepositorInsertREV3 (REV3 Budding Yeast)
ExpressionYeastMutationcontains 647 C-terminal amino acids and 335 bp of…Available SinceMarch 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-RNMT 1-120
Plasmid#112706PurposeExpresses N-terminally FLAG-tagged human RNMT 1-120 in mammalian cellsDepositorInsertRNMT (RNMT Human)
TagsFLAGExpressionMammalianMutationdeleted amino acids 121-476PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-RNMT 121-476
Plasmid#112707PurposeExpresses N-terminally FLAG-tagged human RNMT 121-476 in mammalian cellsDepositorInsertRNMT (RNMT Human)
TagsFLAGExpressionMammalianMutationdeleted amino acids 2-120PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL_LRRK2:2141-2527
Plasmid#210899PurposeInsect expression of human LRRK2 fragment T2141 to E2527. N-terminal 6X His tag with TEV cleavage.DepositorInsertLRRK2:T2141-E2527 (LRRK2 Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationM2397T natural variant ( VAR_024965)PromoterPolyhedrinAvailable SinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBiT3.1-N_LRRK2:2142-2527
Plasmid#211112PurposeMammalian expression of human LRRK2 fragment R2142 to E2527. N-terminal HiBiT tag.DepositorInsertLRRK2:R2142-E2527 (LRRK2 Human)
TagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationM2397T natural variant (VAR_024965)PromoterCMVAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_LRRK2:2142-2527
Plasmid#211151PurposeMammalian expression of human LRRK2 fragment R2142 to E2527. C-terminal HiBiT tag.DepositorInsertLRRK2:R2142-E2527 (LRRK2 Human)
TagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationM2397T natural variant ( VAR_024965)PromoterCMVAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBD-BirA_LRRK2:2141-2527
Plasmid#210901PurposeInsect expression of human LRRK2 fragment T2141 to E2527. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertLRRK2:T2141-E2527 (LRRK2 Human)
TagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationM2397T natural variant ( VAR_024965)PromoterPolyhedrinAvailable SinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBacMam2-DiEx-LIC_LRRK2:2141-2527
Plasmid#210900PurposeInsect expression of human LRRK2 fragment T2141 to E2527. C-terminal 10X His and Flag tag with TEV cleavage site.DepositorInsertLRRK2:T2141-E2527 (LRRK2 Human)
TagsAENLYFQSHHHHHHHHHHDYKDDDDK and MExpressionInsectMutationM2397T natural variant ( VAR_024965)PromoterP10 and CMVAvailable SinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSP6-RNAP
Plasmid#48143PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylADepositorInsertrna polymerase SP6
UseSynthetic BiologyExpressionBacterialMutationV314A (numbering based on NCBI Sequence ID: NP_85…Available SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
IFN-Beta_pGL3
Plasmid#102597PurposeIFN-beta promoter driving luciferase. Use in IFN reporter assay.DepositorAvailable SinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL1521 (pSPIN, pSC101* backbone)
Plasmid#160729PurposeSingle-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.DepositorInsertVchCAST proteins, crRNA, and donor DNA
ExpressionBacterialPromoterJ23119Available SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTET2-lucCP-3MS2
Plasmid#198351PurposePart of Firefly reporter system, encodes Firefly luciferase with MS2 stem loops in the 3'UTRDepositorInsertFirefly Luciferase
UseLuciferaseExpressionMammalianPromoterpTET2Available SinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTET2-RlucCP-3MS2
Plasmid#198354PurposePart of Renilla reporter system, encodes Renilla luciferase with MS2 stem loops in the 3'UTRDepositorInsertRenilla Luciferase
UseLuciferaseExpressionMammalianPromoterpTET2Available SinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
CBE-T1.14
Plasmid#193277PurposeExpression of CBE-T1.14 base editor in mammalian cells, CMV promoterDepositorInsertCBE-T1.14
ExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable SinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only